PKC-induced intracellular trafficking of Ca(V)2 precedes its rapid recruitment to the plasma membrane.
AuthorsZhang Y, Helm JS, Senatore A, Spafford JD, Kaczmarek LK, Jonas EA,
JournalJ Neurosci
PubMed ID18322103
'Activation of protein kinase C (PKC) potentiates secretion in Aplysia peptidergic neurons, in part by inducing new sites for peptide release at growth cone terminals. The mechanisms by which ion channels are trafficked to such sites are, however, not well understood. We now show that PKC activation rapidly recruits new ... More
Modulation of hippocampal calcium signalling and plasticity by serine/threonine protein phosphatases.
AuthorsKoss DJ, Hindley KP, Riedel G, Platt B
JournalJ Neurochem
PubMed ID17442047
'Kinases and phosphatases act antagonistically to maintain physiological phosphorylation/dephosphorylation at numerous intracellular sites critical for neuronal signalling. In this study, it was found that inhibition of serine/threonine phosphatases by exposure of hippocampal slices to okadaic acid (OA) or cantharidin (CA; 100 nmol/L) for 2 h resulted in reduced basal synaptic ... More
Redundant mechanisms recruit actin into the contractile ring in silkworm spermatocytes.
AuthorsChen W, Foss M, Tseng KF, Zhang D,
JournalPLoS Biol
PubMed ID18767903
'Cytokinesis is powered by the contraction of actomyosin filaments within the newly assembled contractile ring. Microtubules are a spindle component that is essential for the induction of cytokinesis. This induction could use central spindle and/or astral microtubules to stimulate cortical contraction around the spindle equator (equatorial stimulation). Alternatively, or in ... More
2-(Naphthalene-1-yl)-6-pyrrolidinyl-4-quinazolinone inhibits skin cancer M21 cell proliferation through aberrant expression of microtubules and the cell cycle.
AuthorsWu YC, Hour MJ, Leung WC, Wu CY, Liu WZ, Chang YH, Lee HZ,
JournalJ Pharmacol Exp Ther
PubMed ID21652781
'Microtubules are a proven target for anticancer drug development because they are critical for mitotic spindle formation and the separation of chromosomes at mitosis. 2-(Naphthalene-1-yl)-6-pyrrolidinyl-4-quinazolinone (HL66) induced cell death with the large cells and multiple micronuclei in M21 skin cancer cells. We demonstrated that HL66-induced cell death is caspase-independent and ... More
Metalloprotease meprin beta generates nontoxic N-terminal amyloid precursor protein fragments in vivo.
AuthorsJefferson T, Cauševic M, auf dem Keller U, Schilling O, Isbert S, Geyer R, Maier W, Tschickardt S, Jumpertz T, Weggen S, Bond JS, Overall CM, Pietrzik CU, Becker-Pauly C,
JournalJ Biol Chem
PubMed ID21646356
'Identification of physiologically relevant substrates is still the most challenging part in protease research for understanding the biological activity of these enzymes. The zinc-dependent metalloprotease meprin ß is known to be expressed in many tissues with functions in health and disease. Here, we demonstrate unique interactions between meprin ß and ... More
An incremental approach to automated protein localisation.
AuthorsTscherepanow M, Jensen N, Kummert F,
JournalBMC Bioinformatics
PubMed ID18937856
'BACKGROUND: The subcellular localisation of proteins in intact living cells is an important means for gaining information about protein functions. Even dynamic processes can be captured, which can barely be predicted based on amino acid sequences. Besides increasing our knowledge about intracellular processes, this information facilitates the development of innovative ... More
Protein kinase C delta-mediated cytoskeleton remodeling is involved in aloe-emodin-induced photokilling of human lung cancer cells.
AuthorsChang WT, You BJ, Yang WH, Wu CY, Bau DT, Lee HZ,
JournalAnticancer Res
PubMed ID22993309
'Photodynamic therapy is becoming a widely accepted form of cancer treatment using a photosensitizing agent and light. Our previous study has demonstrated that photoactivated aloe-emodin induced anoikis and changes in cell morphology, which were in part mediated through its effect on cytoskeleton in lung carcinoma H460 cells. However, the molecular ... More
Quantitative analysis of the role of receptor recycling in T cell polarization.
AuthorsArkhipov SN, Maly IV
JournalBiophys J
PubMed ID16963506
'Activation of T cells of the immune system involves recognition of the antigen by the T cell receptor and subsequent internalization and recycling of this receptor. We present a numerical model for this process that accounts for the polarity of the intracellular traffic determined by the polarization of the microtubule-organizing ... More
Microtubule-assisted mechanism for functional metabolic macromolecular complex formation.
AuthorsAn S, Deng Y, Tomsho JW, Kyoung M, Benkovic SJ,
JournalProc Natl Acad Sci U S A
PubMed ID20615962
Evidence has been presented for a metabolic multienzyme complex, the purinosome, that participates in de novo purine biosynthesis to form clusters in the cytoplasm of living cells under purine-depleted conditions. Here we identified, using fluorescent live cell imaging, that a microtubule network appears to physically control the spatial distribution of ... More
Cathelicidin suppresses colon cancer development by inhibition of cancer associated fibroblasts.
AuthorsCheng M, Ho S, Yoo JH, Tran DH, Bakirtzi K, Su B, Tran DH, Kubota Y, Ichikawa R, Koon HW,
Journal
PubMed ID25565877
Cathelicidin (LL-37 in humans and mCRAMP in mice) represents a family of endogenous antimicrobial and anti-inflammatory peptides. Cancer-associated fibroblasts can promote the proliferation of colon cancer cells and growth of colon cancer tumors. We examined the role of cathelicidin in the development of colon cancer, using subcutaneous human HT-29 colon-cancer-cell-derived ... More
Origin, composition, organization and function of the inner membrane complex of Plasmodium falciparum gametocytes.
The most virulent of the human malaria parasites, Plasmodium falciparum, undergoes a remarkable morphological transformation as it prepares itself for sexual reproduction and transmission via mosquitoes. Indeed P. falciparum is named for the unique falciform or crescent shape of the mature sexual stages. Once the metamorphosis is completed, the mature ... More
Reciprocal polarization of T and B cells at the immunological synapse.
AuthorsDuchez S, Rodrigues M, Bertrand F, Valitutti S,
JournalJ Immunol
PubMed ID21930964
Cognate interactions between T and B lymphocytes lead to the formation of the immunological synapse (IS) where bidirectional activation signals are exchanged. Although the molecular architecture and the function of the IS have been studied extensively on the T cell side, little is known about events occurring during synapse formation ... More
Intermediate filament-deficient cells are mechanically softer at large deformation: a multi-scale simulation study.
AuthorsBertaud J, Qin Z, Buehler MJ,
JournalActa Biomater
PubMed ID20102752
The cell's cytoskeleton, providing cells with structure and shape, consists of different structural proteins, including microtubules, actin microfilaments and intermediate filaments. It has been suggested that intermediate filaments play a crucial role in providing mechanical stability to cells. By utilizing a simple coarse-grained computational model of the intermediate filament network ... More
Microtubule assembly by the Apc protein is regulated by importin-beta--RanGTP.
AuthorsDikovskaya D, Li Z, Newton IP, Davidson I, Hutchins JR, Kalab P, Clarke PR, Näthke IS,
JournalJ Cell Sci
PubMed ID20144988
Mutations in the tumour suppressor Adenomatous polyposis coli (Apc) initiate most sporadic colorectal cancers. Apc is implicated in regulating microtubule (MT) dynamics in interphase and mitosis. However, little is known about the underlying mechanism or regulation of this Apc function. We identified importin-beta as a binding partner of Apc that ... More
Redistribution of actin during assembly and reassembly of the contractile ring in grasshopper spermatocytes.
AuthorsAlsop GB, Chen W, Foss M, Tseng KF, Zhang D,
JournalPLoS One
PubMed ID19287500
Cytokinesis in animal cells requires the assembly of an actomyosin contractile ring to cleave the cell. The ring is highly dynamic; it assembles and disassembles during each cell cleavage, resulting in the recurrent redistribution of actin. To investigate this process in grasshopper spermatocytes, we mechanically manipulated the spindle to induce ... More
Interaction of microtubules and actin during the post-fusion phase of exocytosis.
AuthorsMüller MT, Schempp R, Lutz A, Felder T, Felder E, Miklavc P
JournalSci Rep
PubMed ID31427591
'Exocytosis is the intracellular trafficking step where a secretory vesicle fuses with the plasma membrane to release vesicle content. Actin and microtubules both play a role in exocytosis; however, their interplay is not understood. Here we study the interaction of actin and microtubules during exocytosis in lung alveolar type II ... More
Cathelicidin Suppresses Colon Cancer Metastasis via a P2RX7-Dependent Mechanism.
AuthorsWang J, Cheng M, Law IKM, Ortiz C, Sun M, Koon HW,
JournalMol Ther Oncolytics
PubMed ID30847383
'The antimicrobial peptide cathelicidin inhibits development of colitis-associated colon cancer. However, the role of cathelicidin in colon cancer metastasis remains unknown. We hypothesized that cathelicidin is effective in inhibiting colon cancer metastasis. Human colon cancer HT-29 cells were injected intravenously into nude mice. Control HA-tagged adeno-associated virus (HA-AAV) or cathelicidin-overexpressing ... More
Presynaptic dystrophic neurites surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aß generation in Alzheimer's disease.
'Alzheimer''s disease (AD) is characterized by amyloid plaques composed of the ß-amyloid (Aß) peptide surrounded by swollen presynaptic dystrophic neurites consisting of dysfunctional axons and terminals that accumulate the ß-site amyloid precursor protein (APP) cleaving enzyme (BACE1) required for Aß generation. The cellular and molecular mechanisms that govern presynaptic dystrophic ... More
Elucidating target specificity of the taccalonolide covalent microtubule stabilizers employing a combinatorial chemical approach.
AuthorsDu L, Yee SS, Ramachandran K, Risinger AL
JournalNat Commun
PubMed ID32005831
'The taccalonolide microtubule stabilizers covalently bind ß-tubulin and overcome clinically relevant taxane resistance mechanisms. Evaluations of the target specificity and detailed drug-target interactions of taccalonolides, however, have been limited in part by their irreversible target engagement. In this study, we report the synthesis of fluorogenic taccalonolide probes that maintain the ... More
Structural responses of cells to intracellular magnetic force induced by superparamagnetic iron oxide nanoparticles.
Authors
JournalPhys Chem Chem Phys
PubMed ID24336693
Fenbendazole Controls In Vitro Growth, Virulence Potential, and Animal Infection in the Cryptococcus Model.
Authors
JournalAntimicrob Agents Chemother
PubMed ID32253211
Controllable microfluidic synthesis of multiphase drug-carrying lipospheres for site-targeted therapy.
Authors
JournalBiotechnol Prog
PubMed ID19455647
Cereblon Regulates the Proteotoxicity of Tau by Tuning the Chaperone Activity of DNAJA1.
Authors
JournalJ Neurosci
PubMed ID33972400
Characterization of glioblastoma in an orthotopic mouse model with magnetic resonance elastography.
Authors
JournalNMR Biomed
PubMed ID29193449
Anticancer drug released from near IR-activated prodrug overcomes spatiotemporal limits of singlet oxygen.
Authors
JournalBioorg Med Chem
PubMed ID26928287
Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens.
Authors
JournalACS Omega
PubMed ID33458495
Arrest of nuclear division in Plasmodium through blockage of erythrocyte surface exposed ribosomal protein P2.
Authors
JournalPLoS Pathog
PubMed ID22912579
Tunneling nanotube (TNT)-mediated neuron-to neuron transfer of pathological Tau protein assemblies.
AuthorsTardivel M, Bégard S, Bousset L, Dujardin S, Coens A, Melki R, Buée L, Colin M,
JournalActa Neuropathol Commun
PubMed ID27809932
A given cell makes exchanges with its neighbors through a variety of means ranging from diffusible factors to vesicles. Cells use also tunneling nanotubes (TNTs), filamentous-actin-containing membranous structures that bridge and connect cells. First described in immune cells, TNTs facilitate HIV-1 transfer and are found in various cell types, including ... More
Potential mechanisms of cellular injury following exposure to a physiologically relevant species of inorganic mercury.
AuthorsOrr SE, Barnes MC, Joshee L, Uchakina O, McKallip RJ, Bridges CC
JournalToxicol Lett
PubMed ID30630035
Mercury is a toxic metal that is found ubiquitously in the environment. Humans are exposed to different forms of mercury via ingestion, inhalation, and/or dermal absorption. Following exposure, mercuric ions may gain access to target cells and subsequently lead to cellular intoxication. The mechanisms by which mercury accumulation leads to ... More
Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction.
AuthorsMarkwirth A, Lachetta M, Mönkemöller V, Heintzmann R, Hübner W, Huser T, Müller M
JournalNat Commun
PubMed ID31541134
Super-resolved structured illumination microscopy (SR-SIM) is among the fastest fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-build instruments are able to deliver two-fold resolution enhancement with high acquisition speed. SR-SIM is usually a two-step process, with raw-data acquisition and subsequent, time-consuming post-processing for image reconstruction. In ... More