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NDE1 Polyclonal Antibody
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FIGURE: 1 / 3
NDE1 Antibody (PA5-119857) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat cerebellum tissue using NDE1 Polyclonal Antibody (Product # PA5-119857). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the NDE1 antibody at a dilution of ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-119857
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human NDE1 aa 1-200.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Rat brain tissue lysates, rat cerebellum tissue, human liver tissue.
Subcellular Location: Cytoskeleton, centrosome, spindle, kinetochore, cleavage furrow.
Target Information
NDE1 is s a member of the nuclear distribution E (NudE) family of proteins. It is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. NDE1 protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations to the gene lead to Lissencephaly 4 (LIS4) and Microhydranencephaly (MHAC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coiled-coil protein; epididymis secretory sperm binding protein; LIS1-interacting protein NUDE1; LIS1-interacting protein NUDE1, rat homolog; nuclear distribution gene E homolog 1; nuclear distribution protein nudE homolog 1-like; nudE nuclear distribution E homolog 1; nudE nuclear distribution gene E homolog 1; rNudE; unnamed protein product
Gene Aliases: HOM-TES-87; LIS4; MHAC; NDE; NUDE; NUDE1
UniProt ID: (Human) Q9NXR1, (Rat) Q9ES39
Entrez Gene ID: (Human) 54820, (Rat) 83836
establishment of mitotic spindle orientation
neuron migration
microtubule nucleation
chromosome segregation
mitotic centrosome separation
neuroblast proliferation
cell migration
cerebral cortex development
forebrain development
microtubule organizing center organization
vesicle transport along microtubule
chromosome localization
centrosome duplication
centrosome localization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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