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查看更多产品信息 NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI) - FAQs (R37606)
3 个常见问题解答
Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
We do not have a direct alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538). However, we do have alternative primary and secondary antibodies, as well as reagents, that can be used for trilineage differentiation. Please note that we have not internally validated the use of all these reagents together.
We recommend the following primary antibodies:
Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)) (Cat. No. MA5-11547)
alpha-Fetoprotein Monoclonal Antibody (AFP3) (Cat. No. 14-6583-80)
beta-3 Tubulin Monoclonal Antibody (2G10) (Cat. No. MA1-118)
We recommend the following secondary antibodies:
Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 555 (Cat. No. A21137)
Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 594 (Cat. No. A21135)
https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21121
We recommend using the following reagents:
https://www.thermofisher.com/order/catalog/product/88-8824-00
NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606)
Blocker BSA (Cat. No. 37520)
DPBS (10X), no calcium, no magnesium (Cat. No. 14200075)
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.