ReadyProbes™ Cell Viability Imaging Kit, Blue/Green - FAQs

查看更多产品信息 ReadyProbes™ Cell Viability Imaging Kit, Blue/Green - FAQs (R37609)

4 个常见问题解答

我在 Countess II FL全自动细胞计数仪上能进行哪种荧光活性实验？

我们已经确认可在Countess II FL全自动细胞计数仪上使用下列试剂盒：

•LIVE/DEAD Viabilty/Cytoxicity试剂盒（货号L3224）含有钙黄绿素AM和溴乙啡锭二聚体
•ReadyProbes Cell Viability Imaging试剂盒，蓝/绿（货号R37609）
•ReadyProbes Cell Viability Imaging试剂盒，蓝/红（货号R37610）含有NucBlue Live/NucGreen Dead和NucBlue Live/propidium iodide

见此应用说明(https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf)中的详情。

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fluorescent viability assays can I use on the Countess II FL automated cell counter?

We have validated the following kits for use on the Countess II FL Automated Cell Counter:

LIVE/DEAD Viabilty/Cytoxicity Kit (Cat. No. L3224) containing calcein AM and ethidium homodimer-1
ReadyProbes Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610) containing NucBlue Live/NucGreen Dead and NucBlue Live/propidium iodide
See this Application Note for details - https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between NucBlue Live reagents stain and NucGreen Dead reagents stain?

The NucBlue Live reagent stains the nuclei of all cells, while NucGreen Dead reagent stains only the nuclei of dead cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.