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Invitrogen
ADAM17 Polyclonal Antibody
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FIGURE: 1 / 4
ADAM17 Antibody (PA5-19872) in ICC/IF
Immunocytochemistry of HeLa cells using ADAM17 Polyclonal Antibody (Product # PA5-19872) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-19872
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 17 amino acid peptide near the carboxy terminus of human TACE
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is Hela cell lysate.
A 17 amino acid peptide near the carboxy terminus of human TACE.
The PA5-19872 immunogen is located within the last 50 amino acids of TACE.
Predicted molecular ~ 93kD. In Western blot applications, this antibody has been observed to detect a band at: 93-125kD (Post-modification: 9 N-linked glycosylation).
Predicted species reactivity based on immunogen sequence: mouse (94.1%).
Target Information
ADAM17 (disintegrin and metalloproteinase domain-containing protein 17) cleaves the membrane bound precusor of TNF-alpha to its mature soluble form. ADAM 17 is responsbile for the proteolytical release of soluble JAM3 from endothelial cells surface and the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. It acts as an activator of the Notch pathway by mediating cleavage of Notch and generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Mutations in the gene can result in inflammatory skin and bowel disease, neonatal 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a disintegrin and metallopeptidase domain 17; a disintegrin and metalloprotease domain 17; a disintegrin and metalloproteinase; a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); ADAM; ADAM 17; ADAMs; CD156b; CD156b antigen; Disintegrin and metalloproteinase domain-containing protein 17; metalloendopeptidases; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme
Gene Aliases: Adam17; TACE
UniProt ID: (Rat) Q9Z1K9
Entrez Gene ID: (Rat) 57027
response to hypoxia
positive regulation of protein phosphorylation
germinal center formation
proteolysis
membrane protein ectodomain proteolysis
cell adhesion
epidermal growth factor receptor signaling pathway
Notch signaling pathway
Notch receptor processing
integrin-mediated signaling pathway
positive regulation of cell proliferation
positive regulation of T cell chemotaxis
B cell differentiation
positive regulation of cell growth
positive regulation of cell migration
positive regulation of transforming growth factor beta receptor signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
response to lipopolysaccharide
negative regulation of interleukin-8 production
positive regulation of chemokine production
regulation of mast cell apoptotic process
T cell differentiation in thymus
cell adhesion mediated by integrin
wound healing, spreading of epidermal cells
epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway
response to drug
negative regulation of apoptotic process
positive regulation of epidermal growth factor-activated receptor activity
spleen development
cell motility
PMA-inducible membrane protein ectodomain proteolysis
positive regulation of cellular component movement
response to high density lipoprotein particle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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