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Invitrogen
Phospho-ULK1 (Ser556) Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102856
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ULK1(Accession O75385), corresponding to amino acid residues around phosphorylated Ser556.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of ULK1 only when phosphorylated at S556.
Target Information
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG1; ATG 1; ATG1; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1
Gene Aliases: AU041434; KIAA0722; mKIAA0722; ULK1; Unc51.1
UniProt ID: (Mouse) O70405
Entrez Gene ID: (Rat) 360827, (Mouse) 22241
molecular_function
protein kinase activity
protein serine/threonine kinase activity
ATP binding
Rab GTPase binding
protein kinase binding
protein complex binding
nucleotide binding
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Hsp90 protein binding
non-receptor serine/threonine protein kinase
autophagosome assembly
Ras protein signal transduction
axonogenesis
protein localization
regulation of autophagy
positive regulation of autophagy
positive regulation of macroautophagy
cerebellar granule cell differentiation
radial glia guided migration of cerebellar granule cell
neuron projection regeneration
neuron projection development
receptor internalization
cellular response to nutrient levels
response to starvation
protein autophosphorylation
negative regulation of collateral sprouting
axon extension
regulation of neurotrophin TRK receptor signaling pathway
protein phosphorylation
autophagy
signal transduction
regulation of gene expression
regulation of cell death
phosphorylation
peptidyl-serine phosphorylation
mitophagy in response to mitochondrial depolarization
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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