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引用和文献 (6)
Invitrogen™
Quant-iT™ 蛋白检测试剂盒
The Quant-iT Protein Assay Kit makes protein quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and了解更多信息
| 货号 | 数量 |
|---|---|
| Q33210 | 1 Kit |
货号 Q33210
价格（CNY)
2,346.00
Each
数量:
1 Kit
价格（CNY)
2,346.00
Each
The Quant-iT Protein Assay Kit makes protein quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent, load it into the wells of a microplate, add 1-20 μL of sample, mix, then measure the fluorescence. The assay is highly selective for protein and exhibits very little protein-to-protein variation. The assay is performed at room temperature, and the signal is stable for 3 hours. Common contaminants, such as salts, solvents, or DNA—but not detergents—are well tolerated in the assay. Quant-iT DNA Assay Kits (Q33120, Q33130) and a Quant-iT RNA Assay Kit (Q33140) are also available.
仅供科研使用。不可用于诊断程序。
规格
检测荧光蛋白检测
适用于（设备）微孔板读数仪
基因 ID (Entrez)3919448
套装内容浓缩的检测试剂、稀释缓冲液以及预稀释的 BSA 标准品
产品线Quant-iT
产品类型蛋白定量检测
数量1 Kit
样品体积1 至 20 uL
运输条件室温
储存要求在冷藏冰箱中储存（2° 至 8°C）。避光。
检测方法荧光
Unit SizeEach
内容与储存
避光储存在冷藏冰箱 (2–8°C) 中。
常见问题解答 (FAQ)
为什么我用Qubit Assay检测时会得到负的荧光值？
我有一个Quant-iT DNA试剂盒，而想在Qubit荧光计上使用。可以吗？
这些Quant-iT试剂盒的适用pH范围是多少？
我试图对一些带有荧光基团标记的DNA进行定量。这可行吗？
DNA长度对dsDNA检测结果有影响吗？
引用和文献 (6)
引用和文献
Abstract
Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain.
Journal:J Biol Chem
PubMed ID:17491020
Cigarette smoke is a potent inhibitor of pulmonary T cell responses, resulting in decreased immune surveillance and an increased incidence of respiratory tract infections. The alpha,beta-unsaturated aldehydes in cigarette smoke (acrolein and crotonaldehyde) inhibited production of interleukin-2 (IL-2), IL-10, granulocyte-macrophage colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha by human T
Functional selection of phagocytosis-promoting genes: cell sorting-based selection.
Journal:J Biomol Screen
PubMed ID:20660795
Phagocytosis is a critical host defense mechanism that clears invading pathogens, apoptotic cells, and cell debris; it is an essential process for normal development, tissue remodeling, immune response, and inflammation. Here, a functional selection strategy was used to isolate novel phagocytosis-promoting genes. After the retroviral transfer of mouse brain cDNA
Quantitation of protein.
Journal:Methods Enzymol
PubMed ID:19892168
The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research and development labs for applications ranging from enzymatic studies to providing data for biopharmaceutical lot release. Spectrophotometric protein quantitation assays are methods that use UV and visible spectroscopy to rapidly determine the concentration of
A comparison of protein quantitation assays for biopharmaceutical applications.
Journal:Mol Biotechnol
PubMed ID:17914170
Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representing various sizes of protein and
Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution.
Journal:Anal Chem
PubMed ID:19947594
Cystic fibrosis transmembrane conductance regulator (CFTR) functions as an ion channel in the apical plasma membrane of epithelial cells. Mutations in the gene coding for CFTR cause cystic fibrosis (CF). A major cellular dysfunction is insufficient apical plasma membrane expression of the protein. Its correction is important for developing new