A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution.
AuthorsBegum J, Day W, Henderson C, Purewal S, Cerviera J, Summers H, Rees P, Davies D, Filby A,
Journal
PubMed ID24166880
Labeling nonquiescent cells with carboxyfluorescein succinimidyl ester (CFSE)-like dyes gives rise to a population width exceeding the threshold for resolving division peaks by flow cytometry. Width is a function of biological heterogeneity plus extrinsic and intrinsic error sources associated with the measurement process. Optimal cytometer performance minimizes extrinsic error, but ... More