Glutamate receptor dynamics organizing synapse formation in vivo.
AuthorsRasse TM, Fouquet W, Schmid A, Kittel RJ, Mertel S, Sigrist CB, Schmidt M, Guzman A, Merino C, Qin G, Quentin C, Madeo FF, Heckmann M, Sigrist SJ
JournalNat Neurosci
PubMed ID16136672
'Insight into how glutamatergic synapses form in vivo is important for understanding developmental and experience-triggered changes of excitatory circuits. Here, we imaged postsynaptic densities (PSDs) expressing a functional, GFP-tagged glutamate receptor subunit (GluR-IIA(GFP)) at neuromuscular junctions of Drosophila melanogaster larvae for several days in vivo. New PSDs, associated with functional ... More
Fluorescence ratio imaging microscopy shows decreased access of vancomycin to cell wall synthetic sites in vancomycin-resistant Staphylococcus aureus.
AuthorsPereira PM, Filipe SR, Tomasz A, Pinho MG,
JournalAntimicrob Agents Chemother
PubMed ID17646417
'A new method of fluorescence ratio imaging microscopy was used to compare the in vivo binding capacity and the access of a fluorescent derivative of vancomycin to the cell wall synthetic sites in isogenic pairs of vancomycin-susceptible and -resistant laboratory mutants and vancomycin-intermediate and -susceptible clinical isolates of Staphylococcus aureus. ... More
Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity.
AuthorsLemke EA, Klingauf J
JournalJ Neurosci
PubMed ID16306416
'How synaptic vesicles move within central nervous synapses to their docking sites at the plasma membrane is widely discussed in synaptic physiology. This question is especially difficult to investigate in the small hippocampal boutons, which themselves can slowly move during observation in primary cell culture. Here, we describe a single ... More
SynCAM, a synaptic adhesion molecule that drives synapse assembly.
AuthorsBiederer T, Sara Y, Mozhayeva M, Atasoy D, Liu X, Kavalali ET, Südhof TC
JournalScience
PubMed ID12202822
'Synapses, the junctions between nerve cells through which they communicate, are formed by the coordinated assembly and tight attachment of pre- and postsynaptic specializations. We now show that SynCAM is a brain-specific, immunoglobulin domain-containing protein that binds to intracellular PDZ-domain proteins and functions as a homophilic cell adhesion molecule at ... More
Lipid spirals in Bacillus subtilis and their role in cell division.
AuthorsBarák I, Muchová K, Wilkinson AJ, O'Toole PJ, Pavlendová N,
JournalMol Microbiol
PubMed ID18430139
The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along the long axis of cells ... More
FM dyes enter via a store-operated calcium channel and modify calcium signaling of cultured astrocytes.
AuthorsLi D, Hérault K, Oheim M, Ropert N,
JournalProc Natl Acad Sci U S A
PubMed ID20007370
The amphiphilic fluorescent styryl pyridinium dyes FM1-43 and FM4-64 are used to probe activity-dependent synaptic vesicle cycling in neurons. Cultured astrocytes can internalize FM1-43 and FM4-64 inside vesicles but their uptake is insensitive to the elevation of cytosolic calcium (Ca(2+)) concentration and the underlying mechanism remains unclear. Here we used ... More
Remodeling of synaptic actin induced by photoconductive stimulation.
Authors Colicos M A; Collins B E; Sailor M J; Goda Y;
JournalCell
PubMed ID11733060
Use-dependent synapse remodeling is thought to provide a cellular mechanism for encoding durable memories, yet whether activity triggers an actual structural change has remained controversial. We use photoconductive stimulation to demonstrate activity-dependent morphological synaptic plasticity by video imaging of GFP-actin at individual synapses. A single tetanus transiently moves presynaptic actin ... More
Synaptic vesicles in rat hippocampal boutons recycle to different pools in a use-dependent fashion.
AuthorsVanden Berghe P, Klingauf J
JournalJ Physiol
PubMed ID16439431
Efficient vesicle membrane recycling at presynaptic terminals is pivotal for preventing depletion and maintaining high firing rates in neuronal networks. We used a new approach, based on the combination of spectrally different optical probes, to investigate how stimulation determines the fate of synaptic vesicles after endocytosis. We found that in ... More
The proteasome controls presynaptic differentiation through modulation of an on-site pool of polyubiquitinated conjugates.
Authors
JournalJ Cell Biol
PubMed ID27022091
The characteristics of insoluble softwood substrates affect fungal morphology, secretome composition, and hydrolytic efficiency of enzymes produced by Trichoderma reesei.
Authors
JournalBiotechnol Biofuels
PubMed ID33902680
Intralipid Increases Nitric Oxide Release from Human Endothelial Cells During Oxidative Stress.
Authors
JournalJPEN J Parenter Enteral Nutr
PubMed ID32291784
Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems.
Authors
JournalAoB Plants
PubMed ID32793329
CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc.
Authors
JournalMol Cell
PubMed ID34270916
Cultivation of a vampire: 'Candidatus Absconditicoccus praedator'.