'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More
Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells.
AuthorsHuang H, Starodub O, McIntosh A, Atshaves BP, Woldegiorgis G, Kier AB, Schroeder F
JournalBiochemistry
PubMed ID14992586
'Although it is hypothesized that long-chain fatty acyl CoAs (LCFA-CoAs) and long-chain fatty acids (LCFAs) regulate transcription in the nucleus, little is known regarding factors that determine the distribution of these ligands to nuclei of living cells. Immunofluorescence colocalization showed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well ... More
Confocal laser scanning microscopy of urinary bladder after intravesical instillation of a fluorescent dye.
AuthorsKoenig F, Knittel J, Schnieder L, George M, Lein M, Schnorr D
JournalUrology
PubMed ID12837458
'OBJECTIVES: To assess the potential of confocal laser scanning microscopy for imaging of the urinary bladder after intravesical instillation of a fluorescent dye. METHODS: The study was performed on the bladder of male Copenhagen rats. For confocal fluorescence microscopy (CFM), a standard confocal laser scanning microscope (Zeiss LSM 410) was ... More
Induction of DNA damage response by the supravital probes of nucleic acids.
'The aim of this study was to assess the potential DNA damage response (DDR) to four supravitally used biomarkers Hoechst 33342 (Ho 42), DRAQ5, DyeCycle Violet (DCV), and SYTO 17. A549 cells were exposed to these biomarkers at concentrations generally applied to live cells and their effect on histone H2AX ... More
Fiber cell denucleation in the primate lens.
AuthorsBassnett S
JournalInvest Ophthalmol Vis Sci
PubMed ID9286256
'PURPOSE: To determine the morphologic and biochemical events preceding the breakdown of fiber cell nuclei in the primate lens. METHODS: Monkey lens slices were labeled with fluorescent probes and optically sectioned using a confocal microscope. The distribution of nuclear histones was visualized by immunofluorescence. DNA and cellular membranes were imaged ... More
Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions.
AuthorsWojcik K, Dobrucki JW,
JournalCytometry A
PubMed ID18459157
'DNA-binding dyes are useful in contrasting cell nuclei and chromatin for live cell fluorescence microscopy; however, they may interfere with nuclear and DNA structure and function. We investigated the influence exerted by two DNA dyes on chromatin and nuclear structure, as well as histone-DNA interactions in live HeLa cells. A ... More
Assessment of the effects of gramicidin, formaldehyde, and surfactants on Escherichia coli by flow cytometry using nucleic acid and membrane potential dyes.
AuthorsComas J, Vives-Rego J
JournalCytometry
PubMed ID9298812
'Two membrane potential sensitive dyes (Rhodamine 123 and bis-oxonol) and three nucleic acid dyes (propidium iodide, SYTO-13, and SYTO-17) were used to assess the effect of surfactants on Escherichia coli. The ability of E. coli to be stained by these probes was validated at different physiological states. Propidium iodide was ... More
Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.
AuthorsDay JP, Kell DB, Griffith GW
JournalAppl Environ Microbiol
PubMed ID11772606
'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria.
AuthorsGuy R, Liu P, Pennefather P, Crandall I,
JournalMalar J
PubMed ID17617912
'BACKGROUND: Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium-parasitized culture and blood smears for both bright field and fluorescence was developed and its ability to improve ... More
Mixed infection of human U-937 cells by two different species of Leishmania.
AuthorsAbdullah SM, Flath B, Presber W
JournalAm J Trop Med Hyg
PubMed ID9715931
Mixed infections by different Leishmania species could explain differences in the clinical course of these infections. Moreover, mixed infections of the same macrophage could be the basis for parasite recombination. We stained three strains of Leishmania (L. mexicana amazonensis, L. donovani DD8, and L. infantum D2, respectively) with different fluorescent ... More
Functional analysis of the transmembrane domain in paramyxovirus F protein-mediated membrane fusion.
AuthorsBissonnette ML, Donald JE, DeGrado WF, Jardetzky TS, Lamb RA,
JournalJ Mol Biol
PubMed ID19121325
To enter cells, enveloped viruses use fusion-mediating glycoproteins to facilitate the merger of the viral and host cell membranes. These glycoproteins undergo large-scale irreversible refolding during membrane fusion. The paramyxovirus parainfluenza virus 5 mediates membrane merger through its fusion protein (F). The transmembrane (TM) domains of viral fusion proteins are ... More
SYTO probes in the cytometry of tumor cell death.
AuthorsWlodkowic D, Skommer J, Darzynkiewicz Z,
JournalCytometry A
PubMed ID18260152
Apoptosis is a complex and finely controlled cell death process of great relevance in tissue homeostasis and pathogenesis. The majority of classical apoptotic features can be examined by flow as well as image cytometry. Therefore, cytometry has been used as a technology of choice in studies of tumor cell demise. ... More
A multicolor, no-lyse no-wash assay for the absolute counting of CD34+ cells by flow cytometry.
AuthorsAlvarez-Larran A, Jover L, Marin P, Petriz J
JournalCytometry
PubMed ID12360574
BACKGROUND: We previously developed a method for counting CD34(+) cells in unlysed whole blood. This method was applied to normal human bone marrow, peripheral blood after mobilization of progenitor cells, leukapheresis products, and cord blood and was validated with two different lyse-no wash methods. However, the main advantage that we ... More
Flow cytometric analysis of apoptotic subpopulations with a combination of annexin V-FITC, propidium iodide, and SYTO 17.
AuthorsEray M, Mättö M, Kaartinen M, Andersson L, Pelkonen J
JournalCytometry
PubMed ID11169578
BACKGROUND: We have previously characterized apoptotic cell death induced in a follicular lymphoma cell line, HF-1, after triggering via the B-cell receptor (BCR) or treatment with Ca(2+) Ionophore A23187. We analyzed the kinetics of apoptosis induced by these two treatments, as two alternative models of classical apoptosis, by flow cytometry ... More
Transgenic cystic fibrosis mice exhibit reduced early clearance of Pseudomonas aeruginosa from the respiratory tract.
AuthorsSchroeder TH, Reiniger N, Meluleni G, Grout M, Coleman FT, Pier GB
JournalJ Immunol
PubMed ID11390493
The cystic fibrosis (CF) transmembrane conductance regulator (CFTR) has been proposed to be an epithelial cell receptor for Pseudomonas aeruginosa involved in bacterial internalization and clearance from the lung. We evaluated the role of CFTR in clearing P. aeruginosa from the respiratory tract using transgenic CF mice that carried either ... More
The glycosphingolipid globotriaosylceramide in the metastatic transformation of colon cancer.
AuthorsKovbasnjuk O, Mourtazina R, Baibakov B, Wang T, Elowsky C, Choti MA, Kane A, Donowitz M
JournalProc Natl Acad Sci U S A
PubMed ID16365318
The most devastating aspect of cancer is the emergence of metastases. Thus, identification of potentially metastatic cells among a tumor cell population and the underlying molecular changes that switch cells to a metastatic state are among the most important issues in cancer biology. Here we show that, although normal human ... More
Variable selection and multivariate methods for the identification of microorganisms by flow cytometry.
AuthorsDavey HM, Jones A, Shaw AD, Kell DB
JournalCytometry
PubMed ID10554172
BACKGROUND: When exploited fully, flow cytometry can be used to provide multiparametric data for each cell in the sample of interest. While this makes flow cytometry a powerful technique for discriminating between different cell types, the data can be difficult to interpret. Traditionally, dual-parameter plots are used to visualize flow ... More
LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells.
AuthorsMaertens G, Cherepanov P, Pluymers W, Busschots K, De Clercq E, Debyser Z, Engelborghs Y
JournalJ Biol Chem
PubMed ID12796494
We have reported that human immunodeficiency virus type 1 (HIV-1) integrase (IN) forms a specific nuclear complex with human lens epithelium-derived growth factor/transcription co-activator p75 (LEDGF/p75) protein. We now studied the IN-LEDGF/p75 interaction and nuclear import of IN in living cells using fusions of IN and LEDGF/p75 with enhanced green ... More
Histopathological assessment of laterality defects in zebrafish development.
Authors
JournalAnim Cells Syst (Seoul)
PubMed ID34262656
Outer Radial Glia-like Cancer Stem Cells Contribute to Heterogeneity of Glioblastoma.
Authors
JournalCell Stem Cell
PubMed ID31901251
LDB1 Enforces Stability on Direct and Indirect Oncoprotein Partners in Leukemia.
Authors
JournalMol Cell Biol
PubMed ID32229578
Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML.