Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.
AuthorsLee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,
JournalClin Cancer Res
PubMed ID18559604
'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More
Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.
AuthorsGaedicke S, Braun F, Prasad S, Machein M, Firat E, Hettich M, Gudihal R, Zhu X, Klingner K, Schüler J, Herold-Mende CC, Grosu AL, Behe M, Weber W, Mäcke H, Niedermann G,
Journal
PubMed ID24469819
'A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection ... More
Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.
AuthorsKarlsen TV, McCormack E, Mujic M, Tenstad O, Wiig H,
JournalAm J Physiol Heart Circ Physiol
PubMed ID22101523
'There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and ... More
Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
AuthorsMcCormack E, Mujic M, Osdal T, Bruserud Ø, Gjertsen BT,
JournalBlood
PubMed ID23243270
Antibodies play a fundamental role in diagnostic immunophenotyping of leukemias and in cell-targeting therapy. However, this versatility is not reflected in imaging diagnostics. In the present study, we labeled anti–human mAbs monochromatically against selected human myeloid markers expressed on acute myeloid leukemia (AML) cells, all with the same near-infrared fluorochrome. ... More
Influenza virus aerosol exposure and analytical system for ferrets.
AuthorsGustin KM, Belser JA, Wadford DA, Pearce MB, Katz JM, Tumpey TM, Maines TR,
JournalProc Natl Acad Sci U S A
PubMed ID21536880
Understanding the transmission ability of newly emerging influenza viruses is central to the development of public health preparedness and prevention strategies. Animals are used to model influenza virus infection and transmission, but the routinely used intranasal inoculation of a liquid virus suspension does not reflect natural infection. We report the ... More