IPTG 溶液，开封即用

Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis了解更多信息

货号	数量
R1171	10 x 1.5 mL

货号 R1171

价格（CNY)

631.00

Ends: 31-Dec-2025

901.00

共减 270.00 (30%)

添加至购物车

10 x 1.5 mL

请求批量或定制报价

价格（CNY)

631.00

Ends: 31-Dec-2025

901.00

共减 270.00 (30%)

添加至购物车

Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

Applications

• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies
• Induction of gene expression under control of the lac promoter

Note
• For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media
• Store IPTG Solution at -20°C.

Related Products
IPTG, dioxane-free
IPTG Solution, ready-to-use

仅供科研使用。不可用于诊断程序。

化学名称或材料异丙基硫代半乳糖苷

适用于（应用）蛋白表达

产品类型IPTG 溶液

数量10 x 1.5 mL

最大浓度0.1 M

Unit SizeEach

常见问题解答 (FAQ)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.