SlowFade™ Gold 抗淬灭封片剂（含 DAPI）
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SlowFade™ Gold 抗淬灭封片剂（含 DAPI）
引用和文献 (8)
Invitrogen™

SlowFade™ Gold 抗淬灭封片剂（含 DAPI）

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SlowFade Gold 抗淬灭封片剂是一种直接用于显微玻片上荧光标记的细胞或组织样品的液态封片剂。其所含的化学组分可在荧光显微镜实验过程中避免荧光染料淬灭（光漂白）。SlowFade Gold 抗淬灭封片剂基于甘油，无需混合或固化，因此非常适合即时观察样品。即用型—只需滴入样品了解更多信息
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货号数量
S3693810 mL
S369395 x 2 mL
S369422 mL
货号 S36938
价格（CNY)
1,810.00
飞享价
Ends: 31-Dec-2025
2,438.00
共减 628.00 (26%)
Each
添加至购物车
数量:
10 mL
价格（CNY)
1,810.00
飞享价
Ends: 31-Dec-2025
2,438.00
共减 628.00 (26%)
Each
添加至购物车
SlowFade Gold 抗淬灭封片剂是一种直接用于显微玻片上荧光标记的细胞或组织样品的液态封片剂。其所含的化学组分可在荧光显微镜实验过程中避免荧光染料淬灭（光漂白）。SlowFade Gold 抗淬灭封片剂基于甘油，无需混合或固化，因此非常适合即时观察样品。即用型—只需滴入样品、盖上玻片，随即可成像。其可提供含/不含 DAPI 核染色剂的选择。

主要性质：
•可在成像过程中避免染料淬灭
• 即用型液体，非常适合即时观察样品
• 封片后的样品可稳定保存数周
• 保持荧光信号强度—很少或完全不会淬灭
• 非常适合 Alexa Fluor 染料

根据您的荧光成像需求选择最适合的抗淬灭封片或光学透明化试剂 ›

SlowFade Gold 抗淬灭封片剂不建议用于封片含荧光蛋白（如 GFP）的样品。为对培养细胞或薄组织切片中的荧光蛋白和荧光染料提供出色的抗淬灭保护性能，建议使用 SlowFade Diamond 抗淬灭封片剂。为在 0–500 μm 焦深下实现高分辨率或成像较厚的组织或 3D 细胞培养物，可尝试 SlowFade Glass 抗淬灭封片剂。

根据您的实验需求选择最适合的 SlowFade 抗淬灭试剂›

仅供科研使用。不可用于诊断程序。
规格
环保功能危害更小、可持续包装
标签或染料DAPI
产品线SlowFade
数量10 mL
运输条件室温
适用于（设备）显微镜
形式Liquid
产品类型抗淬灭固定剂
溶液类型抗褪色溶液
Unit SizeEach
内容与储存
建议在室温下储存、但也可进行冷冻储存（-5 至 -30°C ）。避光。

常见问题解答 (FAQ)

DAPI和Hoechst 染料非常相似，该如何选用？

DAPI是一种非常普通的蓝色核复染荧光染料，能对固定和通透的细胞和组织的细胞核进行非常明亮的标记。遗憾的是，人们普遍认为它是介于半通透性到非通透性之间的染色剂，对活细胞的染色效果也不一致。Hoechst 33342染料是细胞通透性染料，与DAPI染色有相似的染色结合机制和荧光颜色；它是活细胞成像的首选，且对固定细胞的标记效果和DAPI一样好。

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (8)

引用和文献
Abstract
Alkylation damage causes MMR-dependent chromosomal instability in vertebrate embryos.
Authors:Feitsma H, Akay A, Cuppen E,
Journal:Nucleic Acids Res
PubMed ID:18522974
'S(N)1-type alkylating agents, like N-methyl-N-nitrosourea (MNU) and N-ethyl-N-nitrosourea (ENU), are potent mutagens. Exposure to alkylating agents gives rise to O(6)-alkylguanine, a modified base that is recognized by DNA mismatch repair (MMR) proteins but is not repairable, resulting in replication fork stalling and cell death. We used a somatic mutation detection ... More
Identification of a novel signal in the cytoplasmic tail of the Na+:HCO3- cotransporter NBC1 that mediates basolateral targeting.
Authors:Li HC, Li EY, Neumeier L, Conforti L, Soleimani M,
Journal:Am J Physiol Renal Physiol
PubMed ID:17182531
'The Na(+):HCO(3)(-) cotransporter NBC1 (SLC4A4, variant A, kidney specific) is located exclusively on the basolateral membrane of epithelial cells, implying that this molecule has acquired specific signals for targeting to the basolateral membrane. A motif with the sequence QQPFLS (positions 1010-1015) in the cytoplasmic tail of NBC1 was recently demonstrated ... More
Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.
Authors:Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K
Journal:Am J Physiol Renal Physiol
PubMed ID:16159901
'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More
Identification and characterization of small molecules that inhibit intracellular toxin transport.
Authors:Saenz JB, Doggett TA, Haslam DB
Journal:Infect Immun
PubMed ID:17576758
'Shiga toxin (Stx), cholera toxin (Ctx), and the plant toxin ricin are among several toxins that reach their intracellular destinations via a complex route. Following endocytosis, these toxins travel in a retrograde direction through the endosomal system to the trans-Golgi network, Golgi apparatus, and endoplasmic reticulum (ER). There the toxins ... More
Enhanced innate antiviral gene expression, IFN-a, and cytolytic responses are predictive of mucosal immune recovery during simian immunodeficiency virus infection.
Authors:Verhoeven D, George MD, Hu W, Dang AT, Smit-McBride Z, Reay E, Macal M, Fenton A, Sankaran-Walters S, Dandekar S,
Journal:
PubMed ID:24610016
The mucosa that lines the respiratory and gastrointestinal (GI) tracts is an important portal of entry for pathogens and provides the first line of innate immune defense against infections. Although an abundance of memory CD4(+) T cells at mucosal sites render them highly susceptible to HIV infection, the gut and ... More
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