miR-204-5p and miR-3065-5p exert antitumor effects on melanoma cells.
AuthorsPalkina N, Komina A, Aksenenko M, Moshev A, Savchenko A, Ruksha T
JournalOncol Lett
PubMed ID29844810
'MicroRNA (miR)-204-5p was previously identified to be downregulated in melanoma compared with melanocytic nevi. This observation prompted a functional study on miR-204-5p and the newly-identified miR-3065-5p, two miRNAs suggested to be tumor-suppressive oncomiRs. Application of miR-204-5p mimics or inhibitors resulted in a decrease or increase, respectively, in melanoma cell proliferation ... More
Notch3 is involved in adipogenesis of human adipose-derived stromal/stem cells.
AuthorsSandel DA, Liu M, Ogbonnaya N, Newman JJ
JournalBiochimie
PubMed ID29709509
'Human adipose-derived stromal/stem cells (hASCs) have tremendous therapeutic potential and the ability to offer insight into human development and disease. Here we subject human ASCs to siRNA-mediated knockdown of Notch3 cultured under both self-renewing and adipogenic differentiation conditions. Self-renewal was monitored by assessing viability and proliferation rates through staining and ... More
Schrödinger's microbes: Tools for distinguishing the living from the dead in microbial ecosystems.
AuthorsEmerson JB, Adams RI, Román CMB, Brooks B, Coil DA, Dahlhausen K, Ganz HH, Hartmann EM, Hsu T, Justice NB, Paulino-Lima IG, Luongo JC, Lymperopoulou DS, Gomez-Silvan C, Rothschild-Mancinelli B, Balk M, Huttenhower C, Nocker A, Vaishampayan P, Rothschild LJ,
JournalMicrobiome
PubMed ID28810907
'While often obvious for macroscopic organisms, determining whether a microbe is dead or alive is fraught with complications. Fields such as microbial ecology, environmental health, and medical microbiology each determine how best to assess which members of the microbial community are alive, according to their respective scientific and/or regulatory needs. ... More
High-throughput chromatin accessibility profiling at single-cell resolution.
AuthorsMezger A, Klemm S, Mann I, Brower K, Mir A, Bostick M, Farmer A, Fordyce P, Linnarsson S, Greenleaf W
JournalNat Commun
PubMed ID30194434
'Here we develop a high-throughput single-cell ATAC-seq (assay for transposition of accessible chromatin) method to measure physical access to DNA in whole cells. Our approach integrates fluorescence imaging and addressable reagent deposition across a massively parallel (5184) nano-well array, yielding a nearly 20-fold improvement in throughput (up to ~1800 cells/chip, ... More
Beneficial effects of mesenchymal stem cells on adult porcine cardiomyocytes in non-contact co-culture.
AuthorsMiklíková M, Jarkovská D, Cedíková M, Švíglerová J, Kuncová J, Nalos L, Kubíková T, Liška V, Holubová M, Lysák D, Králícková M, Vištejnová L, Štengl M
JournalPhysiol Res
PubMed ID30607969
'Mesenchymal stem cells (MSCs) have been reported to improve survival of cardiomyocytes (CMCs) and overall regeneration of cardiac tissue. Despite promising preclinical results, interactions of MSCs and CMCs, both direct and indirect, remain unclear. In this study, porcine bone marrow MSCs and freshly isolated porcine primary adult CMCs were used ... More
Estrogen-activated MDM2 disrupts mammary tissue architecture through a p53-independent pathway.
AuthorsKundu N, Brekman A, Kim JY, Xiao G, Gao C, Bargonetti J,
JournalOncotarget
PubMed ID28615518
The Cancer Genome Atlas (TCGA) data indicate that high MDM2 expression correlates with all subtypes of breast cancer. Overexpression of MDM2 drives breast oncogenesis in the presence of wild-type or mutant p53 (mtp53). Importantly, estrogen-receptor positive (ER+) breast cancers overexpress MDM2 and estrogen mediates this expression. We previously demonstrated that ... More
In vitro study of doxorubicin-induced oxidative stress in spermatogonia and immature Sertoli cells.
AuthorsTremblay AR, Delbes G
JournalToxicol Appl Pharmacol
PubMed ID29660436
Pediatric chemotherapy treatments can impair long-term male fertility. Unfortunately, no fertility preservation solution is available for pre-pubertal boys. Studies suggest that doxorubicin, used against pediatric cancers, induces oxidative stress in the testis. However, the targeted testicular cell types remain unknown. The goal of this study was to determine whether doxorubicin ... More
Coordinated investigations into the interactions between biologically mimicking (biomimetic) material constructs and stem cells advance the potential for the regeneration and possible direct replacement of diseased cells and tissues. Any clinically relevant therapies will require the development and optimization of methods that mass produce fully functional cells and tissues. Despite ... More
Single-Cell Reconstruction of Progression Trajectory Reveals Intervention Principles in Pathological Cardiac Hypertrophy.
AuthorsRen Z, Yu P, Li D, Li Z, Liao Y, Wang Y, Zhou B, Wang L
JournalCirculation
PubMed ID32098504
Pressure overload-induced pathological cardiac hypertrophy is a common predecessor of heart failure, the latter of which remains a major cardiovascular disease with increasing incidence and mortality worldwide. Current therapeutics typically involve partially relieving the heart's workload after the onset of heart failure. Thus, more pathogenesis-, stage-, and cell type-specific treatment ... More