Directed cell growth on protein-functionalized hydrogel surfaces.
AuthorsHynd MR, Frampton JP, Dowell-Mesfin N, Turner JN, Shain W
JournalJ Neurosci Methods
PubMed ID17368788
'Biochemical surface modification has been used to direct cell attachment and growth on a biocompatible gel surface. Acrylamide-based hydrogels were photo-polymerized in the presence of an acroyl-streptavidin monomer to create planar, functionalized surfaces capable of binding biotin-labelled proteins. Soft protein lithography (microcontact printing) of proteins was used to transfer the ... More
Immobilization of acrylamide-modified oligonucleotides by co-polymerization.
AuthorsRehman FN, Audeh M, Abrams ES, Hammond PW, Kenney M, Boles TC
JournalNucleic Acids Res
PubMed ID9862993
A flexible chemistry for solid phase attachment of oligonucleotides is described. Oligonucleotides bearing 5'-terminal acrylamide modifications efficiently co-polymerize with acrylamide monomers to form thermally stable DNA-containing polyacrylamide co-polymers. Co-polymerization attachment is specific for the terminal acrylamide group. Stable probe-containing layers are easily fabricated on supports bearing exposed acrylic groups, including ... More
Calcium channel and glutamate receptor activities regulate actin organization in salamander retinal neurons.
AuthorsCristofanilli M, Akopian A,
JournalJ Physiol
PubMed ID16777935
Intracellular Ca2+ regulates a variety of neuronal functions, including neurotransmitter release, protein phosphorylation, gene expression and synaptic plasticity. In a variety of cell types, including neurons, Ca2+ is involved in actin reorganization, resulting in either actin polymerization or depolymerization. Very little, however, is known about the relationship between Ca2+ and ... More
A streptavidin surface on planar glass substrates for the detection of biomolecular interaction.
AuthorsBirkert O, Haake HM, Schütz A, Mack J, Brecht A, Jung G, Gauglitz G
JournalAnal Biochem
PubMed ID10873274
Based on the requirements of biomolecular interaction analysis on direct optical transducers, a streptavidin surface is examined. A general protocol was developed allowing the immobilization of biotinylated compounds using the rife biotin-streptavidin system. This type of surface modification can be applied to all biosensors using glass surfaces as sensor devices. ... More