Raphe serotonin neuron-specific oxytocin receptor knockout reduces aggression without affecting anxiety-like behavior in male mice only.
AuthorsPagani JH, Williams Avram SK, Cui Z, Song J, Mezey É, Senerth JM, Baumann MH, Young WS
Journal
PubMed ID25677455
'Serotonin and oxytocin influence aggressive and anxiety-like behaviors, though it is unclear how the two may interact. That the oxytocin receptor is expressed in the serotonergic raphe nuclei suggests a mechanism by which the two neurotransmitters may cooperatively influence behavior. We hypothesized that oxytocin acts on raphe neurons to influence ... More
Frequent proviral integration of the human betaretrovirus in biliary epithelium of patients with autoimmune and idiopathic liver disease.
AuthorsWang W, Indik S, Wasilenko ST, Faschinger A, Carpenter EJ, Tian Z, Zhang Y, Wong GK, Mason AL
Journal
PubMed ID25521721
'A human betaretrovirus (HBRV) has been linked with primary biliary cirrhosis (PBC) following the detection of viral particles in biliary epithelium by electron microscopy and cloning of the betaretrovirus genome from biliary epithelium and peri-hepatic lymph nodes. Evidence for viral infection was found in the majority of PBC patients'' peri-hepatic ... More
High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry.
AuthorsPorichis F, Hart MG, Griesbeck M, Everett HL, Hassan M, Baxter AE, Lindqvist M, Miller SM, Soghoian DZ, Kavanagh DG, Reynolds S, Norris B, Mordecai SK, Nguyen Q, Lai C, Kaufmann DE
Journal
PubMed ID25472703
Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single-cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human leukocytes. This technique can ... More
RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.
AuthorsYu M, Ting DT, Stott SL, Wittner BS, Ozsolak F, Paul S, Ciciliano JC, Smas ME, Winokur D, Gilman AJ, Ulman MJ, Xega K, Contino G, Alagesan B, Brannigan BW, Milos PM, Ryan DP, Sequist LV, Bardeesy N, Ramaswamy S, Toner M, Maheswaran S, Haber DA
JournalNature
PubMed ID22763454
Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. Although these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse ... More
Urinary Xist is a potential biomarker for membranous nephropathy.
AuthorsHuang YS, Hsieh HY, Shih HM, Sytwu HK, Wu CC
Journal
PubMed ID25157805
Membranous nephropathy (MN), a type of glomerular nephritis, is the most common cause of nephrotic syndrome in human adults. Changes in gene expression as a result of epigenetic dysregulation through long noncoding RNAs (lncRNAs) are increasingly being recognized as important factors in disease. Using an experimental MN mouse model, we ... More
Prion-mediated neurodegeneration is associated with early impairment of the ubiquitin-proteasome system.
AuthorsMcKinnon C, Goold R, Andre R, Devoy A, Ortega Z, Moonga J, Linehan JM, Brandner S, Lucas JJ, Collinge J, Tabrizi SJ
JournalActa Neuropathol
PubMed ID26646779
'Prion diseases are a group of fatal neurodegenerative disorders characterised by the accumulation of misfolded prion protein (PrP(Sc)) in the brain. The critical relationship between aberrant protein misfolding and neurotoxicity currently remains unclear. The accumulation of aggregation-prone proteins has been linked to impairment of the ubiquitin-proteasome system (UPS) in a ... More
Non-isotopic Method for In Situ LncRNA Visualization and Quantitation.
AuthorsMaqsodi B, Nikoloff C
JournalMethods Mol Biol
PubMed ID26721490
'In mammals and other eukaryotes, most of the genome is transcribed in a developmentally regulated manner to produce large numbers of long noncoding RNAs (lncRNAs). Genome-wide studies have identified thousands of lncRNAs lacking protein-coding capacity. RNA in situ hybridization technique is especially beneficial for the visualization of RNA (mRNA and ... More
Detection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes.
AuthorsBusse N, Paroni F, Richardson SJ, Laiho JE, Oikarinen M, Frisk G, Hyöty H, de Koning E, Morgan NG, Maedler K
JournalOncotarget
PubMed ID28147344
'Enteroviruses, specifically of the Coxsackie B virus family, have been implicated in triggering islet autoimmunity and type 1 diabetes, but their presence in pancreata of patients with diabetes has not been fully confirmed.To detect the presence of very low copies of the virus genome in tissue samples from T1D patients, ... More
Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models.
AuthorsZhang X, Yue L, Zhang Z, Yuan Z
JournalEmerg Microbes Infect
PubMed ID29116221
'While chronic hepatitis B remains a global public health problem, the detailed spatiotemporal dynamics of the key molecular events leading to the multiplication and egress of hepatitis B virus (HBV) are still largely unclear. Previously, we developed a chromogenic in situ hybridization assay for detection of HBV RNA, DNA and ... More
Methods to Quantify the NF-?B Pathway During Senescence.
AuthorsZhang L, Zhao J, Gurkar A, Niedernhofer LJ, Robbins PD
JournalMethods Mol Biol
PubMed ID30474851
'Nuclear factor ?B (NF-?B) is a family of transcription factors important for regulating innate and adaptive immunity, cellular proliferation, apoptosis and senescence. The NF-?B family is comprised of five subunits, RelA/p65, RelB, C-Rel, p50 (p105/NF-?B1), and p52 (p100/NF-?B2). NF-?B activity goes up with age in multiple tissues. The two subunits ... More
Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses Upon Cell-Cell Contact.
AuthorsColéon S, Assil S, Dreux M
JournalMethods Mol Biol
PubMed ID30593636
'Plasmacytoid dendritic cells (pDCs) constitute a unique DC subset specialized in rapid and massive secretion of cytokines, including type I interferon (i.e., IFNa and IFNß), known to be pivotal for both innate immunity and the onset of adaptive response. The production of type I IFNs by pDCs is primarily induced by the ... More
Innate immune response of human epidermal keratinocytes and dermal fibroblasts to in vitro incubation of Trichophyton benhamiae DSM 6916.
'Superficial cutaneous infection caused by the zoophilic dermatophyte Trichophyton benhamiae is often associated with a highly inflammatory immune response. As non-professional immune cells, epidermal keratinocytes and dermal fibroblasts contribute to the first line of defence by producing pro-inflammatory cytokines and antimicrobial peptides (AMP).' ... More
The IFITMs Inhibit Zika Virus Replication.
AuthorsSavidis G, Perreira JM, Portmann JM, Meraner P, Guo Z, Green S, Brass AL
JournalCell Rep
PubMed ID27268505
Zika virus has emerged as a severe health threat with a rapidly expanding range. The IFITM family of restriction factors inhibits the replication of a broad range of viruses, including the closely related flaviruses West Nile virus and dengue virus. Here, we show that IFITM1 and IFITM3 inhibit Zika virus ... More
Albumin expression distinguishes bile duct adenomas from metastatic adenocarcinoma.
AuthorsMoy AP, Arora K, Deshpande V
JournalHistopathology
PubMed ID26841202
Bile duct adenomas may be difficult to distinguish from metastatic carcinomas, particularly well-differentiated pancreatic ductal adenocarcinoma. Prior studies have evaluated the utility of various immunohistochemical markers, although these markers are notable for low sensitivity and/or specificity. The aim of this study was to investigate the utility of albumin and BRAFV600E ... More
Stem cell-derived hepatocytes: A novel model for hepatitis E virus replication.
AuthorsHelsen N, Debing Y, Paeshuyse J, Dallmeier K, Boon R, Coll M, Sancho-Bru P, Claes C, Neyts J, Verfaillie CM
JournalJ Hepatol
PubMed ID26626494
Yearly, approximately 20million people become infected with the hepatitis E virus (HEV) resulting in over 3million cases of acute hepatitis. Although HEV-mediated hepatitis is usually self-limiting, severe cases of fulminant hepatitis as well as chronic infections have been reported, resulting annually in an estimated 60,000 deaths. We studied whether pluripotent ... More
Fetal-type gastrointestinal adenocarcinoma: a morphologically distinct entity with unfavourable prognosis.
AuthorsArora K, Bal M, Shih A, Moy A, Zukerberg L, Brown I, Liu X, Kelly P, Oliva E, Mullen J, Ahn S, Kim KM, Deshpande V
JournalJ Clin Pathol
PubMed ID28814568
This multi-institutional study and a re-evaluation of the TCGA cohort explores the morphological spectrum, genetics and outcome of GI (gastrointestinal) hepatoid tumours, tumours expressing alpha-fetoprotein (AFP) and fetal-type (FT) GI adenocarcinomas. ... More
Long noncoding RNA DANCR promotes nasopharyngeal carcinoma progression by interacting with STAT3, enhancing IL-6/JAK1/STAT3 signaling.
AuthorsZhang X, Yang J, Bian Z, Shi D, Cao Z
JournalBiomed Pharmacother
PubMed ID30849642
Nasopharyngeal carcinoma (NPC) is the most common type of malignancy of the neck and head in Southeast Asia and North Africa. Long noncoding RNA (LncRNA) Differentiation antagonizing nonprotein coding RNA (DANCR) has been reported to exert oncogenic functions in various malignant tumors. However, whether DANCR is involved in NPC tumorgenesis ... More
Glass confers rhabdomeric photoreceptor identity in
AuthorsBernardo-Garcia FJ, Syed M, Jékely G, Sprecher SG
JournalEvodevo
PubMed ID30873275
Across metazoans, visual systems employ different types of photoreceptor neurons (PRs) to detect light. These include rhabdomeric PRs, which exist in distantly related phyla and possess an evolutionarily conserved phototransduction cascade. While the development of rhabdomeric PRs has been thoroughly studied in the fruit fly ... More
Non-coding RNA Neat1 and Abhd11os expressions are dysregulated in medium spiny neurons of Huntington disease model mice.
AuthorsPark H, Miyazaki H, Yamanaka T, Nukina N
JournalNeurosci Res
PubMed ID30391555
Huntington Disease (HD) is a neurodegenerative disorder caused by expanded CAG repeats in the exon1 of huntingtin gene (HTT). The mutant HTT affects the transcriptional profile of neurons by disrupting the activities of transcriptional machinery and alters expression of many genes. In this study, we identified dysregulated non-coding RNAs (ncRNAs) ... More
Quasimesenchymal phenotype predicts systemic metastasis in pancreatic ductal adenocarcinoma.
AuthorsMahadevan KK, Arora KS, Amzallag A, Williams E, Kulkarni AS, Fernandez-Del Castillo C, Lillemoe KD, Bardeesy N, Hong TS, Ferrone CR, Ting DT, Deshpande V
JournalMod Pathol
PubMed ID30683911
Metastasis following surgical resection is a leading cause of mortality in pancreatic ductal adenocarcinoma. Epithelial-mesenchymal transition is thought to play an important role in metastasis, although its clinical relevance in metastasis remains uncertain. We evaluated a panel of RNA in-situ hybridization probes for epithelial-mesenchymal transition-related genes expressed in circulating tumor ... More
A genetically encodable cell-type-specific protein synthesis inhibitor.
AuthorsHeumüller M, Glock C, Rangaraju V, Biever A, Schuman EM
JournalNat Methods
PubMed ID31308551
Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, ... More
Multiple cytokine-producing B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma and classic Hodgkin lymphoma with autoimmune hemolytic anemia.
AuthorsNakayama S, Morita Y, Espinoza JL, Rai S, Oyama Y, Taniguchi T, Miyake Y, Tanaka H, Matsumura I
JournalLeuk Lymphoma
PubMed ID31533519
RDI Calculator: An Analysis Tool to Assess RNA Distributions in Cells.
AuthorsStueland M, Wang T, Park HY, Mili S
JournalSci Rep
PubMed ID31164708
Localization of RNAs to various subcellular destinations has emerged as a widely used mechanism that regulates a large proportion of transcripts in polarized cells. A number of methodologies have been developed that allow detection and imaging of RNAs at single-molecule resolution. However, methodologies to quantitatively describe RNA distributions are limited. ... More
Combinatorial processing of bacterial and host-derived innate immune stimuli at the single-cell level.
AuthorsGutschow MV, Mason JC, Lane KM, Maayan I, Hughey JJ, Bajar BT, Amatya DN, Valle SD, Covert MW
JournalMol Biol Cell
PubMed ID30462580
During the course of a bacterial infection, cells are exposed simultaneously to a range of bacterial and host factors, which converge on the central transcription factor nuclear factor (NF)-?B. How do single cells integrate and process these converging stimuli? Here we tackle the question of how cells process combinatorial signals ... More
Comparison of CD34 Expression in Fibrous Reactive Hyperplasia and Healthy Oral Mucosa.
AuthorsNagasaki M, Sakaguchi W, Fuchida S, Kubota N, Saruta J, Suzuki K, Iwabuchi H, Kobayashi M, Ishii S, Nakamura A, Yamamoto Y, Tsukinoki K
JournalJ Oral Biosci
PubMed ID32007660
Fibrous reactive hyperplasia (FRH) is a common fibrous lesion in the oral cavity. The disease characteristics of FRH, including the expression patterns of CD34, which is a well-known fibroblast marker, have not been investigated in detail. Therefore, in this study, we aimed to investigate the characteristics of FRH compared to ... More
The bacterial RNA ligase RtcB accelerates the repair process of fragmented rRNA upon releasing the antibiotic stress.
AuthorsManwar MR, Shao C, Shi X, Wang J, Lin Q, Tong Y, Kang Y, Yu J
JournalSci China Life Sci
PubMed ID31250189
RtcB, a highly conserved RNA ligase, is found in all three domains of life, and demonstrated to be an essential tRNA splicing component in archaea and metazoans. However, the biological functions of RtcB in bacteria, where there is no splicing, remains to be clarified. We first performed bioinformatics analysis which ... More
LINC00461/miR-4478/E2F1 feedback loop promotes non-small cell lung cancer cell proliferation and migration.
AuthorsMeng Q, Liu M, Cheng R
JournalBiosci Rep
PubMed ID31934717
Non-small cell lung cancer (NSCLC) is a prevalent subtype of lung cancer, whose mortality is high. Long non-coding RNAs (lncRNAs) have caught rising attentions because of their intricate roles in regulating cancerization and cancer progression. Long intergenic non-protein coding RNA 461 (LINC00461) has recently shown oncogenic potential in several cancers, ... More