FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide)

FM™ 5-95 (<i>N</i>-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide)

Citations & References (14)

Invitrogen™

FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide)

The increasing number of successful applications for our FM dyes has prompted us to synthesize FM 5-95, a slightly lessRead more

Catalog Number	Quantity
T23360	1 mg

Catalog number T23360

Price (CNY)

5,483.00

Online Exclusive

Ends: 31-Dec-2025

7,434.00

Save 1,951.00 (26%)

Add to cart

Quantity:

Price (CNY)

5,483.00

Online Exclusive

Ends: 31-Dec-2025

7,434.00

Save 1,951.00 (26%)

Add to cart

The increasing number of successful applications for our FM dyes has prompted us to synthesize FM 5-95, a slightly less lipophilic analog of FM 4-64 (T-3166, T-13320) with essentially identical spectroscopic properties.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorRed

Detection MethodFluorescence

For Use With (Equipment)Fluorescence Microscope

Product LineFM

Quantity1 mg

Shipping ConditionRoom Temperature

Label TypeFluorescent Dye

Product TypeDye

SubCellular LocalizationPlasma Membrane

Unit Size1 mg

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (14)

Citations & References

Abstract

Glutamate receptor dynamics organizing synapse formation in vivo.

Authors:Rasse TM, Fouquet W, Schmid A, Kittel RJ, Mertel S, Sigrist CB, Schmidt M, Guzman A, Merino C, Qin G, Quentin C, Madeo FF, Heckmann M, Sigrist SJ

Journal:Nat Neurosci

PubMed ID:16136672

'Insight into how glutamatergic synapses form in vivo is important for understanding developmental and experience-triggered changes of excitatory circuits. Here, we imaged postsynaptic densities (PSDs) expressing a functional, GFP-tagged glutamate receptor subunit (GluR-IIA(GFP)) at neuromuscular junctions of Drosophila melanogaster larvae for several days in vivo. New PSDs, associated with functional ... More

Fluorescence ratio imaging microscopy shows decreased access of vancomycin to cell wall synthetic sites in vancomycin-resistant Staphylococcus aureus.

Authors:Pereira PM, Filipe SR, Tomasz A, Pinho MG,

Journal:Antimicrob Agents Chemother

PubMed ID:17646417

'A new method of fluorescence ratio imaging microscopy was used to compare the in vivo binding capacity and the access of a fluorescent derivative of vancomycin to the cell wall synthetic sites in isogenic pairs of vancomycin-susceptible and -resistant laboratory mutants and vancomycin-intermediate and -susceptible clinical isolates of Staphylococcus aureus. ... More

Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity.

Authors:Lemke EA, Klingauf J

Journal:J Neurosci

PubMed ID:16306416

'How synaptic vesicles move within central nervous synapses to their docking sites at the plasma membrane is widely discussed in synaptic physiology. This question is especially difficult to investigate in the small hippocampal boutons, which themselves can slowly move during observation in primary cell culture. Here, we describe a single ... More

SynCAM, a synaptic adhesion molecule that drives synapse assembly.

Authors:Biederer T, Sara Y, Mozhayeva M, Atasoy D, Liu X, Kavalali ET, Südhof TC

Journal:Science

PubMed ID:12202822

'Synapses, the junctions between nerve cells through which they communicate, are formed by the coordinated assembly and tight attachment of pre- and postsynaptic specializations. We now show that SynCAM is a brain-specific, immunoglobulin domain-containing protein that binds to intracellular PDZ-domain proteins and functions as a homophilic cell adhesion molecule at ... More

Lipid spirals in Bacillus subtilis and their role in cell division.

Authors:Barák I, Muchová K, Wilkinson AJ, O'Toole PJ, Pavlendová N,

Journal:Mol Microbiol

PubMed ID:18430139

The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along the long axis of cells ... More

View all FM™ 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide) citations