四甲基罗丹明,甲酯,高氯酸盐 (TMRM)
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
四甲基罗丹明,甲酯,高氯酸盐 (TMRM)
Invitrogen™

四甲基罗丹明,甲酯,高氯酸盐 (TMRM)

四甲基罗丹明甲酯 (TMRM) 是一种细胞通透性、阳离子型橙色荧光染料,可轻松吸附活性线粒体了解更多信息
Have Questions?
货号数量
T66825 mg
货号 T668
价格(CNY)
2,524.00
飞享价
Ends: 31-Dec-2025
3,421.00
共减 897.00 (26%)
25 mg
添加至购物车
数量:
25 mg
价格(CNY)
2,524.00
飞享价
Ends: 31-Dec-2025
3,421.00
共减 897.00 (26%)
25 mg
添加至购物车
四甲基罗丹明甲酯 (TMRM) 是一种细胞通透性、阳离子型橙色荧光染料,可轻松吸附活性线粒体。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
数量25 mg
运输条件室温
子细胞定位线粒体
颜色橙红色
适用于(设备)荧光显微镜
产品类型TMRM
Unit Size25 mg
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

当我使用膜电位指示剂时,看到神经元周围出现了较高的背景,如何降低背景干扰?

如果使用我们的FluoVolt 膜电势试剂盒(货号F10488),该试剂盒包含一种背景抑制剂,可改善这一问题。对于其他指标剂,可以考虑使用BackDrop 背景抑制剂(货号R37603、B10511和B10512)。

快反应膜电位探针和慢反应膜电位探针有什么区别?

在周围电场的作用下结构变化的分子可用作检测瞬时(毫秒级)电位变化的快反应探针。慢反应染料则会进入去极化细胞,结合蛋白或膜。增强去极化会造成额外的染料流入,增强荧光强度;过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像,或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

你们提供哪些类型的膜电位指示剂?我该如何根据自己的试验选择?

膜电位指示剂选择指南请见此处(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html)。

当我测试线粒体膜电位时,未处理的细胞也会发出荧光,而且我在试验样品中没有看到显著差异。

无论您使用哪种染料,四甲基罗丹明甲酯(TMRM)还是MitoTracker Red FM,未处理的细胞都会发出荧光。只要细胞线粒体膜电位降低就会导致荧光信号降低。最重要的是变化的程度。JC-1染料不仅强度改变,还有激发和发射比例光谱的改变。设置未处理的对照和用线粒体膜电位去稳定剂(如CCCP或FCCP)处理的阳性对照是非常重要的。这些染料仅用于活细胞,在固定处理的细胞中无法保留相同程度的信号。

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (186)

引用和文献
Abstract
Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones.
Authors:Coatesworth W, Bolsover S
Journal:Cell Calcium
PubMed ID:16338004
'A brief depolarisation of chick sensory neurones evokes a calcium increase in mitochondria that peaks 1-2s after the depolarisation event and then decays over tens of seconds. Peripheral mitochondria take up more calcium than do central ones, even when the cytosolic calcium increase is spatially homogeneous. The calcium influx into ... More
Tissue transglutaminase is a multifunctional BH3-only protein.
Authors:Rodolfo C, Mormone E, Matarrese P, Ciccosanti F, Farrace MG, Garofano E, Piredda L, Fimia GM, Malorni W, Piacentini M
Journal:J Biol Chem
PubMed ID:15485857
'Tissue transglutaminase (TG2) protein accumulates to high levels in cells during early stages of apoptosis both in vivo and in vitro. The analysis of the TG2 primary sequence showed the presence of an eight amino acid domain, sharing 70% identity with the Bcl-2 family BH3 domain. Cell-permeable peptides, mimicking the ... More
Methods for assessing autophagy and autophagic cell death.
Authors:Tasdemir E, Galluzzi L, Maiuri MC, Criollo A, Vitale I, Hangen E, Modjtahedi N, Kroemer G,
Journal:Methods Mol Biol
PubMed ID:18425442
'Autophagic (or type 2) cell death is characterized by the massive accumulation of autophagic vacuoles (autophagosomes) in the cytoplasm of cells that lack signs of apoptosis (type 1 cell death). Here we detail and critically assess a series of methods to promote and inhibit autophagy via pharmacological and genetic manipulations. ... More
Trafficking of ganglioside GD3 to mitochondria by tumor necrosis factor-alpha.
Authors:García-Ruiz C, Colell A, Morales A, Calvo M, Enrich C, Fernández-Checa JC
Journal:J Biol Chem
PubMed ID:12118012
'The interaction of mitochondria with proapoptotic proteins activates apoptosis pathways. Previous findings have identified ganglioside GD3 (GD3) as an emerging apoptotic lipid intermediate that targets mitochondria in response to death signals. Using immunoelectron and laser scanning confocal microscopy, we characterize the trafficking of GD3 to mitochondria in response to tumor ... More
Unreliability of the cytochrome c-enhanced green fluorescent fusion protein as a marker of cytochrome c release in cells that overexpress Bcl-2.
Authors:Unkila M, McColl KS, Thomenius MJ, Heiskanen K, Distelhorst CW
Journal:J Biol Chem
PubMed ID:11489892
'A cytochrome c-enhanced green fluorescent protein chimera (cyt-c.EGFP) was used to monitor the release of cytochrome c from mitochondria in Bcl-2-negative and Bcl-2-positive MDA-MB-468 breast cancer cells. A comparison was made with the intracellular distribution of endogenous cytochrome c based on Western blotting of cell fractions and immunocytochemistry. The release ... More