Authors:Ballou B, Lagerholm BC, Ernst LA, Bruchez MP, Waggoner AS
Journal:Bioconjug Chem
PubMed ID:14733586
'Quantum dots having four different surface coatings were tested for use in in vivo imaging. Localization was successfully monitored by fluorescence imaging of living animals, by necropsy, by frozen tissue sections for optical microscopy, and by electron microscopy, on scales ranging from centimeters to nanometers, using only quantum dots for ... More
In vivo imaging of quantum dots.
Authors:Texier I, Josser V,
Journal:Methods Mol Biol
PubMed ID:19488714
'Noninvasive whole-body near-infrared fluorescence imaging is now acknowledged as a powerful method for the molecular mapping of biological events in live small animals such as mouse models. With outstanding optical properties such as high fluorescence quantum yields and low photobleaching rates, quantum dots (QDs) are labels of choice in the ... More
Imaging takes a quantum leap.
Authors:Lidke DS, Arndt-Jovin DJ
Journal:Physiology (Bethesda)
PubMed ID:15546848
Semiconducting nanocrystals, or quantum dots (QDs), have emerged as a new tool in physiological imaging, combining high brilliance, photostability, broad excitation but very narrow emission spectra, and surface chemistry compatible with biomolecular conjugation. In this review, we demonstrate the power of QDs in diverse applications, including long-term in vivo fluorescence ... More
In vivo excitation of nanoparticles using luminescent bacteria.
Authors:Dragavon J, Blazquez S, Rekiki A, Samson C, Theodorou I, Rogers KL, Tournebize R, Shorte SL,
Journal:Proc Natl Acad Sci U S A
PubMed ID:22615349
The lux operon derived from Photorhabdus luminescens incorporated into bacterial genomes, elicits the production of biological chemiluminescence typically centered on 490 nm. The light-producing bacteria are widely used for in vivo bioluminescence imaging. However, in living samples, a common difficulty is the presence of blue-green absorbers such as hemoglobin. Here we ... More
Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization.
Authors:Park C, Hwang IY, Sinha RK, Kamenyeva O, Davis MD, Kehrl JH,
Journal:Blood
PubMed ID:22039261
B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited ... More