Tubulin Tracker™ Green（Oregon Green™ 488 Taxol，双乙酸酯），用于活细胞成像

引用和文献 (30)

Invitrogen™

Tubulin Tracker™ Green（Oregon Green™ 488 Taxol，双乙酸酯），用于活细胞成像

Tubulin Tracker™ Green（Oregon Green™ 488 Taxol，双乙酸酯）可对活细胞中聚合的微管蛋白进行绿色荧光染色。Tubulin Tracker™了解更多信息

货号	数量
T34075	1 set

货号 T34075

价格（CNY)

7,636.00

1 set

添加至购物车

1 set

价格（CNY)

7,636.00

1 set

添加至购物车

Tubulin Tracker™ Green（Oregon Green™ 488 Taxol，双乙酸酯）可对活细胞中聚合的微管蛋白进行绿色荧光染色。Tubulin Tracker™ Green 是一种不带电荷、不发荧光的化合物，可轻松穿过活细胞质膜。染料进入细胞后，非特异性酯酶会切割亲脂性封闭基团，形成带电荷的绿色荧光产物。

仅供科研使用。不可用于诊断程序。

染料类型Oregon Green™ 488

产品线Oregon Green

数量1 set

运输条件室温

标签类型经典染料

产品类型Tubulin Tracker

亚细胞定位微管蛋白、细胞骨架, Tubulin

Unit Size1 set

内容与储存

在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (30)

引用和文献

Abstract

PKC-induced intracellular trafficking of Ca(V)2 precedes its rapid recruitment to the plasma membrane.

Authors:Zhang Y, Helm JS, Senatore A, Spafford JD, Kaczmarek LK, Jonas EA,

Journal:J Neurosci

PubMed ID:18322103

'Activation of protein kinase C (PKC) potentiates secretion in Aplysia peptidergic neurons, in part by inducing new sites for peptide release at growth cone terminals. The mechanisms by which ion channels are trafficked to such sites are, however, not well understood. We now show that PKC activation rapidly recruits new ... More

Modulation of hippocampal calcium signalling and plasticity by serine/threonine protein phosphatases.

Authors:Koss DJ, Hindley KP, Riedel G, Platt B

Journal:J Neurochem

PubMed ID:17442047

'Kinases and phosphatases act antagonistically to maintain physiological phosphorylation/dephosphorylation at numerous intracellular sites critical for neuronal signalling. In this study, it was found that inhibition of serine/threonine phosphatases by exposure of hippocampal slices to okadaic acid (OA) or cantharidin (CA; 100 nmol/L) for 2 h resulted in reduced basal synaptic ... More

Redundant mechanisms recruit actin into the contractile ring in silkworm spermatocytes.

Authors:Chen W, Foss M, Tseng KF, Zhang D,

Journal:PLoS Biol

PubMed ID:18767903

'Cytokinesis is powered by the contraction of actomyosin filaments within the newly assembled contractile ring. Microtubules are a spindle component that is essential for the induction of cytokinesis. This induction could use central spindle and/or astral microtubules to stimulate cortical contraction around the spindle equator (equatorial stimulation). Alternatively, or in ... More

2-(Naphthalene-1-yl)-6-pyrrolidinyl-4-quinazolinone inhibits skin cancer M21 cell proliferation through aberrant expression of microtubules and the cell cycle.

Authors:Wu YC, Hour MJ, Leung WC, Wu CY, Liu WZ, Chang YH, Lee HZ,

Journal:J Pharmacol Exp Ther

PubMed ID:21652781

'Microtubules are a proven target for anticancer drug development because they are critical for mitotic spindle formation and the separation of chromosomes at mitosis. 2-(Naphthalene-1-yl)-6-pyrrolidinyl-4-quinazolinone (HL66) induced cell death with the large cells and multiple micronuclei in M21 skin cancer cells. We demonstrated that HL66-induced cell death is caspase-independent and ... More

Metalloprotease meprin beta generates nontoxic N-terminal amyloid precursor protein fragments in vivo.

Authors:Jefferson T, Cauševic M, auf dem Keller U, Schilling O, Isbert S, Geyer R, Maier W, Tschickardt S, Jumpertz T, Weggen S, Bond JS, Overall CM, Pietrzik CU, Becker-Pauly C,

Journal:J Biol Chem

PubMed ID:21646356

'Identification of physiologically relevant substrates is still the most challenging part in protease research for understanding the biological activity of these enzymes. The zinc-dependent metalloprotease meprin ß is known to be expressed in many tissues with functions in health and disease. Here, we demonstrate unique interactions between meprin ß and ... More

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