Authors:Keren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
Journal:Nat Cell Biol
PubMed ID:19767741
'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More
Circulating Bmp10 acts through endothelial Alk1 to mediate flow-dependent arterial quiescence.
Authors:Laux DW, Young S, Donovan JP, Mansfield CJ, Upton PD, Roman BL,
Journal:
PubMed ID:23863480
'Blood flow plays crucial roles in vascular development, remodeling and homeostasis, but the molecular pathways required for transducing flow signals are not well understood. In zebrafish embryos, arterial expression of activin receptor-like kinase 1 (alk1), which encodes a TGFß family type I receptor, is dependent on blood flow, and loss ... More
In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space.
Authors:Thorne RG, Nicholson C
Journal:Proc Natl Acad Sci U S A
PubMed ID:16567637
'Diffusion within the extracellular space (ECS) of the brain is necessary for chemical signaling and for neurons and glia to access nutrients and therapeutics; however, the width of the ECS in living tissue remains unknown. We used integrative optical imaging to show that dextrans and water-soluble quantum dots with Stokes-Einstein ... More
Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.
Authors:Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
Journal:Nano Lett
PubMed ID:17408303
Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The ... More
Intravital FLIM-FRET imaging reveals dasatinib-induced spatial control of src in pancreatic cancer.
Authors:Nobis M, McGhee EJ, Morton JP, Schwarz JP, Karim SA, Quinn J, Edward M, Campbell AD, McGarry LC, Evans TR, Brunton VG, Frame MC, Carragher NO, Wang Y, Sansom OJ, Timpson P, Anderson KI,
Journal:
PubMed ID:23749641
Cancer invasion and metastasis occur in a complex three-dimensional (3D) environment, with reciprocal feedback from the surrounding host tissue and vasculature-governing behavior. In this study, we used a novel intravital method that revealed spatiotemporal regulation of Src activity in response to the anti-invasive Src inhibitor dasatinib. A fluorescence lifetime imaging ... More