AuthorsBallou B, Lagerholm BC, Ernst LA, Bruchez MP, Waggoner AS
JournalBioconjug Chem
PubMed ID14733586
'Quantum dots having four different surface coatings were tested for use in in vivo imaging. Localization was successfully monitored by fluorescence imaging of living animals, by necropsy, by frozen tissue sections for optical microscopy, and by electron microscopy, on scales ranging from centimeters to nanometers, using only quantum dots for ... More
In vivo imaging of quantum dots.
AuthorsTexier I, Josser V,
JournalMethods Mol Biol
PubMed ID19488714
'Noninvasive whole-body near-infrared fluorescence imaging is now acknowledged as a powerful method for the molecular mapping of biological events in live small animals such as mouse models. With outstanding optical properties such as high fluorescence quantum yields and low photobleaching rates, quantum dots (QDs) are labels of choice in the ... More
Imaging takes a quantum leap.
AuthorsLidke DS, Arndt-Jovin DJ
JournalPhysiology (Bethesda)
PubMed ID15546848
Semiconducting nanocrystals, or quantum dots (QDs), have emerged as a new tool in physiological imaging, combining high brilliance, photostability, broad excitation but very narrow emission spectra, and surface chemistry compatible with biomolecular conjugation. In this review, we demonstrate the power of QDs in diverse applications, including long-term in vivo fluorescence ... More
In vivo excitation of nanoparticles using luminescent bacteria.
AuthorsDragavon J, Blazquez S, Rekiki A, Samson C, Theodorou I, Rogers KL, Tournebize R, Shorte SL,
JournalProc Natl Acad Sci U S A
PubMed ID22615349
The lux operon derived from Photorhabdus luminescens incorporated into bacterial genomes, elicits the production of biological chemiluminescence typically centered on 490 nm. The light-producing bacteria are widely used for in vivo bioluminescence imaging. However, in living samples, a common difficulty is the presence of blue-green absorbers such as hemoglobin. Here we ... More
Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization.
AuthorsPark C, Hwang IY, Sinha RK, Kamenyeva O, Davis MD, Kehrl JH,
JournalBlood
PubMed ID22039261
B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited ... More
Persistent tissue kinetics and redistribution of nanoparticles, quantum dot 705, in mice: ICP-MS quantitative assessment.
AuthorsYang RS, Chang LW, Wu JP, Tsai MH, Wang HJ, Kuo YC, Yeh TK, Yang CS, Lin P
JournalEnviron Health Perspect
PubMed ID17805425
BACKGROUND: Quantum dots (QDs) are autofluorescent semiconductor nanocrystals that can be used for in vivo biomedical imaging. However, we know little about their in vivo disposition and health consequences. OBJECTIVES: We assessed the tissue disposition and pharmacokinetics of QD705 in mice. METHODS: We determined quantitatively the blood and tissue kinetics ... More
Distinct origins and molecular mechanisms contribute to lymphatic formation during cardiac growth and regeneration.
Authors
JournalElife
PubMed ID31702554
Live Imaging of Intracranial Lymphatics in the Zebrafish.
Authors
JournalCirc Res
PubMed ID33135960
Imaging blood vessels and lymphatic vessels in the zebrafish.
AuthorsJung HM, Isogai S, Kamei M, Castranova D, Gore AV, Weinstein BM
JournalMethods Cell Biol
PubMed ID27263409
'Blood vessels supply tissues and organs with oxygen, nutrients, cellular, and humoral factors, while lymphatic vessels regulate tissue fluid homeostasis, immune trafficking, and dietary fat absorption. Understanding the mechanisms of vascular morphogenesis has become a subject of intense clinical interest because of the close association of both types of vessels ... More
Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice.
Authors
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID23220372
Whole-body, real-time preclinical imaging of quantum dot fluorescence with time-gated detection.
Authors
JournalJ Biomed Opt
PubMed ID20059235
Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk.
Authors
JournalDevelopment
PubMed ID33547133
Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination.
Authors
JournalNat Commun
PubMed ID34911937
Plasma cell dynamics in the bone marrow niche.
Authors
JournalCell Rep
PubMed ID33567286
A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level.
AuthorsErapaneedi R, Belousov VV, Schäfers M, Kiefer F
JournalEMBO J
PubMed ID26598532
Hypoxia is an intensively investigated condition with profound effects on cell metabolism, migration, and angiogenesis during development and disease. Physiologically, hypoxia is linked to tissue homeostasis and maintenance of pluripotency. Hypoxia also contributes to pathologies including cardiovascular diseases and cancer. Despite its importance, microscopic visualization of hypoxia is largely restricted ... More
Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
AuthorsShin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND
JournalDev Cell
PubMed ID31564611
The lymphatic system comprises blind-ended tubes that collect interstitial fluid and return it to the circulatory system. In mammals, unidirectional lymphatic flow is driven by muscle contraction working in conjunction with valves. Accordingly, defective lymphatic valve morphogenesis results in backflow leading to edema. In fish species, studies dating to the ... More
Late developing cardiac lymphatic vasculature supports adult zebrafish heart function and regeneration.
AuthorsHarrison MR, Feng X, Mo G, Aguayo A, Villafuerte J, Yoshida T, Pearson CA, Schulte-Merker S, Lien CL
JournalElife
PubMed ID31702553
The cardiac lymphatic vascular system and its potentially critical functions in heart patients have been largely underappreciated, in part due to a lack of experimentally accessible systems. We here demonstrate that cardiac lymphatic vessels develop in young adult zebrafish, using coronary arteries to guide their expansion down the ventricle. Mechanistically, ... More