Separation of bioconjugated quantum dots using capillary electrophoresis.
AuthorsVicente G, Colón LA,
JournalAnal Chem
PubMed ID18278946
'Capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was used to separate different bioconjugated CdSe/ZnS quantum dots (QDs). The QD nanocrystals studied were conjugated to the biomolecules streptavidin, biotin, and immunoglobulin G. The bioconjugated QDs showed different electrophoretic mobilities, which appear to depend upon the biomolecule that is attached to ... More
Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay.
AuthorsHildebrandt N, Charbonnière LJ, Beck M, Ziessel RF, Löhmannsröben HG
JournalAngew Chem Int Ed Engl
PubMed ID16247812
Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy.
AuthorsCharbonnière LJ, Hildebrandt N, Ziessel RF, Löhmannsröben HG,
JournalJ Am Chem Soc
PubMed ID17002375
A time-resolved fluoro-immunoassay (TR-FIA) format is presented based on resonance energy transfer from visible emitting lanthanide complexes of europium and terbium, as energy donors, to semiconductor CdSe/ZnS core/shell nanocrystals (quantum dots, QD), as energy acceptors. The spatial proximity of the donor-acceptor pairs is obtained through the biological recognition process of ... More
Single particle quantum dot imaging achieves ultrasensitive detection capabilities for Western immunoblot analysis.
AuthorsScholl B, Liu HY, Long BR, McCarty OJ, O'Hare T, Druker BJ, Vu TQ,
JournalACS Nano
PubMed ID19453141
Substantially improved detection methods are needed to detect fractionated protein samples present at trace concentrations in complex, heterogeneous tissue and biofluid samples. Here we describe a modification of traditional Western immunoblotting using a technique to count quantum-dot-tagged proteins on optically transparent PVDF membranes. Counts of quantum-dot-tagged proteins on immunoblots achieved ... More