AuthorsKeren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
JournalNat Cell Biol
PubMed ID19767741
'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More
Circulating Bmp10 acts through endothelial Alk1 to mediate flow-dependent arterial quiescence.
AuthorsLaux DW, Young S, Donovan JP, Mansfield CJ, Upton PD, Roman BL,
Journal
PubMed ID23863480
'Blood flow plays crucial roles in vascular development, remodeling and homeostasis, but the molecular pathways required for transducing flow signals are not well understood. In zebrafish embryos, arterial expression of activin receptor-like kinase 1 (alk1), which encodes a TGFß family type I receptor, is dependent on blood flow, and loss ... More
In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space.
AuthorsThorne RG, Nicholson C
JournalProc Natl Acad Sci U S A
PubMed ID16567637
'Diffusion within the extracellular space (ECS) of the brain is necessary for chemical signaling and for neurons and glia to access nutrients and therapeutics; however, the width of the ECS in living tissue remains unknown. We used integrative optical imaging to show that dextrans and water-soluble quantum dots with Stokes-Einstein ... More
Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.
AuthorsRyman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
JournalNano Lett
PubMed ID17408303
Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The ... More
Intravital FLIM-FRET imaging reveals dasatinib-induced spatial control of src in pancreatic cancer.
AuthorsNobis M, McGhee EJ, Morton JP, Schwarz JP, Karim SA, Quinn J, Edward M, Campbell AD, McGarry LC, Evans TR, Brunton VG, Frame MC, Carragher NO, Wang Y, Sansom OJ, Timpson P, Anderson KI,
Journal
PubMed ID23749641
Cancer invasion and metastasis occur in a complex three-dimensional (3D) environment, with reciprocal feedback from the surrounding host tissue and vasculature-governing behavior. In this study, we used a novel intravital method that revealed spatiotemporal regulation of Src activity in response to the anti-invasive Src inhibitor dasatinib. A fluorescence lifetime imaging ... More
Dynamics and mechanisms of quantum dot nanoparticle cellular uptake.
AuthorsXiao Y, Forry SP, Gao X, Holbrook RD, Telford WG, Tona A,
JournalJ Nanobiotechnology
PubMed ID20550705
ABSTRACT: BACKGROUND: The rapid growth of the nanotechnology industry and the wide application of various nanomaterials have raised concerns over their impact on the environment and human health. Yet little is known about the mechanism of cellular uptake and cytotoxicity of nanoparticles. An array of nanomaterials has recently been introduced ... More
Mechanisms of quantum dot nanoparticle cellular uptake.
AuthorsZhang LW, Monteiro-Riviere NA,
JournalToxicol Sci
PubMed ID19414515
Due to the superior photoemission and photostability characteristics, quantum dots (QD) are novel tools in biological and medical applications. However, the toxicity and mechanism of QD uptake are poorly understood. QD nanoparticles with an emission wavelength of 655 nm are ellipsoid in shape and consist of a cadmium/selenide core with ... More
Peptide-MHC potency governs dynamic interactions between T cells and dendritic cells in lymph nodes.
AuthorsSkokos D, Shakhar G, Varma R, Waite JC, Cameron TO, Lindquist RL, Schwickert T, Nussenzweig MC, Dustin ML
JournalNat Immunol
PubMed ID17632517
T cells survey antigen-presenting dendritic cells (DCs) by migrating through DC networks, arresting and maintaining contact with DCs for several hours after encountering high-potency complexes of peptide and major histocompatibility complex (pMHC), leading to T cell activation. The effects of low-potency pMHC complexes on T cells in vivo, however, are ... More
Surface coatings determine cytotoxicity and irritation potential of quantum dot nanoparticles in epidermal keratinocytes.
AuthorsRyman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
JournalJ Invest Dermatol
PubMed ID16902417
Quantum dot (QD) nanoparticles have potential applications in nanomedicine as drug delivery vectors and diagnostic agents, but the skin toxicity and irritation potential of QDs are unknown. Human epidermal keratinocytes (HEKs) were used to assess if QDs with different surface coatings would cause differential effects on HEK cytotoxicity, proinflammatory cytokine ... More
Penetration of intact skin by quantum dots with diverse physicochemical properties.
AuthorsRyman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
JournalToxicol Sci
PubMed ID16443688
Skin is the largest organ of the body and is a potential route of exposure to engineered nanomaterials, but the permeability of the skin to these nanomaterials is unknown. We selected commercially available quantum dots (QD) of two core/shell sizes and shapes and three different surface coatings to determine if ... More
Single-cell transcriptomics reveals involution mimicry during the specification of the basal breast cancer subtype.
Authors
JournalCell Rep
PubMed ID33852842
Enhancement of cancer invasion and growth via the C5a-C5a receptor system: Implications for cancer promotion by autoimmune diseases and association with cervical cancer invasion.
'Autoimmune diseases are caused by immune complex-induced activation of the complement system and subsequent inflammation. Recent studies have revealed an association between autoimmune diseases and worse survival in patients with cancer; however, the underlying mechanism is still unknown. The C5a-C5a receptor (C5aR) system has been shown to enhance cancer activity ... More
External light activates hair follicle stem cells through eyes via an ipRGC-SCN-sympathetic neural pathway.
AuthorsFan SM, Chang YT, Chen CL, Wang WH, Pan MK, Chen WP, Huang WY, Xu Z, Huang HE, Chen T, Plikus MV, Chen SK, Lin SJ
JournalProc Natl Acad Sci U S A
PubMed ID29959210
'Changes in external light patterns can alter cell activities in peripheral tissues through slow entrainment of the central clock in suprachiasmatic nucleus (SCN). It remains unclear whether cells in otherwise photo-insensitive tissues can achieve rapid responses to changes in external light. Here we show that light stimulation of animals'' eyes ... More
Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-Transmitted Yersinia pestis.
AuthorsShannon JG, Hinnebusch BJ
JournalMethods Mol Biol
PubMed ID31177431
'The technique known as intravital microscopy (IVM), when used in conjunction with transgenic mice expressing fluorescent proteins in various cell populations, is a powerful tool with the potential to provide new insights into host-pathogen interactions in infectious disease pathogenesis in vivo. Yersinia pestis, the causative agent of plague, is typically ... More
Effector CD4
AuthorsWesthorpe CLV, Norman MU, Hall P, Snelgrove SL, Finsterbusch M, Li A, Lo C, Tan ZH, Li S, Nilsson SK, Kitching AR, Hickey MJ
JournalNat Commun
PubMed ID29467472
'Although effector CD4'
Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury.
AuthorsRusso MV, Latour LL, McGavern DB,
JournalNat Immunol
PubMed ID29662169
'Mild traumatic brain injury (mTBI) can cause meningeal vascular injury and cell death that spreads into the brain parenchyma and triggers local inflammation and recruitment of peripheral immune cells. The factors that dictate meningeal recovery after mTBI are unknown at present. Here we demonstrated that most patients who had experienced ... More
Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia.
Authors
JournalCancer Cell
PubMed ID28870739
α-Toxin Induces Platelet Aggregation and Liver Injury during Staphylococcus aureus Sepsis.
Authors
JournalCell Host Microbe
PubMed ID30033122
Minimally invasive molecular delivery into the brain using optical modulation of vascular permeability.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID21576460
Lymphangiogenic therapy prevents cardiac dysfunction by ameliorating inflammation and hypertension.
Authors
JournalElife
PubMed ID33200983
Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish.
Authors
JournalNat Commun
PubMed ID26790525
A protocol for characterizing the impact of collateral flow after distal middle cerebral artery occlusion.
Authors
JournalTransl Stroke Res
PubMed ID21593993
Plasma cell dynamics in the bone marrow niche.
Authors
JournalCell Rep
PubMed ID33567286
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.
Authors
JournalNat Protoc
PubMed ID31996843
Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
AuthorsShin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND
JournalDev Cell
PubMed ID31564611
The lymphatic system comprises blind-ended tubes that collect interstitial fluid and return it to the circulatory system. In mammals, unidirectional lymphatic flow is driven by muscle contraction working in conjunction with valves. Accordingly, defective lymphatic valve morphogenesis results in backflow leading to edema. In fish species, studies dating to the ... More
A network of trans-cortical capillaries as mainstay for blood circulation in long bones.
AuthorsGrüneboom A, Hawwari I, Weidner D, Culemann S, Müller S, Henneberg S, Brenzel A, Merz S, Bornemann L, Zec K, Wuelling M, Kling L, Hasenberg M, Voortmann S, Lang S, Baum W, Ohs A, Kraff O, Quick HH, Jäger M, Landgraeber S, Dudda M, Danuser R, Stein JV, Rohde M, Gelse K, Garbe AI, Adamczyk A, Westendorf AM, Hoffmann D, Christiansen S, Engel DR, Vortkamp A, Krönke G, Herrmann M, Kamradt T, Schett G, Hasenberg A, Gunzer M
JournalNat Metab
PubMed ID31620676
Closed circulatory systems (CCS) underlie the function of vertebrate organs, but in long bones their structure is unclear, although they constitute the exit route for bone marrow (BM) leukocytes. To understand neutrophil emigration from BM, we studied the vascular system of murine long bones. Here we show that hundreds of ... More
Docking protein-1 promotes inflammatory macrophage signaling in gastric cancer.
AuthorsLi T, Li B, Sara A, Ay C, Leung WY, Zhang Y, Dong Y, Liang Q, Zhang X, Weidner P, Gutting T, Behrens HM, Röcken C, Sung JJ, Ebert MP, Yu J, Burgermeister E
JournalOncoimmunology
PubMed ID31646096
Docking protein-1 (DOK1) is a tumor suppressor frequently lost in malignant cells, however, it retains the ability to control activities of immune receptors in adjacent stroma cells of the tumor microenvironment. We therefore hypothesized that addressing DOK1 may be useful for cancer immunotherapy. ... More
In Vivo Generation of Gut-Homing Regulatory T Cells for the Suppression of Colitis.
AuthorsXu Y, Cheng Y, Baylink DJ, Wasnik S, Goel G, Huang M, Cao H, Qin X, Lau KW, Chan C, Koch A, Pham LH, Zhang J, Li CH, Wang X, Berumen EC, Smith J, Tang X
JournalJ Immunol
PubMed ID31053627
Current therapies for gut inflammation have not reached the desired specificity and are attended by unintended immune suppression. This study aimed to provide evidence for supporting a hypothesis that direct in vivo augmentation of the induction of gut-homing regulatory T (Treg) cells is a strategy of expected specificity for the ... More
Distinct Spatio-Temporal Dynamics of Tumor-Associated Neutrophils in Small Tumor Lesions.
AuthorsSody S, Uddin M, Grüneboom A, Görgens A, Giebel B, Gunzer M, Brandau S
JournalFront Immunol
PubMed ID31293583
Across a majority of cancer types tumor-associated neutrophils (TAN) are linked with poor prognosis. However, the underlying mechanisms, especially the intratumoral behavior of TAN, are largely unknown. Using intravital multiphoton imaging on a mouse model with neutrophil-specific fluorescence, we measured the migration of TAN in distinct compartments of solid tumor ... More
A surgical orthotopic approach for studying the invasive progression of human bladder cancer.
AuthorsLorenzatti Hiles G, Cates AL, El-Sawy L, Day KC, Broses LJ, Han AL, Briggs HL, Emamdjomeh A, Chou A, Abel EV, Liebert M, Palmbos PL, Udager AM, Keller ET, Day ML
JournalNat Protoc
PubMed ID30683938
The invasion of bladder cancer into the sub-urothelial muscle and vasculature are key determinants leading to lethal metastatic progression. However, the molecular basis is poorly understood, partly because of the lack of uncomplicated and reliable models that recapitulate the biology of locally invasive disease. We developed a surgical grafting technique, ... More