Protein conformational disorders, which include certain types of retinitis pigmentosa, are a set of inherited human diseases in which mutant proteins are misfolded and often aggregated. Many opsin mutants associated with retinitis pigmentosa, the most common being P23H, are misfolded and retained within the cell. Here, we describe a pharmacological ... More
Impairment of MAD2B-PRCC interaction in mitotic checkpoint defective t(X;1)-positive renal cell carcinomas.
Authors Weterman M A; van Groningen J J; Tertoolen L; van Kessel A G;
JournalProc Natl Acad Sci U S A
PubMed ID11717438
'The papillary renal cell carcinoma (RCC)-associated (X;1)(p11;q21) translocation fuses the genes PRCC and TFE3 and leads to cancer by an unknown molecular mechanism. We here demonstrate that the mitotic checkpoint protein MAD2B interacts with PRCC. The PRCCTFE3 fusion protein retains the MAD2B interaction domain, but this interaction is impaired. In ... More
The expression level of the voltage-dependent anion channel controls life and death of the cell.
AuthorsAbu-Hamad S, Sivan S, Shoshan-Barmatz V,
JournalProc Natl Acad Sci U S A
PubMed ID16585511
Mitochondria not only generate cellular energy, but also act as the point for cellular decisions leading to apoptosis. The voltage-dependent anion channel (VDAC), as a major mitochondrial outer-membrane transporter, has an important role in energy production by controlling metabolite traffic and is also recognized as a key protein in mitochondria-mediated ... More
Cell Cycle Regulation and p53 Activation by Protein Phosphatase 2Calpha.
AuthorsOfek P, Ben-Meir D, Kariv-Inbal Z, Oren M, Lavi S,
JournalJ Biol Chem
PubMed ID12514180
Protein phosphatase 2C (PP2C) dephosphorylates a broad range of substrates, regulating stress response and growth-related pathways in both prokaryotes and eukaryotes. We now demonstrate that PP2Calpha, a major mammalian isoform, inhibits cell growth and activates the p53 pathway. In 293 cell clones, in which PP2Calpha expression is regulated by a ... More
Validated zinc finger protein designs for all 16 GNN DNA triplet targets.
Authors Liu Qiang; Xia ZhenQin; Case Casey C;
JournalJ Biol Chem
PubMed ID11726671
The Cys(2)-His(2)-type zinc finger DNA-binding proteins can be engineered to bind specifically to many different DNA sequences. A single zinc finger typically binds to a 3-4-base pair DNA subsite. One strategy for design is to identify highly specific fingers that recognize each of the 64 possible DNA triplets. We started ... More
Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells.
The inducible T-REx system and other inducible expression systems have been developed in order to control the expression levels of recombinant protein in mammalian cells. In order to study the effects of heterologous protein expression on mammalian host behavior, the gene for recombinant Human transferrin (hTf) was integrated into HEK-293 ... More