含有 Annexin V Alexa Fluor 488 和 PI 的死细胞凋亡试剂盒 含有 Annexin V Alexa Fluor 488 和碘化丙啶 (PI) 的死细胞凋亡试剂盒 (V13241, V13245) 用于进行流式细胞分析,通过检测磷脂酰丝氨酸 (PS) 表达和膜通透性来测量早期细胞凋亡。当细胞用 Annexin V 和碘化丙啶染色时,表达 PS 的凋亡细胞显示绿色荧光,可在 FITC 通道中检测到,并显示低红色荧光。死亡或坏死细胞显示明亮的红色荧光,无绿色荧光,而活细胞显示无绿色或红色荧光。
含有 Annexin V PE 和 SYTOX Green 的死细胞凋亡试剂盒 含有 Annexin V PE 和 SYTOX Green 的死细胞凋亡试剂盒可在流式细胞分析期间使用偶联橙色荧光藻胆蛋白 R-PE 的重组膜联蛋白 V 检测凋亡细胞的 PS 外化,死细胞则用 SYTOX Green 核酸染色剂进行检测。经过两种探针处理后,凋亡细胞显示橙色荧光,死细胞显示绿色荧光,活细胞几乎不显示荧光。在使用 488 nm 激光流式细胞仪的 530/30 nm 和 585/42 nm 带通滤光片中可轻松区分这些细胞群。
含有 Annexin V APC 和 SYTOX Green 的死细胞凋亡试剂盒 含有 Annexin V APC 和 SYTOX Green 的死细胞凋亡试剂盒可在流式细胞分析期间使用偶联红色激光激发别藻蓝蛋白的重组膜联蛋白 V 检测凋亡细胞的 PS 外化,死细胞则用 SYTOX Green 核酸染色剂进行检测。通过与外化 PS 结合的膜联蛋白 V 检测凋亡细胞。活细胞几乎不显示荧光,凋亡细胞显示红色荧光和极少的绿色荧光,晚期凋亡细胞显示较高水平的红色和橙色荧光。这些细胞群可以很容易地通过含有 488 nm 和 633 nm 激发源(氩离子激光器和 HeNe 激光器)的流式细胞仪加以区分。
含 Annexin V FITC 和 PI 的死细胞凋亡试剂盒 含 Annexin V FITC 和 PI 的死细胞凋亡试剂盒可使用偶联绿色荧光 FITC 染料的重组膜联蛋白 V 检测凋亡细胞的 PS 外化,死细胞则用 PI 进行检测。经 PI 染色的坏死细胞显示红色荧光。两种探针处理后,凋亡细胞显示绿色荧光,死细胞显示红色和绿色荧光,活细胞显示少量或无荧光。
I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?
It has been done, but we dont recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.
Journal:Journal of immunology (Baltimore, Md. : 1950)
PubMed ID:17548610
Chitin is a size-dependent regulator of macrophage TNF and IL-10 production.
Authors:Da Silva CA, Chalouni C, Williams A, Hartl D, Lee CG, Elias JA,
Journal:J Immunol
PubMed ID:19265136
'Chitin is a ubiquitous polysaccharide in fungi, insects, and parasites. We hypothesized that chitin is a size-dependent regulator of innate immunity. To test this hypothesis, we characterized the effects of chitins of different sizes on murine bronchoalveolar or peritoneal macrophages. In these studies, large chitin fragments were inert, while both ... More
The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis.
Authors:Raasi S, Schmidtke G, Groettrup M
Journal:J Biol Chem
PubMed ID:11445583
'FAT10 is a ubiquitin-like protein that is encoded in the major histocompatibility complex class I locus and is synergistically inducible with interferon-gamma and tumor necrosis factor alpha. The molecule consists of two ubiquitin-like domains in tandem arrangement and bears a conserved diglycine motif at its carboxyl terminus commonly used in ... More
Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
Authors:Soldani C, Croce AC, Bottone MG, Fraschini A, Biggiogera M, Bottiroli G, Pellicciari C,
Journal:Histochem Cell Biol
PubMed ID:17849139
'Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored ... More
Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion protein.
Authors:Jain S, McGinnes LW, Morrison TG
Journal:J Virol
PubMed ID:17151113
'Newcastle disease virus (NDV), an avian paramyxovirus, initiates infection with attachment of the viral hemagglutinin-neuraminidase (HN) protein to sialic acid-containing receptors, followed by fusion of viral and cell membranes, which is mediated by the fusion (F) protein. Like all class 1 viral fusion proteins, the paramyxovirus F protein is thought ... More