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Invitrogen
RAP1GAP Polyclonal Antibody
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RAP1GAP Antibody (PA5-87968) in WB
Western blot analysis of RAP1GAP in extracts of various cell lines. Samples were incubated with RAP1GAP Polyclonal antibody (Product # PA5-87968) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-87968
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 508-727 of human Rap1GAP (NP_0011391301)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.56 mg/mL
规格
156 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: AMGLSNKKPN TVSTSHSGSF APNNPDLAKA AGISLIVPGK SPTRKKSGPF GSRRSSAIGI ENIQEVQEKR ESPPAGQKTP DSGHVSQEPK SENSSTQSSP EMPTTKNRAE TAAQRAEALK DFSRSSSSAS SFASVVEETE GVDGEDTGLE SVSSSGTPHK RDSFIYSTWL EDSVSTTSGG SSPGPSRSPH PDAGKLGDPA CPEIKIQLEA SEQHMPQLGC; Positive Samples: U-87MG, 293T, SGC-7901, LO2; Cellular Location: Golgi apparatus membrane, Peripheral membrane protein
靶标信息
Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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