Autophagy is increased in cryptorchid testis resulting in abnormal spermatozoa.
AuthorsYefimova MG, Buschiazzo A, Burel A, Lavault MT, Pimentel C, Jouve G, Jaillard S, Jegou B, Bourmeyster N, Ravel C
Journal
PubMed ID31031333
'Autophagy is involved in spermatogenesis by regulating germ cell maturation. This catabolic process increases with hyperthermic conditions to prevent the accumulation of damaged organelles. Cryptorchidism is associated with impairment of germ cell maturation revealed by the presence of immature forms of sperm cells in ejaculates. The aim of the present ... More
GFP-complementation assay to detect functional CPP and protein delivery into living cells.
AuthorsMilech N, Longville BA, Cunningham PT, Scobie MN, Bogdawa HM, Winslow S, Anastasas M, Connor T, Ong F, Stone SR, Kerfoot M, Heinrich T, Kroeger KM, Tan YF, Hoffmann K, Thomas WR, Watt PM, Hopkins RM,
Journal
PubMed ID26671759
'Efficient cargo uptake is essential for cell-penetrating peptide (CPP) therapeutics, which deliver widely diverse cargoes by exploiting natural cell processes to penetrate the cell''s membranes. Yet most current CPP activity assays are hampered by limitations in assessing uptake, including confounding effects of conjugated fluorophores or ligands, indirect read-outs requiring secondary ... More
BET inhibitor OTX015 targets BRD2 and BRD4 and decreases c-MYC in acute leukemia cells.
AuthorsCoudé MM, Braun T, Berrou J, Dupont M, Bertrand S, Masse A, Raffoux E, Itzykson R, Delord M, Riveiro ME, Herait P, Baruchel A, Dombret H, Gardin C,
Journal
PubMed ID25989842
The bromodomain (BRD) and extraterminal (BET) proteins including BRD2, BRD3 and BRD4 have been identified as key targets for leukemia maintenance. A novel oral inhibitor of BRD2/3/4, the thienotriazolodiazepine compound OTX015, suitable for human use, is available. Here we report its biological effects in AML and ALL cell lines and ... More
Hypomyelinating leukodystrophy-associated missense mutation in HSPD1 blunts mitochondrial dynamics.
AuthorsMiyamoto Y, Eguchi T, Kawahara K, Hasegawa N, Nakamura K, Funakoshi-Tago M, Tanoue A, Tamura H, Yamauchi J,
Journal
PubMed ID25957474
Myelin-forming glial cells undergo dynamic morphological changes in order to produce mature myelin sheaths with multiple layers. In the central nervous system (CNS), oligodendrocytes differentiate to insulate neuronal axons with myelin sheaths. Myelin sheaths play a key role in homeostasis of the nervous system, but their related disorders lead not ... More
Age-dependent changes in clock neuron structural plasticity and excitability are associated with a decrease in circadian output behavior and sleep.
AuthorsCurran JA, Buhl E, Tsaneva-Atanasova K, Hodge JJL
JournalNeurobiol Aging
PubMed ID30825692
'Aging has significant effects on circadian behavior across a wide variety of species, but the underlying mechanisms are poorly understood. Previous work has demonstrated the age-dependent decline in behavioral output in the model organism Drosophila. We demonstrate that this age-dependent decline in circadian output is combined with changes in daily ... More
Enteric short-chain fatty acids promote proliferation of human neural progenitor cells.
'Short-chain fatty acids (SCFAs) are a group of fatty acids predominantly produced during the fermentation of dietary fibers by the gut anaerobic microbiota. SCFAs affect many host processes in health and disease. SCFAs play an important role in the ''gut-brain axis'', regulating central nervous system processes, for example, cell-cell interaction, ... More
A high-throughput liquid biopsy for rapid rare cell separation from large-volume samples.
AuthorsLiu Y, Li T, Xu M, Zhang W, Xiong Y, Nie L, Wang Q, Li H, Wang W
JournalLab Chip
PubMed ID30516210
'Liquid biopsy techniques for rare tumor cell separation from body fluids have shown enormous promise in cancer detection and prognosis monitoring. This work established a high-throughput liquid biopsy platform with a high recovery rate and a high cell viability based on a previously reported 2.5D micropore-arrayed filtration membrane. Thanks to ... More
Evaluation of Compound Activity in Primary Human Intestinal Organoids Using Gene Expression and Histology.
AuthorsMarchelletta R, Yu J, Moon C, Kim MM
JournalCurr Protoc Pharmacol
PubMed ID30920154
'Human intestinal organoids have enabled performance of functional epithelial studies and modeling of human diseases of the intestine. This unit describes 1) a method to isolate and culture crypts from human intestinal tissue, 2) use of combinatorial methods to expand stem cell-enriched spheroids and differentiate them into organoids composed of ... More
Role of Mitochondrial Dynamics in Neuronal Development: Mechanism for Wolfram Syndrome.
AuthorsCagalinec M, Liiv M, Hodurova Z, Hickey MA, Vaarmann A, Mandel M, Zeb A, Choubey V, Kuum M, Safiulina D, Vasar E, Veksler V, Kaasik A,
JournalPLoS Biol
PubMed ID27434582
'Deficiency of the protein Wolfram syndrome 1 (WFS1) is associated with multiple neurological and psychiatric abnormalities similar to those observed in pathologies showing alterations in mitochondrial dynamics. The aim of this study was to examine the hypothesis that WFS1 deficiency affects neuronal function via mitochondrial abnormalities. We show that down-regulation ... More
Biphasic responses of human vascular smooth muscle cells to magnesium ion.
AuthorsMa J, Zhao N, Zhu D,
JournalJ Biomed Mater Res A
PubMed ID26402437
Magnesium-based alloys are promising in biodegradable cardiovascular stent applications. The degradation products of magnesium stents may have significant impacts on the surrounding vascular cells. However, knowledge on the interactions between magnesium ion and vascular cells at the molecular and cellular levels is still largely missing. Vascular smooth muscle cell (SMC) ... More
AuthorsWei Z, Yan L, Chen Y, Bao C, Deng J, Deng J,
JournalMol Med Rep
PubMed ID27277156
Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves. For hundreds of years, Mangifera indica Linn. leaf has been used as an ingredient in numerous traditional Chinese medicine preparations for the treatment of bronchitis. However, the pharmacological mechanism of mangiferin in the treatment of ... More
Data supporting mitochondrial morphological changes by SPG13-associated HSPD1 mutants.
AuthorsMiyamoto Y, Megumi FT, Hasegawa N, Eguchi T, Tanoue A, Tamura H, Yamauchi J,
JournalData Brief
PubMed ID26900593
The data is related to the research article entitled
Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production.
AuthorsMillrine D, Tei M, Gemechu Y, Kishimoto T,
JournalProc Natl Acad Sci U S A
PubMed ID27601648
Immunomodulatory drugs (IMiDs) are a family of compounds derived from thalidomide. Binding of the IMiD molecule to the Lon protease Cereblon initiates the degradation of substrates via the ubiquitin proteasome pathway. Here, we show that Cereblon forms a complex with Rabex-5, a regulator of immune homeostasis. Treatment with lenalidomide prevented ... More
Fyn/heterogeneous nuclear ribonucleoprotein E1 signaling regulates pancreatic cancer metastasis by affecting the alternative splicing of integrin ß1.
AuthorsJiang P, Li Z, Tian F, Li X, Yang J
JournalInt J Oncol
PubMed ID28560430
Pancreatic cancer is characterized by a dense desmoplastic reaction in which extracellular matrix proteins accumulate and surround tumor cells. Integrins and their related signaling molecules are associated with progression of pancreatic cancer. In the present study, the association between the metastasis of pancreatic cancer and the expression of hnRNP E1 ... More
Sleep deprivation regulates availability of PrP
Authorsda Luz MHM, Pino JMV, Santos TG, Antunes HKM, Martins VR, de Souza AAL, Torquato RJS, Lee KS
JournalJ Neurochem
PubMed ID31950499
PrP
Human mesenchymal stem cell (hMSC) differentiation towards cardiac cells using a new microbioanalytical method.
AuthorsSokolowska P, Zukowski K, Lasocka I, Szulc-Dabrowska L, Jastrzebska E
JournalAnalyst
PubMed ID32133460
Stem cells (SCs) are more and more often applied in tissue engineering and cell therapies, e.g. in regenerative medicine. Standard methods of SC differentiation are time consuming and ineffective. Therefore, new bioanalytical methods (i.e. Lab-on-a-Chip systems) are develop to improve such type of studies. Although, microtechnology is a rapidly growing ... More