水溶性丙磺舒
水溶性丙磺舒
引用和文献 (21)
Invitrogen™

水溶性丙磺舒

丙磺舒通常用于抑制细胞膜中的有机阴离子转运蛋白。这些转运蛋白可以将染料和指示剂排出细胞，在利用细胞内保留的染料或指示剂进行的检测中，这会导致加载不良或背景信号偏高。Di Virgilio 等人 (1990) 较早描述了丙磺舒在封闭细胞内染料外排方面的应用，其已与多种阴离子染料和偶联物配合使用。常用的游离酸形式的丙磺舒很难溶解，需要 1 M了解更多信息
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货号数量
P3640010 x 77 mg
货号 P36400
价格（CNY)
4,688.00
Each
添加至购物车
数量:
10 x 77 mg
价格（CNY)
4,688.00
Each
添加至购物车
丙磺舒通常用于抑制细胞膜中的有机阴离子转运蛋白。这些转运蛋白可以将染料和指示剂排出细胞，在利用细胞内保留的染料或指示剂进行的检测中，这会导致加载不良或背景信号偏高。Di Virgilio 等人 (1990) 较早描述了丙磺舒在封闭细胞内染料外排方面的应用，其已与多种阴离子染料和偶联物配合使用。常用的游离酸形式的丙磺舒很难溶解，需要 1 M NaOH 才能使其溶解。我们的水溶性丙磺舒 (P36400) 可快速在检测缓冲液中溶解，而且无需使用腐蚀性的 NaOH。水溶性丙磺舒包含在 Fluo-4 NW 钙含量检测试剂盒（F36205、F36206）中，也可单独提供。

了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›

仅供科研使用。不可用于诊断程序。
规格
数量10 x 77 mg
建议的储存条件室温保存
运输条件室温
物理形态实心
产品类型细胞标记试剂
Unit SizeEach

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (21)

引用和文献
Abstract
The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.
Authors:Geng Y, Hoke A, Delpire E,
Journal:J Biol Chem
PubMed ID:19307180
'NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information. In a recent study, it was shown that peripheral nerve injury results in increased NKCC1 activity, not due to an increase in cotransporter expression, but to increased phosphorylation of the cotransporter (Pieraut, S., ... More
A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.
Authors:Clementi EA, Marks LR, Duffey ME, Hakansson AP,
Journal:J Biol Chem
PubMed ID:22700972
'To cause colonization or infection, most bacteria grow in biofilms where differentiation and death of subpopulations is critical for optimal survival of the whole population. However, little is known about initiation of bacterial death under physiological conditions. Membrane depolarization has been suggested, but never shown to be involved, due to ... More
Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers.
Authors:Di Virgilio F, Steinberg TH, Silverstein SC
Journal:Cell Calcium
PubMed ID:2191781
Fura-2 is widely used to measure the concentration of cytosolic free calcium, but in many cells the dye does not remain localized within the cytoplasmic matrix. In these cells, Fura-2 is sequestered within intracellular organelles, secreted into the extracellular medium, or both. We have found that, in mouse peritoneal macrophages, ... More
Genetic divergence of rotavirus nonstructural protein 4 results in distinct serogroup-specific viroporin activity and intracellular punctate structure morphologies.
Authors:Hyser JM, Utama B, Crawford SE, Estes MK,
Journal:J Virol
PubMed ID:22357281
Nonstructural protein 4 (NSP4) viroporin activity is critical for the replication and assembly of serogroup A rotavirus (RVA); however, the dramatic primary sequence divergence of NSP4s across serogroups raises the possibility that viroporin activity is not a common feature among RVs. We tested for NSP4 viroporin activity from divergent strains, ... More
Cell-based potassium ion channel screening using the FluxOR assay.
Authors:Beacham DW, Blackmer T, O' Grady M, Hanson GT,
Journal:J Biomol Screen
PubMed ID:20208034
FluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel activity, the FluxOR Potassium Ion Channel Assay is based on the activation of a novel fluorescent dye. This indicator reports channel activity with a ... More
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