Qubit™ Flex Fluorometers and Starter Kits
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Qubit™ Flex Fluorometers and Starter Kits
Invitrogen™

Qubit™ Flex Fluorometers and Starter Kits

Experience accurate, specific, and sensitive quantification of DNA, RNA, microRNA, protein, and endotoxin with the Qubit Flex Fluorometer. Measure up to eight samples simultaneously using Qubit assay kits for reproducible results in seconds.
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Catalog NumberTypeWarranty
Q33327FluorometerStandard Warranty
Q45894Quantitation Starter KitStandard Warranty
Q45893NGS Starter KitStandard Warranty
Catalog number Q33327
Price (CNY)
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Type:
Fluorometer
Warranty:
Standard Warranty

The Qubit Flex Fluorometer is designed for highly accurate and sensitive quantification of DNA, RNA, microRNA, protein, and endotoxin. This benchtop fluorometer offers the flexibility to directly measure the fluorescence of up to eight samples simultaneously, which reduces assay variability. Paired with Qubit reagents, the Qubit Flex Fluorometer is part of an optimized workflow generating highly accurate and reproducible results. The fluorometer has a large screen and intuitive user interface, making it easy to select and run your desired assay with results displayed in just a few seconds.

Features of the Qubit Flex Fluorometer include:

  • Improved throughput—measure multiple samples and quantify DNA, RNA, protein, and endotoxin up to 50% faster than single-sample readers
  • Flexibility—measure up to eight samples simultaneously using 1–20 μL of sample
  • Complete optimized system—works seamlessly with Qubit reagents to generate reliable, sensitive, and specific results
  • On-board calculators—quickly generates Qubit working solution preparation instructions and determines the molarity of your samples
  • Easy to use—a large 8-inch, state-of-the-art color touchscreen and intuitive user interface results in easy workflow navigation
  • Data export flexibility—data is transferred easily as a CSV or PDF using the included Wi-Fi dongle, a USB drive, or connection to your network drive
  • Several languages available—choices include English, French, Spanish, Italian, German, simplified Chinese, and Japanese
  • Verification test—a pre-loaded program is included to verify performance of the instrument
  • Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument

Higher throughput, less variability than single sample readers

The Qubit Flex Fluorometer has the flexibility to measure multiple DNA, RNA, protein, and endotoxin samples up to 50% faster than single-sample readers. In addition to saving time, the ability to measure eight samples simultaneously reduces variability, resulting in highly reproducible data. The fluorometer includes reagent, range, and molarity calculators, further reducing the time to results. The reagent and range calculators help to determine which reagent and what amount of sample to use to effectively quantify the DNA, RNA, or protein. The molarity calculator allows you to calculate the molarity of your samples based on nucleic acid length and the measured concentration.

Enables more accurate results than UV absorbance at low concentrations

The Qubit Flex Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, and excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit Flex Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the target molecule (DNA, RNA, protein, or endotoxin) in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Comprehensive warranty options

The Qubit Flex Fluorometer comes with a standard 1-year warranty that covers repair costs including shipping, parts, and labor. Service plans can be bundled with new instrument purchases to provide a 3-year warranty. These Qubit Flex Fluorometer packages are offered with two different types of bundled warranties:

  • Rapid Exchange (REX) warranty (3-year)
    • Replacement shipped out in ~1 business day for labs with critical timelines
    • Available with the Qubit Flex Fluorometer (Cat. No. A44984), Qubit Flex NGS Starter Kit (Cat. No. A45924), Qubit Flex Quantification Starter Kit (Cat. No. A45925), Qubit Flex Endotoxin Starter Kit (Cat. No. Q32894REXPKG) and Qubit Flex Fluorometer with Security Software (Cat. No. Q45895REXPKG)
  • AB Repair Center (ABRC) warranty (3-year)
    • Instrument repaired and returned in ~3 weeks, ideal for regulated labs required to maintain asset tagging consistency
    • Available with the Qubit Flex Fluorometer (Cat. No. A44985), Qubit Flex NGS Starter Kit (Cat. No. A45926), Qubit Flex Quantification Starter Kit (Cat. No. A45927), Qubit Flex Endotoxin Starter Kit (Cat. No. Q32894PKG) and Qubit Flex Fluorometer with Security Software (Cat. No. Q45895PKG)

21 CFR Part 11 Software Module

The Qubit Flex Security Software supports compliance with 21 CFR Part 11 FDA guidelines for security, auditing, and electronic (SAE) signatures using the SAE administration console. The 21 CFR Part 11 software can be purchased separately (Cat. No. Q31994).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)DNA Quantification, RNA Quantification, MicroRNA Quantification, Protein Quantification
IncludesQubit Flex Fluorometer with Standard Warranty, Qubit Flex Power Cord, Wi-Fi Dongle
ModelQubit Flex Fluorometer
Quantity1 fluorometer
Sample Volume1 to 20 μL
Shipping ConditionRoom Temperature
WarrantyStandard Warranty
Excitation Wavelength460 to 480 nm (Blue LED), 620 to 640 nm (Red LED)
Product LineQubit
TypeFluorometer
Unit SizeEach
Contents & Storage
Qubit Flex Fluorometers include:
•Qubit Flex Fluorometer (Cat. No. Q33327)
•WiFi Dongle (Cat. No. A26774)
•Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument

Qubit Flex Quantitation Starter Kits include:
•Qubit Flex Fluorometer (Cat. No. Q33327)
•Qubit Flex Assay Tube Strips, 125 strips (Cat. No. Q33252)
•Qubit 1X dsDNA HS Assay Kit, 100 assays (Cat. No. Q33230)
•Qubit 1X dsDNA HS Assay Kit, 100 assays (Cat. No. Q33265)
•Qubit RNA HS Assay Kit, 100 assays (Cat. No. Q32852)
•Qubit RNA BR Assay Kit, 100 assays (Cat. No. Q10210)
•Qubit Protein Assay Kit, 100 assays (Cat. No. Q33211)
•Qubit Flex System Verification Assay Kit (Cat. No. Q33254)
•Qubit Flex Reservoirs, 100 reservoirs (Cat. No. Q33253)
•WiFi Dongle (Cat. No. A26774)
•Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument

Qubit Flex NGS (Next-Generation Sequencing) Starter Kit includes:
•Qubit Flex Fluorometer (Cat. No. Q33327)
•Qubit Flex Assay Tubes Strips, 125 strips (Cat. No. Q33252)
•Qubit 1X dsDNA HS Assay Kit, 500 assays (Cat. No. Q33231)
•Qubit Flex Assay Reservoirs, 100 reservoirs (Cat. No. Q33253)
•WiFi Dongle (Cat. No. A26774)
•Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument

Frequently asked questions (FAQs)

Can the Qubit 1X dsDNA, broad range (BR) Assay Kit (Cat. No. Q33265) be used on the Qubit 3 Fluorometer?

No, this assay is only compatible with Qubit 4 Fluorometer (Cat. No. Q33226) and Qubit Flex Fluorometer (Cat. No. Q33327).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

When setting up standard measurements using the Qubit Flex Fluorometer, should I add the standard+reagent mix to all 8 tubes or just one?

You have the flexibility to run less than 8 standards on the Qubit Flex Fluorometer. However, you can only place samples into the wells that have been calibrated. For instance, if you run standards in 6 wells, you can only run samples in those 6 wells (or fewer).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

My Qubit Flex Fluorometer (Cat. No. Q33327) cannot detect the WiFi dongle. How can I resolve this?

This issue can be a result of the REV C Wifi dongle being incompatible with firmware versions before 1.6.0. Please check that you are on firmware version 1.6.0 or later.

If the issue persists, please contact Technical Support at techsupport@thermofisher.com.

Can I use the Qubit Endotoxin Detection Assay Kit on the Qubit 4 Fluorometer?

No, the Qubit Endotoxin Detection Assay Kit is designed for use with the Qubit Flex Fluorometer and is not currently for use on the Qubit 4 Fluorometer.

To use the Qubit Endotoxin Detection Assay Kit on the Qubit Flex Fluorometer, do I need to perform a software update ?

Yes, the endotoxin detection assay program will be available on Qubit Flex Software version 1.8.0 or later. The latest software is available under the Resources section of www.thermofisher.com/qubit