ViewRNA™ ISH Cell Assay Kit
ViewRNA™ ISH Cell Assay Kit
Invitrogen™

ViewRNA™ ISH Cell Assay Kit

ViewRNA™ ISH Cell Assay 是一种直接的荧光 RNA 原位杂交方法,可使用荧光显微镜或高内涵成像仪以单拷贝灵敏度和单细胞分辨率同时检测 1 至 4了解更多信息
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货号数量
QVC00011 kit
货号 QVC0001
价格(CNY)
21,293.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
21,293.00
Each
添加至购物车
ViewRNA™ ISH Cell Assay 是一种直接的荧光 RNA 原位杂交方法,可使用荧光显微镜或高内涵成像仪以单拷贝灵敏度和单细胞分辨率同时检测 1 至 4 个 RNA 靶标(mRNA 或非编码 RNA)。与通常受高背景和低灵敏度限制的传统 FISH 技术不同,该检测使用专有化学技术用于靶向特异性探针集(RNA FISH 探针),并使用分支 DNA 信号扩增 (bDNA) 来检测特异性信号。荧光 RNA 原位杂交检测分为四个主要步骤:样品制备、靶标杂交、信号扩增以及检测。该 ViewRNA ISH 分析试剂盒至多可检测 3 个 RNA ,但可以与 ViewRNA™ 740 Module 结合使用,以添加第 4 个探针。

必需的附加组件:
10 X PBS (货号 QVC0508)
ViewRNA™ - Detergent Solution QC(货号 QVC0509)
ViewRNA™ - Wash Buffer Set(货号 QG0507)
ViewRNA™ ISH Cell Accessory Kit(货号 QVC0700)旨在提供试剂盒中未提供的许多必需组分,以进行测定。
ViewRNA™ Probe Sets 不包含在检测中,而是用于 ViewRNA ISH Cell Assay。请访问我们的网站以查看 6,500 多种合成探针集 (Probe Sets) 的完整列表。根据要求,新的探针集 (Probe Sets) 可在不到两周的时间内设计和合成,而不需要附加成本。

添加第 4 个 RNA 探针:
ViewRNA™ ISH Cell 740 Module(货号 QVC0200),旨在与 ViewRNA™ ISH Cell Assay 一同使用,并允许分析 740 通道中的其他 RNA 靶标 (AlexaFluor 750)。请参见包装插页以获取试剂盒中所提供材料的完整列表。

已报告的应用
显微镜检查

仅供科研使用。不可用于诊断程序。
规格
数量1 kit
类型ISH 细胞检测试剂盒
Unit SizeEach
内容与储存
对于足以进行 1-plex 到 3-plex(AlexaFluor 488、546 和 647)24-孔板样式(24 份样品)测定的成分,使用盖玻片盖于载玻片上可以进行 96 次测定

常见问题解答 (FAQ)

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The ViewRNA ISH Cell Assay Kit user manual lists an accessory kit, Cat. No. QVC0700 which has been discontinued. Do you offer or suggest alternatives for the items in the accessory kit that have also been discontinued?

Please contact our Technical Support team at cellanalysis.support@thermofisher.com for the list of suggested alternatives.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.