CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family.
AuthorsPerez F, Pernet-Gallay K, Nizak C, Goodson HV, Kreis TE, Goud B
JournalJ Cell Biol
PubMed ID11854307
'The microtubule cytoskeleton plays a fundamental role in cell organization and membrane traffic in higher eukaryotes. It is well established that molecular motors are involved in membrane-microtubule interactions, but it has also been proposed that nonmotor microtubule-binding (MTB) proteins known as CLIPs (cytoplasmic linker proteins) have basic roles in these ... More
Geldanamycin stimulates internalization of ErbB2 in a proteasome-dependent way.
AuthorsLerdrup M, Hommelgaard AM, Grandal M, van Deurs B
JournalJ Cell Sci
PubMed ID16352662
'The potent oncoprotein and receptor tyrosine kinase ErbB2 is remarkable because it resists efficient downregulation. However, ErbB2 can be downregulated by the HSP-90 inhibitor geldanamycin, but the underlying cellular mechanisms are uncertain. Apparently, delivery of ErbB2 to lysosomes, cleavage of the ErbB2 kinase domain and proteasomal activity are all processes ... More
Evaluation of disulfide reduction during receptor-mediated endocytosis by using FRET imaging.
AuthorsYang J, Chen H, Vlahov IR, Cheng JX, Low PS
JournalProc Natl Acad Sci U S A
PubMed ID16950881
'Despite functional evidence for disulfide bond-reducing activity in endosomal compartments, the mechanistic details pertaining to such process (e.g., kinetics and sites of disulfide reduction) remain largely controversial. To address these questions directly, we have synthesized a previously uncharacterized fluorescent folate conjugate, folate-(BODIPY FL)-SS-rhodamine (folate-FRET), that changes fluorescence from red to ... More
Self-assembled, aptamer-tethered DNA nanotrains for targeted transport of molecular drugs in cancer theranostics.
AuthorsZhu G, Zheng J, Song E, Donovan M, Zhang K, Liu C, Tan W,
JournalProc Natl Acad Sci U S A
PubMed ID23630258
'Nanotechnology has allowed the construction of various nanostructures for applications, including biomedicine. However, a simple target-specific, economical, and biocompatible drug delivery platform with high maximum tolerated doses is still in demand. Here, we report aptamer-tethered DNA nanotrains (aptNTrs) as carriers for targeted drug transport in cancer therapy. Long aptNTrs were ... More
Pincher, a pinocytic chaperone for nerve growth factor/TrkA signaling endosomes.
AuthorsShao Y, Akmentin W, Toledo-Aral JJ, Rosenbaum J, Valdez G, Cabot JB, Hilbush BS, Halegoua S
JournalJ Cell Biol
PubMed ID12011113
A central tenet of nerve growth factor (NGF) action that is poorly understood is its ability to mediate cytoplasmic signaling, through its receptor TrkA, that is initiated at the nerve terminal and conveyed to the soma. We identified an NGF-induced protein that we termed Pincher (pinocytic chaperone) that mediates endocytosis ... More
Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction.
AuthorsLidke DS, Nagy P, Heintzmann R, Arndt-Jovin DJ, Post JN, Grecco HE, Jares-Erijman EA, Jovin TM
JournalNat Biotechnol
PubMed ID14704683
The erbB/HER family of transmembrane receptor tyrosine kinases (RTKs) mediate cellular responses to epidermal growth factor (EGF) and related ligands. We have imaged the early stages of RTK-dependent signaling in living cells using: (i) stable expression of erbB1/2/3 fused with visible fluorescent proteins (VFPs), (ii) fluorescent quantum dots (QDs) bearing ... More
Cardiac steroids induce changes in recycling of the plasma membrane in human NT2 cells.
AuthorsRosen H, Glukhman V, Feldmann T, Fridman E, Lichtstein D
JournalMol Biol Cell
PubMed ID14718569
Cardiac steroids (CSs) are specific inhibitors of Na+, K(+)-ATPase activity. Although the presence of CS-like compounds in animal tissues has been established, their physiological role is not evident. In the present study, treatment of human NT2 cells with physiological concentrations (nanomolar) of CSs caused the accumulation of large vesicles adjacent ... More
The use of inhibitors to study endocytic pathways of gene carriers: optimization and pitfalls.
AuthorsVercauteren D, Vandenbroucke RE, Jones AT, Rejman J, Demeester J, De Smedt SC, Sanders NN, Braeckmans K,
JournalMol Ther
PubMed ID20010917
Nonviral gene complexes can enter mammalian cells through different endocytic pathways. For efficient optimization of the gene carrier it is important to profile its cellular uptake, because this largely determines its intracellular processing and subsequent transfection efficiency. Most of the current information on uptake of these gene-delivery vehicles is based ... More
Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells.
AuthorsSelf TJ, Oakley SM, Hill SJ
JournalBr J Pharmacol
PubMed ID16086035
The aim of the present study was to investigate the cellular pathway involved in histamine-stimulated internalization of the human H1-receptor in CHO-K1 cells expressing N-terminal myc-tagged H1-receptor (Myc-H1) or N-terminal myc-C-terminal green fluorescent protein (Myc-GFP H1) versions of the receptor. Studies of 3H-mepyramine binding and histamine-stimulated 3H-inositol phosphate accumulation in ... More
Cytosolic entry of bisphosphonate drugs requires acidification of vesicles after fluid-phase endocytosis.
Bisphosphonates such as alendronate and zoledronate are blockbuster drugs used to inhibit osteoclast-mediated bone resorption. Although the molecular mechanisms by which bisphosphonates affect osteoclasts are now evident, the exact route by which they are internalized by cells is not known. To clarify this, we synthesized a novel, fluorescently labeled analog ... More
H-Ras dynamically interacts with recycling endosomes in CHO-K1 cells: involvement of Rab5 and Rab11 in the trafficking of H-Ras to this pericentriolar endocytic compartment.
AuthorsGomez GA, Daniotti JL
JournalJ Biol Chem
PubMed ID16079139
H-, N-, and K-Ras are isoforms of Ras proteins, which undergo different lipid modifications at the C terminus. These post-translational events make possible the association of Ras proteins both with the inner plasma membrane and to the cytosolic surface of endoplasmic reticulum and Golgi complex, which is also required for ... More
Identification of a novel Bves function: regulation of vesicular transport.
Authors
JournalEMBO J
PubMed ID20057356
Cdc48/VCP and Endocytosis Regulate TDP-43 and FUS Toxicity and Turnover.
Authors
JournalMol Cell Biol
PubMed ID31767634
A new inhibitor of the β-arrestin/AP2 endocytic complex reveals interplay between GPCR internalization and signalling.
Authors
JournalNat Commun
PubMed ID28416805
A Canstatin-Derived Peptide Provides Insight into the Role of Capillary Morphogenesis Gene 2 in Angiogenic Regulation and Matrix Uptake.