来自人血清的转铁蛋白,Alexa Fluor™ 633 偶联物
来自人血清的转铁蛋白,Alexa Fluor™ 633 偶联物
Invitrogen™

来自人血清的转铁蛋白,Alexa Fluor™ 633 偶联物

转铁蛋白是一种单体血清糖蛋白(∼80,000 道尔顿),其通过受体介导的内吞作用结合到脊椎动物细胞表面的特定受体上,并递送多达 2 个 Fe3+ 原子 — 标记的了解更多信息
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货号数量
T233625 mg
货号 T23362
价格(CNY)
8,795.00
5 mg
添加至购物车
数量:
5 mg
价格(CNY)
8,795.00
5 mg
添加至购物车
转铁蛋白是一种单体血清糖蛋白(∼80,000 道尔顿),其通过受体介导的内吞作用结合到脊椎动物细胞表面的特定受体上,并递送多达 2 个 Fe3+ 原子 — 标记的 LDL 复合物是研究该现象的有用工具。携带铁的转铁蛋白进入核内体后,酸性环境即可促使铁离子从转铁蛋白–受体复合物中解离。铁释放后,脱铁转铁蛋白被回收至细胞膜中,其从受体中释放出来以结合更多的铁。因此,荧光转铁蛋白偶联物可以与荧光 LDL 配合使用,以区分溶菌体定向途径和内涵体回收通路。

这些实验通常是通过向培养细胞中加入荧光标记的转铁蛋白并通过显微镜进行分析。我们提供生物素化的转铁蛋白偶联物及超过 10 种荧光形式。

转铁蛋白规格:

标记 (Ex/Em):Alexa Fluor™ 633 (632/650)

含量:15 mg 固体(含 5 mg 转铁蛋白偶联物)

标记的转铁蛋白的主要应用
标记的转铁蛋白的其中一些应用包括:
•使用 FRET 对转铁蛋白受体动力学进行成像
• 通过共聚焦激光扫描显微镜检查观察活细胞中的受体转运
• 研究内涵体酸化期间发生的事件
• 测量哺乳动物和寄生虫中的转铁蛋白受体结合亲和力

仅供研究使用。不得用于任何动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型Alexa Fluor 染料
激发/发射632/650
形式实心
蛋白质家族转铁蛋白
数量5 mg
运输条件室温
产品线Alexa Fluor
产品类型转铁蛋白
pH7.2
Unit Size5 mg
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (19)

引用和文献
Abstract
CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family.
Authors:Perez F, Pernet-Gallay K, Nizak C, Goodson HV, Kreis TE, Goud B
Journal:J Cell Biol
PubMed ID:11854307
'The microtubule cytoskeleton plays a fundamental role in cell organization and membrane traffic in higher eukaryotes. It is well established that molecular motors are involved in membrane-microtubule interactions, but it has also been proposed that nonmotor microtubule-binding (MTB) proteins known as CLIPs (cytoplasmic linker proteins) have basic roles in these ... More
Geldanamycin stimulates internalization of ErbB2 in a proteasome-dependent way.
Authors:Lerdrup M, Hommelgaard AM, Grandal M, van Deurs B
Journal:J Cell Sci
PubMed ID:16352662
'The potent oncoprotein and receptor tyrosine kinase ErbB2 is remarkable because it resists efficient downregulation. However, ErbB2 can be downregulated by the HSP-90 inhibitor geldanamycin, but the underlying cellular mechanisms are uncertain. Apparently, delivery of ErbB2 to lysosomes, cleavage of the ErbB2 kinase domain and proteasomal activity are all processes ... More
Evaluation of disulfide reduction during receptor-mediated endocytosis by using FRET imaging.
Authors:Yang J, Chen H, Vlahov IR, Cheng JX, Low PS
Journal:Proc Natl Acad Sci U S A
PubMed ID:16950881
'Despite functional evidence for disulfide bond-reducing activity in endosomal compartments, the mechanistic details pertaining to such process (e.g., kinetics and sites of disulfide reduction) remain largely controversial. To address these questions directly, we have synthesized a previously uncharacterized fluorescent folate conjugate, folate-(BODIPY FL)-SS-rhodamine (folate-FRET), that changes fluorescence from red to ... More
Self-assembled, aptamer-tethered DNA nanotrains for targeted transport of molecular drugs in cancer theranostics.
Authors:Zhu G, Zheng J, Song E, Donovan M, Zhang K, Liu C, Tan W,
Journal:Proc Natl Acad Sci U S A
PubMed ID:23630258
'Nanotechnology has allowed the construction of various nanostructures for applications, including biomedicine. However, a simple target-specific, economical, and biocompatible drug delivery platform with high maximum tolerated doses is still in demand. Here, we report aptamer-tethered DNA nanotrains (aptNTrs) as carriers for targeted drug transport in cancer therapy. Long aptNTrs were ... More
Pincher, a pinocytic chaperone for nerve growth factor/TrkA signaling endosomes.
Authors:Shao Y, Akmentin W, Toledo-Aral JJ, Rosenbaum J, Valdez G, Cabot JB, Hilbush BS, Halegoua S
Journal:J Cell Biol
PubMed ID:12011113
A central tenet of nerve growth factor (NGF) action that is poorly understood is its ability to mediate cytoplasmic signaling, through its receptor TrkA, that is initiated at the nerve terminal and conveyed to the soma. We identified an NGF-induced protein that we termed Pincher (pinocytic chaperone) that mediates endocytosis ... More