A novel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment.
AuthorsFunamoto K, Zervantonakis IK, Liu Y, Ochs CJ, Kim C, Kamm RD,
JournalLab Chip
PubMed ID23023115
'Low oxygen tensions experienced in various pathological and physiological conditions are a major stimulus for angiogenesis. Hypoxic conditions play a critical role in regulating cellular behaviour including migration, proliferation and differentiation. This study introduces the use of a microfluidic device that allows for the control of oxygen tension for the ... More
Saikosaponin-d Enhances the Anticancer Potency of TNF-a via Overcoming Its Undesirable Response of Activating NF-Kappa B Signalling in Cancer Cells.
AuthorsWong VK, Zhang MM, Zhou H, Lam KY, Chan PL, Law CK, Yue PY, Liu L,
JournalEvid Based Complement Alternat Med
PubMed ID23573150
'Tumor necrosis factor-alpha (TNF- a ) was reported as anticancer therapy due to its cytotoxic effect against an array of tumor cells. However, its undesirable responses of TNF- a on activating NF- ? B signaling and pro-metastatic property limit its clinical application in treating cancers. Therefore, sensitizing agents capable of ... More
Aryl hydrocarbon receptor protects against bacterial infection by promoting macrophage survival and reactive oxygen species production.
AuthorsKimura A, Abe H, Tsuruta S, Chiba S, Fujii-Kuriyama Y, Sekiya T, Morita R, Yoshimura A,
Journal
PubMed ID24343818
'Aryl hydrocarbon receptor (AhR) is crucial for various immune responses. The relationship between AhR and infection with the intracellular bacteria Listeria monocytogenes (LM) is poorly understood. Here, we show that in response to LM infection, AhR is required for bacterial clearance by promoting macrophage survival and reactive oxygen species (ROS) ... More
Circulating fibrocytes stabilize blood vessels during angiogenesis in a paracrine manner.
AuthorsLi J, Tan H, Wang X, Li Y, Samuelson L, Li X, Cui C, Gerber DA,
Journal
PubMed ID24300950
'Accumulating evidence supports that circulating fibrocytes play important roles in angiogenesis. However, the specific role of fibrocytes in angiogenesis and the underlying mechanisms remain unclear. In this study, we found that fibrocytes stabilized newly formed blood vessels in a mouse wound-healing model by inhibiting angiogenesis during the proliferative phase and ... More
Characterizing natural hydrogel for reconstruction of three-dimensional lymphoid stromal network to model T-cell interactions.
AuthorsKim J, Wu B, Niedzielski SM, Hill MT, Coleman RM, Ono A, Shikanov A,
Journal
PubMed ID25649205
'Hydrogels have been used in regenerative medicine because they provide a three-dimensional environment similar to soft tissues, allow diffusion of nutrients, present critical biological signals, and degrade via endogenous enzymatic mechanisms. Herein, we developed in vitro system mimicking cell-cell and cell-matrix interactions in secondary lymphoid organs (SLOs). Existing in vitro ... More
Matrix identity and tractional forces influence indirect cardiac reprogramming.
AuthorsKong YP, Carrion B, Singh RK, Putnam AJ,
Journal
PubMed ID24326998
Heart regeneration through in vivo cardiac reprogramming has been demonstrated as a possible regenerative strategy. While it has been reported that cardiac reprogramming in vivo is more efficient than in vitro, the influence of the extracellular microenvironment on cardiac reprogramming remains incompletely understood. This understanding is necessary to improve the ... More
Melatonin induces apoptosis of colorectal cancer cells through HDAC4 nuclear import mediated by CaMKII inactivation.
AuthorsWei JY, Li WM, Zhou LL, Lu QN, He W,
Journal
PubMed ID25752481
Melatonin induces apoptosis in many different cancer cell lines, including colorectal cancer. However, the precise mechanisms involved remain largely unresolved. In this study, we provide evidence to reveal a new mechanism by which melatonin induces apoptosis of colorectal cancer LoVo cells. Melatonin at pharmacological concentrations significantly suppressed cell proliferation and ... More
Nitric oxide-mediated cytotoxic effect induced by zoledronic acid treatment on human gingival fibroblasts.
AuthorsDe Colli M, Zara S, di Giacomo V, Patruno A, Marconi GD, Gallorini M, Zizzari VL, Tetè G, Cataldi A,
Journal
PubMed ID25352469
The aim of our work was to evaluate the role of nitric oxide (NO) in the in vitro response of human gingival fibroblasts (HGFs) to 1, 5, 10, and 100 µM doses of zoledronic acid (ZA), a bisphosphonate largely used in the clinical practice and for which several adverse effects are ... More
Isolation of single mammalian cells from adherent cultures by fluidic force microscopy.
AuthorsGuillaume-Gentil O, Zambelli T, Vorholt JA,
Journal
PubMed ID24270585
The physical separation of individual cells from cell populations for single-cell analysis and proliferation is of wide interest in biology and medicine. Today, single-cell isolation is routinely applied to non-adherent cells, though its application to cells grown on a substrate remains challenging. In this report, a versatile approach for isolating ... More
Clostridium difficile Toxin B causes epithelial cell necrosis through an autoprocessing-independent mechanism.
Clostridium difficile is the most common cause of antibiotic-associated nosocomial infection in the United States. C. difficile secretes two homologous toxins, TcdA and TcdB, which are responsible for the symptoms of C. difficile associated disease. The mechanism of toxin action includes an autoprocessing event where a cysteine protease domain (CPD) ... More
Inhibition of proliferation, migration and proteolysis contribute to corticosterone-mediated inhibition of angiogenesis.
AuthorsShikatani EA, Trifonova A, Mandel ER, Liu ST, Roudier E, Krylova A, Szigiato A, Beaudry J, Riddell MC, Haas TL,
JournalPLoS One
PubMed ID23056375
The angiostatic nature of pharmacological doses of glucocorticoid steroids is well known. However, the consequences of pathophysiological elevation of endogenous glucocorticoids are not well established. In the current study, we hypothesized that the angiostatic effect of corticosterone, an endogenous glucocorticoid in rodents, occurs through multi-faceted alterations in skeletal muscle microvascular ... More
Magnesium and vitamin C supplementation attenuates steroid-associated osteonecrosis in a rat model.
Authors
JournalBiomaterials
PubMed ID32045781
Calcium phosphate nanoparticle-mediated transfection in 2D and 3D mono- and co-culture cell models.
AuthorsSokolova V, Rojas-Sánchez L, Bialas N, Schulze N, Epple M
JournalActa Biomater
PubMed ID30503560
'The transfer of nucleic acids into living cells, i.e. transfection, is a major technique in current molecular biology and medicine. As nucleic acids alone are not able to penetrate the cell membrane, an efficient carrier is needed. Calcium phosphate nanoparticles can serve as carrier due to their biocompatibility, biodegradability and ... More
AuthorsRehman SRU, Augustine R, Zahid AA, Ahmed R, Tariq M, Hasan A
JournalInt J Nanomedicine
PubMed ID31824154
'Non-healing or slow healing chronic wounds are among serious complications of diabetes that eventually result in amputation of limbs and increased morbidities and mortalities. Chronic diabetic wounds show reduced blood vessel formation (lack of angiogenesis), inadequate cell proliferation and poor cell migration near wounds. In this paper, we report the ... More
A network-centric approach to drugging TNF-induced NF-κB signaling.
Authors
JournalNat Commun
PubMed ID30808860
Ultrafast Near-Infrared Light-triggered Intracellular Uncaging to Probe Cell Signaling.
Authors
JournalAdv Funct Mater
PubMed ID29176940
Soft fibers with magnetoelasticity for wearable electronics.
Authors
JournalNat Commun
PubMed ID34799591
SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs.
Authors
JournalCell Res
PubMed ID32801357
Pharmacological targeting of MCL-1 promotes mitophagy and improves disease pathologies in an Alzheimer's disease mouse model.
Authors
JournalNat Commun
PubMed ID33184293
Layer-by-layer approach for a uniformed fabrication of a cell patterned vessel-like construct.
Authors
JournalBiofabrication
PubMed ID27906688
A genome-wide RNAi screen draws a genetic framework for transposon control and primary piRNA biogenesis in Drosophila.
Authors
JournalMol Cell
PubMed ID23665228
A nanofibrous encapsulation device for safe delivery of insulin-producing cells to treat type 1 diabetes.
Authors
JournalSci Transl Med
PubMed ID34078744
TRPV4 channel opening mediates pressure-induced pancreatitis initiated by Piezo1 activation.
Authors
JournalJ Clin Invest
PubMed ID31999644
Kupffer Cells Survive
AuthorsTweedell RE, Qi L, Sun Z, Dinglasan RR
JournalPathogens
PubMed ID30477234
The liver stage of the
HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas.
AuthorsYang M, Yang X, Wang S, Xu L, Ke S, Ding X, Sun W, Gao J
JournalAm J Transl Res
PubMed ID31934300
The goal of this study was to assess the role of high-mobility group box 1 (HMGB1)-induced endothelial cell (EC) pyroptosis in systemic inflammatory response syndrome (SIRS) following radiofrequency (RF) ablation of hepatic hemangiomas. We enrolled 76 patients with hepatic hemangioma who underwent RF ablation. Serum concentrations of HMGB1, interleukin (IL)-1ß, ... More
CuS-MnS
AuthorsChen W, Wang X, Zhao B, Zhang R, Xie Z, He Y, Chen A, Xie X, Yao K, Zhong M, Yuan M
JournalNanoscale
PubMed ID31264665
With a high incidence and high mortality rate, ovarian cancer presents a challenge for clinical practice. It is thus extremely urgent to investigate new diagnosis and therapy methods for the treatment of ovarian cancer. Ternary copper-based chalcogenide nanomaterials are attractive owing to their near infrared (NIR) response for cancer theranostic ... More