Qubit™ Flex Fluorometer with Standard Warranty - FAQs

View additional product information for Qubit™ Flex Fluorometers and Starter Kits - FAQs (A44984, A44985, Q33327, A45924, A45925, A45926, A45927, Q45893, Q45894)

12 product FAQs found

Can the Qubit 1X dsDNA, broad range (BR) Assay Kit (Cat. No. Q33265) be used on the Qubit 3 Fluorometer?

No, this assay is only compatible with Qubit 4 Fluorometer (Cat. No. Q33226) and Qubit Flex Fluorometer (Cat. No. Q33327).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

When setting up standard measurements using the Qubit Flex Fluorometer, should I add the standard+reagent mix to all 8 tubes or just one?

You have the flexibility to run less than 8 standards on the Qubit Flex Fluorometer. However, you can only place samples into the wells that have been calibrated. For instance, if you run standards in 6 wells, you can only run samples in those 6 wells (or fewer).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

My Qubit Flex Fluorometer (Cat. No. Q33327) cannot detect the WiFi dongle. How can I resolve this?

This issue can be a result of the REV C Wifi dongle being incompatible with firmware versions before 1.6.0. Please check that you are on firmware version 1.6.0 or later.

If the issue persists, please contact Technical Support at techsupport@thermofisher.com.

Can I use the Qubit Endotoxin Detection Assay Kit on the Qubit 4 Fluorometer?

No, the Qubit Endotoxin Detection Assay Kit is designed for use with the Qubit Flex Fluorometer and is not currently for use on the Qubit 4 Fluorometer.

To use the Qubit Endotoxin Detection Assay Kit on the Qubit Flex Fluorometer, do I need to perform a software update ?

Yes, the endotoxin detection assay program will be available on Qubit Flex Software version 1.8.0 or later. The latest software is available under the Resources section of www.thermofisher.com/qubit

Is it possible to design a custom assay on a Qubit Flex Fluorometer (Cat. No. Q33327)?

The Qubit Flex Fluorometer does not have the option for custom assays.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center

Can the Qubit Flex Assay Tube Strips (Cat. No. Q33252) be cut to run 1 sample or does the full tube strip need to be placed in the Qubit Flex Fluorometer (Cat. No. Q33327)?

Qubit Flex Assay Tube Strips (Cat. No. Q33252) can be cut to run 1 sample with the Qubit Flex Fluorometer (Cat. No. Q33327).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Is the Qubit Flex Fluorometer (Cat. No. Q33327) compatible with custom assays?

No, the Qubit Flex Fluorometer does not have custom assay capability.

The settings options on my Qubit instrument are greyed out and I am being prompted to sign in. How can I resolve this?

Our Qubit 4 and Qubit Flex instruments will now require users to sign in to access certain menu options (i.e., instrument settings, software update, and system verification). This is necessary to adhere to cybersecurity legal standards that were put in place in 2020. Only when signed in, are all functions of the device are accessible.

Instructions for login:
- Turn on the device
- From the home screen, touch the person icon on the top left corner
- Touch the "Get Started" button
- Touch the "Create Profile" button
- Enter information for username and pin
- Log in by touching the person icon and entering the user info in the sign in fields

Which is the best DNA or RNA quantification instrument?

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

When using the Qubit Flex Fluorometer, do I have to use all 8 samples/wells?

No, the Qubit Flex Fluorometer allows you to select which wells will be used for the experiment. The standards and sample will only be read in the selected wells. If the option to select wells is not available on your Qubit Flex, please update to the current firmware version (download firmware from Technical Resources for Qubit Fluorometers). This feature was introduced in version 1.5.0.