Invitrogen

NEK2 Polyclonal Antibody

Name: NEK2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-15337
Availability: InStock

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说明书

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实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

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NEK2 Antibody (PA5-15337) in WB

Western blot analysis of NEK2 in HeLa cell lysate. Samples were incubated with NEK2 polyclonal antibody (Product # PA5-15337).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NEK2 Antibody — NEK2 signaling is mediated through the regulation of EZH2 protein stability in GSCs. (A and B) Immunoblotting (IB) for IP using a NEK2 antibody (A) or an EZH2 antibody (B) in 528 glioma spheres. IgG served as a control. (C) qRT-PCR for NEK2 and EZH2 expression in 528 glioma spheres transduced with shNEK2 no. 1, shNEK2 no. 2, or shNT (n = 3). *P < 0.05 and P > 0.05 (NS), by 1-way ANOVA followed by Dunnett's post-hoc test. (D) Western blotting for NEK2, EZH2, p-EZH2 (Ser21), and H3K27me3 expression in 528 glioma spheres transduced with shNEK2 no. 1, shNEK2 no. 2, or shNT. (E) ICC for NEK2 and EZH2 in 528 glioma spheres transduced with shNEK2 no. 1, shNEK2 no. 2, or shNT. Scale bars: 5 µm. (F and G) Western blotting for EZH2 in CHX-treated 528 glioma spheres pretransduced with shNEK2 no. 2 or shNT (F). EZH2 had a shorter half-life in the presence of silenced NEK2 (G). n = 3. **P < 0.01, by 1-way ANOVA. (H) The absence of NEK2 increased the ubiquitination of EZH2 in 528 and 267 glioma spheres. (I) Western blotting for EZH2 expression in MG132-treated 528 glioma spheres pretransduced with shNEK2 no. 2 or shNT. (J) An in vitro clonogenicity assay indicated that NEK2 silencing decreased the clonogenicity of 267 glioma spheres, which could be partially rescued by EZH2 overexpression (n = 10). **P < 0.01 and ***P < 0.001, by ELDA. (K) Western blotting indicated that NEK2 silencing decreased EZH2 expression in 267 glioma spheres, which could be rescued by NEK2 WT but not K37R-mutant ov {KD}

Immunogen image

Immunogen Image NEK2 Polyclonal Antibody

产品信息

PA5-15337

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 350-382 amino acids from the central region of human NEK2

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

800 µg

纯化类型

Ammonium sulfate precipitation, Size-exclusion - Dialysis

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2150367

靶标信息

NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: HSPK 21; NEK 2; NEK 2A; Never in mitosis A-related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK 1; Serine/threonine-protein kinase Nek2

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基因别名: NEK2; NEK2A; NLK1

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Entrez Gene ID: (Human) 4751

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NEK2 Polyclonal Antibody

NEK2 Antibody (PA5-15337) in WB

产品信息

PA5-15337

免疫印迹 (WB)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

规格

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

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