ReadyProbes™ 细胞活力成像试剂盒（蓝色/红色）

引用和文献 (9)

Invitrogen™

ReadyProbes™ 细胞活力成像试剂盒（蓝色/红色）

ReadyProbes® 细胞活力成像试剂盒（蓝色/红色）是一种即用型检测试剂盒，可快速且轻松地测定细胞活力。只需向1 mL 细胞生长培养基中加入室温下的稳定 NucBlue® Live 试剂了解更多信息

货号	数量
R37610	1 kit

货号 R37610

价格（CNY)

2,583.00

Ends: 31-Dec-2025

3,479.00

共减 896.00 (26%)

添加至购物车

1 kit

价格（CNY)

2,583.00

Ends: 31-Dec-2025

3,479.00

共减 896.00 (26%)

添加至购物车

ReadyProbes® 细胞活力成像试剂盒（蓝色/红色）是一种即用型检测试剂盒，可快速且轻松地测定细胞活力。只需向1 mL 细胞生长培养基中加入室温下的稳定 NucBlue® Live 试剂 (Hoechst 33342) 和碘化丙啶各2滴，然后即可通过计数总细胞与死细胞来确定细胞活力。NucBlue® Live 试剂可对所有细胞的细胞核进行染色，并可使用标准 DAPI 滤光片进行检测。碘化丙啶仅对细胞膜完整性受损细胞的细胞核进行染色，并能使用标准 TRITC/RFP（橙色）滤光片组进行检测。本试剂盒适用于荧光显微镜、荧光微孔板读数仪和流式细胞分析。

NucBlue® Live 试剂： 可对所有细胞的细胞核进行染色；使用标准 DAPI 滤光片进行检测（最大激发/发射波长：360/460 nm）
碘化丙啶： 仅对细胞膜受损的死细胞的细胞核进行染色；使用标准 TRITC/RFP（橙色）滤光片组进行检测（最大激发/发射波长：535/617 nm）
请参阅其他 ReadyProbes® 细胞染色试剂
进一步了解其他细胞活力测定试剂盒

使用建议
•NucBlue® Live 试剂和碘化丙啶可直接加入完全生长培养基的细胞或兼容的缓冲液中。
• 在大多数情况下，2滴/mL 以及5至30分钟的孵育时间将使细胞核染上明亮颜色；然而，可能需要针对一些细胞类型、条件和应用进行优化。在此种情况下，只需简单地加入更多或更少的液滴，直至获得较佳染色强度。

仅供科研使用。不可用于诊断程序。

细胞类型哺乳动物细胞、真核细胞

描述ReadyProbes 细胞活力成像试剂盒（蓝色/红色）

检测方法荧光

染料类型3 x 2.5 mL NucBlue™ Live 试剂；3 x 2.5 mL 碘化丙啶

产品规格滴瓶

数量1 kit

运输条件室温

颜色蓝色、红色

发射460、617 nm

Excitation Wavelength Range360、535 nm

适用于（应用）测定细胞活力

适用于（设备）荧光显微镜

产品线ReadyProbes

产品类型细胞活力成像试剂盒

Unit SizeEach

内容与储存

在 2° 至 8°C 或室温下储存。

常见问题解答 (FAQ)

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (9)

引用和文献

Abstract

miR-204-5p and miR-3065-5p exert antitumor effects on melanoma cells.

Authors:Palkina N, Komina A, Aksenenko M, Moshev A, Savchenko A, Ruksha T

Journal:Oncol Lett

PubMed ID:29844810

'MicroRNA (miR)-204-5p was previously identified to be downregulated in melanoma compared with melanocytic nevi. This observation prompted a functional study on miR-204-5p and the newly-identified miR-3065-5p, two miRNAs suggested to be tumor-suppressive oncomiRs. Application of miR-204-5p mimics or inhibitors resulted in a decrease or increase, respectively, in melanoma cell proliferation ... More

Notch3 is involved in adipogenesis of human adipose-derived stromal/stem cells.

Authors:Sandel DA, Liu M, Ogbonnaya N, Newman JJ

Journal:Biochimie

PubMed ID:29709509

'Human adipose-derived stromal/stem cells (hASCs) have tremendous therapeutic potential and the ability to offer insight into human development and disease. Here we subject human ASCs to siRNA-mediated knockdown of Notch3 cultured under both self-renewing and adipogenic differentiation conditions. Self-renewal was monitored by assessing viability and proliferation rates through staining and ... More

Schrödinger's microbes: Tools for distinguishing the living from the dead in microbial ecosystems.

Authors:Emerson JB, Adams RI, Román CMB, Brooks B, Coil DA, Dahlhausen K, Ganz HH, Hartmann EM, Hsu T, Justice NB, Paulino-Lima IG, Luongo JC, Lymperopoulou DS, Gomez-Silvan C, Rothschild-Mancinelli B, Balk M, Huttenhower C, Nocker A, Vaishampayan P, Rothschild LJ,

Journal:Microbiome

PubMed ID:28810907

'While often obvious for macroscopic organisms, determining whether a microbe is dead or alive is fraught with complications. Fields such as microbial ecology, environmental health, and medical microbiology each determine how best to assess which members of the microbial community are alive, according to their respective scientific and/or regulatory needs. ... More

High-throughput chromatin accessibility profiling at single-cell resolution.

Authors:Mezger A, Klemm S, Mann I, Brower K, Mir A, Bostick M, Farmer A, Fordyce P, Linnarsson S, Greenleaf W

Journal:Nat Commun

PubMed ID:30194434

'Here we develop a high-throughput single-cell ATAC-seq (assay for transposition of accessible chromatin) method to measure physical access to DNA in whole cells. Our approach integrates fluorescence imaging and addressable reagent deposition across a massively parallel (5184) nano-well array, yielding a nearly 20-fold improvement in throughput (up to ~1800 cells/chip, ... More

Beneficial effects of mesenchymal stem cells on adult porcine cardiomyocytes in non-contact co-culture.

Authors:Miklíková M, Jarkovská D, Cedíková M, Švíglerová J, Kuncová J, Nalos L, Kubíková T, Liška V, Holubová M, Lysák D, Králícková M, Vištejnová L, Štengl M

Journal:Physiol Res

PubMed ID:30607969

'Mesenchymal stem cells (MSCs) have been reported to improve survival of cardiomyocytes (CMCs) and overall regeneration of cardiac tissue. Despite promising preclinical results, interactions of MSCs and CMCs, both direct and indirect, remain unclear. In this study, porcine bone marrow MSCs and freshly isolated porcine primary adult CMCs were used ... More

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