Why should I use Thermo Scientific white plastics in qPCR?
White plates and tubes reflect significantly more signal back to the qPCR instrument's detector than traditional clear plates. The improved signal reflection ensures that even the lowest levels of fluorescence are detected. White well walls also prevent signal from passing through to the cycler block where it can be inconsistently reflected or absorbed. This keeps variations in the cycler block from affecting qPCR data. Using Thermo Scientific white PCR plastics increases sensitivity and reduces variability within the qPCR assay.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Will ultra thin wall (UTW) vessels automatically speed up reactions on my conventional thermal cycler?
To realize the time savings of the UTW plastics on conventional thermal cyclers, optimization of the protocol is required. This typically involves reducing hold times and changing the actual hold temperatures.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Are ultra thin wall (UTW) vessels prone to break more often because of their thinner walls?
When used as instructed, these vessels are remarkably robust and can handle typical laboratory conditions, without fracturing or resulting in loss of volume. It is recommended that, when applying caps, a proper tray be used to protect the bottom of the tubes from excessive force that is typically used.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Can I use lower volumes in my 96-well Piko PCR Plate than I could in a conventional 96-well microplate?
Yes. Typically, you should be able to use less than half the volume that you currently use on your conventional cycler and achieve equivalent or better results.