Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules.
Authors Rock K L; Gramm C; Rothstein L; Clark K; Stein R; Dick L; Hwang D; Goldberg A L;
JournalCell
PubMed ID8087844
'Reagents that inhibit the ubiquitin-proteasome proteolytic pathway in cells have not been available. Peptide aldehydes that inhibit major peptidase activities of the 20S and 26S proteasomes are shown to reduce the degradation of protein and ubiquitinated protein substrates by 26S particles. Unlike inhibitors of lysosomal proteolysis, these compounds inhibit the ... More
Characterization of an iron-responsive promoter in the protozoan pathogen Trichomonas vaginalis.
Authors Tsai Chu-Dang; Liu Hsing-Wei; Tai Jung-Hsiang;
JournalJ Biol Chem
PubMed ID11741916
Iron has been shown to regulate transcription in the protozoan pathogen Trichomonas vaginalis. In this study, a DNA transfection system was developed to monitor ap65-1 promoter activity in response to changing iron supply. In conjunction with electrophoretic mobility shift assay, iron-induced transcription of the ap65-1 gene was shown to be ... More
Expression of human fibroblast growth factor 2 mRNA is post-transcriptionally controlled by a unique destabilizing element present in the 3'-untranslated region between alternative polyadenylation sites.
Authors Touriol C; Morillon A; Gensac M C; Prats H; Prats A C;
JournalJ Biol Chem
PubMed ID10409702
Fibroblast growth factor 2 (FGF-2) belongs to a family of 18 genes coding for either mitogenic differentiating factors or oncogenic proteins, the expression of which must be tightly controlled. We looked for regulatory elements in the 5823-nucleotide-long 3'-untranslated region of the FGF-2 mRNA that contains eight potential alternative polyadenylation sites. ... More
Coactivator-vitamin D receptor interactions mediate inhibition of the atrial natriuretic peptide promoter.
Authors Chen S; Cui J; Nakamura K; Ribeiro R C; West B L; Gardner D G;
JournalJ Biol Chem
PubMed ID10809746
We have discovered a role for coactivators binding to the AF-2 surface of the vitamin D receptor (VDR) in its negative effects on gene transcription. We tested nine amino acid residues (Ser(235), Ile(242), Lys(246), Asp(253), Ile(260), Leu(263), Leu(417), Leu(419), and Glu(420)) in human VDR which, based on homology to the ... More