iBind™ Flex Solution Kit

The iBind Flex Solution Kit is intended for use with the iBind Flex Western Device, which is an automated western-processingRead more

Catalog Number	Quantity	Product Type
SLF2020	1 kit	Flex Solution Kit

Catalog number SLF2020

Price (CNY)

1,855.00

1 kit

Add to cart

Quantity:

1 kit

Product Type:

Flex Solution Kit

Price (CNY)

1,855.00

1 kit

Add to cart

The iBind Flex Solution Kit is intended for use with the iBind Flex Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. The iBind solutions act as the blocking, washing, and dilution solutions in the iBind immunodetection process and have been optimized for ideal viscosity. Each solution is released from the iBind Flex device wells to the iBind Flex Card through sequential lateral flow (SLF); the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Learn more about the iBind western systems ›

The iBind Flex Solutions Kit, together with iBind Flex Western Device and iBind Flex Cards, bring automation and simplicity to the western protocol. Compared to manual methods, the iBind Flex western system requires less primary antibody, enables reproducible results, and is compatible with downstream detection protocols. Each iBind Flex Solution Kit contains sufficient solution for 10 midi or 20 mini blots. For fluorescent applications use the iBind Flex Fluorescent Detection (FD) Solution Kit.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)iBind Flex Western Device

Kit ContentsiBind™ Flex 5X buffer, iBind™ Flex 100X additive

No. of Reactions10 midi blots or 20 mini blots

Quantity1 kit

Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice

Detection MethodColorimetric, Chromogenic, Chemiluminescence

Membrane CompatibilityNitrocellulose, PVDF

Product LineiBind™

Product TypeFlex Solution Kit

Unit Size1 kit

Contents & Storage

• iBind Flex 5X Buffer
• iBind Flex 100X Additive

Store at 2–8°C.

Frequently asked questions (FAQs)

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBind™ Flex Solution Kit, 1 kit FAQs