JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID17548610
Chitin is a size-dependent regulator of macrophage TNF and IL-10 production.
AuthorsDa Silva CA, Chalouni C, Williams A, Hartl D, Lee CG, Elias JA,
JournalJ Immunol
PubMed ID19265136
'Chitin is a ubiquitous polysaccharide in fungi, insects, and parasites. We hypothesized that chitin is a size-dependent regulator of innate immunity. To test this hypothesis, we characterized the effects of chitins of different sizes on murine bronchoalveolar or peritoneal macrophages. In these studies, large chitin fragments were inert, while both ... More
The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis.
AuthorsRaasi S, Schmidtke G, Groettrup M
JournalJ Biol Chem
PubMed ID11445583
'FAT10 is a ubiquitin-like protein that is encoded in the major histocompatibility complex class I locus and is synergistically inducible with interferon-gamma and tumor necrosis factor alpha. The molecule consists of two ubiquitin-like domains in tandem arrangement and bears a conserved diglycine motif at its carboxyl terminus commonly used in ... More
Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
AuthorsSoldani C, Croce AC, Bottone MG, Fraschini A, Biggiogera M, Bottiroli G, Pellicciari C,
JournalHistochem Cell Biol
PubMed ID17849139
'Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored ... More
Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion protein.
AuthorsJain S, McGinnes LW, Morrison TG
JournalJ Virol
PubMed ID17151113
'Newcastle disease virus (NDV), an avian paramyxovirus, initiates infection with attachment of the viral hemagglutinin-neuraminidase (HN) protein to sialic acid-containing receptors, followed by fusion of viral and cell membranes, which is mediated by the fusion (F) protein. Like all class 1 viral fusion proteins, the paramyxovirus F protein is thought ... More
Cell type specific applicability of 5-ethynyl-2'-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry.
AuthorsDiermeier-Daucher S, Clarke ST, Hill D, Vollmann-Zwerenz A, Bradford JA, Brockhoff G,
JournalCytometry A
PubMed ID19235202
'Using the nucleoside analogue EdU (5-ethynyl-2''-deoxyuridine) for thymidine substitution instead of BrdU (5-bromo-2''-deoxyuridine) in cell proliferation assays has recently been proposed. However, the effect of EdU on cell viability, DNA synthesis, and cell cycle progression and consequently its usability for dynamic cell proliferation analysis in vitro has not been explored. ... More
CpG oligodeoxynucleotide protection in polymicrobial sepsis is dependent on interleukin-17.
'CpG oligodeoxynucleotides (ODNs) may prevent mortality from infection. We have identified a therapeutic benefit in treating sepsis with phosphorothioate ODN sequences containing the CpG motif. Sepsis was induced in rats by cecal ligation and puncture (CLP), and treatment with CpG ODNs reduced sepsis mortality from 80% to 15% during a ... More
Bcl-2 protects against FCCP-induced apoptosis and mitochondrial membrane potential depolarization in PC12 cells.
AuthorsDispersyn G, Nuydens R, Connors R, Borgers M, Geerts H
JournalBiochim Biophys Acta
PubMed ID10434055
'This report addresses the relation between Bcl-2 and mitochondrial membrane potential (DeltaPsi(m)) in apoptotic cell death. Rat pheochromocytoma (PC12) cells are differentiated into neuron-like cells with nerve growth factor (NGF). It is known that Bcl-2 can attenuate apoptosis induced by deprivation of neurotrophic factor. The protective effect of Bcl-2 has ... More
Aeromonas hydrophila cytotoxic enterotoxin activates mitogen-activated protein kinases and induces apoptosis in murine macrophages and human intestinal epithelial cells.
AuthorsGalindo CL, Fadl AA, Sha J, Gutierrez C, Popov VL, Boldogh I, Aggarwal BB, Chopra AK
JournalJ Biol Chem
PubMed ID15215244
'A cytotoxic enterotoxin (Act) of Aeromonas hydrophila possesses several biological activities, induces an inflammatory response in the host, and causes apoptosis of murine macrophages. In this study, we utilized five target cell types (a murine macrophage cell line (RAW 264.7), bone marrow-derived transformed macrophages, murine peritoneal macrophages, and two human ... More
Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.
AuthorsGrandl M, Schmitz G,
JournalCytometry A
PubMed ID20014301
'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More
Expression, purification and use of recombinant annexin V for the detection of apoptotic cells.
AuthorsLogue SE, Elgendy M, Martin SJ,
JournalNat Protoc
PubMed ID19730422
'Apoptosis is a mode of programmed cell death that is widely used to eliminate cells during development, tissue homeostasis, infection or in response to injury. Alterations to the plasma membranes of apoptotic cells trigger recognition and engulfment of such cells by phagocytes. Measurement of plasma membrane phosphatidylserine externalization, using fluorescently ... More
Phosphoaspirin (MDC-43), a novel benzyl ester of aspirin, inhibits the growth of human cancer cell lines more potently than aspirin: a redox-dependent effect.
'Aspirin is chemopreventive against colon and probably other cancers, but this effect is relatively weak and its chronic administration to humans is associated with significant side effects. Because of these limitations, extensive effort has been exerted to improve the pharmacological properties of aspirin. We have determined the anticancer activity and ... More
Differential requirement for caspase 9 in apoptotic pathways in vivo.
AuthorsHakem R, Hakem A, Duncan GS, Henderson JT, Woo M, Soengas MS, Elia A, de la Pompa JL, Kagi D, Khoo W, Potter J, Yoshida R, Kaufman SA, Lowe SW, Penninger JM, Mak TW
JournalCell
PubMed ID9708736
'Mutation of Caspase 9 (Casp9) results in embryonic lethality and defective brain development associated with decreased apoptosis. Casp9-/- embryonic stem cells and embryonic fibroblasts are resistant to several apoptotic stimuli, including UV and gamma irradiation. Casp9-/- thymocytes are also resistant to dexamethasone- and gamma irradiation-induced apoptosis, but are surprisingly sensitive ... More
Pseudomonas aeruginosa-induced apoptosis is defective in respiratory epithelial cells expressing mutant cystic fibrosis transmembrane conductance regulator.
AuthorsCannon CL, Kowalski MP, Stopak KS, Pier GB
JournalAm J Respir Cell Mol Biol
PubMed ID12878584
'Chronic lung infection with Pseudomonas aeruginosa constitutes the most severe manifestation of cystic fibrosis, a scenario that results from defects in early clearance of the microbe. Early clearance involves epithelial cell ingestion of bacteria, rapid activation of nuclear factor-kappa B and cellular desquamation within minutes of P. aeruginosa infection, processes ... More
Myeloperoxidase is a key regulator of oxidative stress mediated apoptosis in myeloid leukemic cells.
AuthorsNakazato T, Sagawa M, Yamato K, Xian M, Yamamoto T, Suematsu M, Ikeda Y, Kizaki M,
JournalClin Cancer Res
PubMed ID17875773
'PURPOSE: We reported previously that reactive oxygen species (ROS) are key mediators of apoptosis induced by a polyphenol, (-)-epigallocatechin-3-gallate (EGCG), in myeloid leukemic cells. This study aimed to further examine the mechanism of ROS-mediated apoptosis induced by EGCG and its relationship to the heme enzyme myeloperoxidase (MPO). EXPERIMENTAL DESIGN: We ... More
A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells.
AuthorsDing W, Cao Y, Xing F, Tao M, Fu H, Luo H, Yang X
Journal
PubMed ID31148871
'Vascular endothelial growth factor (VEGF) is significantly elevated in psoriatic patients and is associated with the severity of the psoriasis. Due to the effect of inhibiting production of VEGF, acitretin can effectively treat psoriasis. Semaphorin 3A (Sema3A) restrain tumor growth and angiogenesis by partially reversing VEGF effects on tumor. However, ... More
Flow cytometry-based apoptosis detection.
AuthorsWlodkowic D, Skommer J, Darzynkiewicz Z,
JournalMethods Mol Biol
PubMed ID19609746
'An apoptosing cell demonstrates multitude of characteristic morphological and biochemical features, which vary depending on the stimuli and the cell type. The gross majority of classical apoptotic hallmarks can be rapidly examined by flow and image cytometry. Cytometry thus became a technology of choice in diverse studies of cellular demise. ... More
A cell nanoinjector based on carbon nanotubes.
AuthorsChen X, Kis A, Zettl A, Bertozzi CR,
JournalProc Natl Acad Sci U S A
PubMed ID17485677
Technologies for introducing molecules into living cells are vital for probing the physical properties and biochemical interactions that govern the cell's behavior. Here, we report the development of a nanoscale cell injection system (termed the nanoinjector) that uses carbon nanotubes to deliver cargo into cells. A single multiwalled carbon nanotube ... More
Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane.
AuthorsPakhomov AG, Bowman AM, Ibey BL, Andre FM, Pakhomova ON, Schoenbach KH,
JournalBiochem Biophys Res Commun
PubMed ID19450553
Cell permeabilization by electric pulses (EPs), or electroporation, has been well established as a tool to indiscriminately increase membrane flows of water solutes down the concentration and voltage gradients. However, we found that EPs of nanosecond duration (nsEPs) trigger formation of voltage-sensitive and inward-rectifying membrane pores. NsEP-treated cells remain mostly ... More
Isophilippinolide A arrests cell cycle progression and induces apoptosis for anticancer inhibitory agents in human melanoma cells.
AuthorsWang HM, Chen CY, Wu PF,
Journal
PubMed ID24359513
Three new butanolides, isophilippinolide A, philippinolide A, and philippinolide B, and an amide, cinnaretamine, were isolated from the roots of Cinnamomum philippinense to be identified by spectroscopic analysis. Four isolated compounds were screened to examine their radical-scavenging ability, metal-chelating power, and ferric-reducing antioxidant power assay (FRAP). Cinnaretamine showed powerful antioxidative ... More
Fluorescent biomembrane probe for ratiometric detection of apoptosis.
AuthorsShynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Freyssinet JM, Mely Y,
JournalJ Am Chem Soc
PubMed ID17256940
Herein, we developed the first ratiometric fluorescent probe for apoptosis detection. This probe incorporates selectively into the outer leaflet of the cell plasma membrane and senses the loss of the plasma membrane asymmetry occurring during the early steps of apoptosis. The high specificity to the plasma membranes was achieved by ... More
Screen for chemical modulators of autophagy reveals novel therapeutic inhibitors of mTORC1 signaling.
AuthorsBalgi AD, Fonseca BD, Donohue E, Tsang TC, Lajoie P, Proud CG, Nabi IR, Roberge M,
JournalPLoS One
PubMed ID19771169
BACKGROUND: Mammalian target of rapamycin complex 1 (mTORC1) is a protein kinase that relays nutrient availability signals to control numerous cellular functions including autophagy, a process of cellular self-eating activated by nutrient depletion. Addressing the therapeutic potential of modulating mTORC1 signaling and autophagy in human disease requires active chemicals with ... More
Sulfated glycosphingolipid as mediator of phagocytosis: SM4s enhances apoptotic cell clearance and modulates macrophage activity.
AuthorsPopovic ZV, Sandhoff R, Sijmonsma TP, Kaden S, Jennemann R, Kiss E, Tone E, Autschbach F, Platt N, Malle E, Gröne HJ,
JournalJ Immunol
PubMed ID17982067
Sulfoglycolipids are present on the surface of a variety of cells. The sulfatide SM4s is increased in lung, renal, and colon cancer and is associated with an adverse prognosis, possibly due to a low immunoreactivity of the tumor. As macrophages significantly contribute to the inflammatory infiltrate in malignancies, we postulated ... More
Reactive Oxygen Species-Activated Akt/ASK1/p38 Signaling Pathway in Nickel Compound-Induced Apoptosis in BEAS 2B Cells.
AuthorsPan J, Chang Q, Wang X, Son Y, Zhang Z, Chen G, Luo J, Bi Y, Chen F, Shi X,
JournalChem Res Toxicol
PubMed ID20112989
Nickel compounds are carcinogenic to humans, possibly through induction of reactive oxygen species (ROS) that damage macromolecules including DNA and proteins. The aim of the present study is to elucidate the role of the ROS-mediated Akt/apoptosis-regulating signal kinase (ASK) 1/p38 pathway in nickel-induced apoptosis. Exposure of human bronchial epithelial cells ... More
Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.
AuthorsKim YC, Kim BG, Lee JH,
JournalPLoS One
PubMed ID22623951
Thymosin ß(10) (Tß(10)) regulates actin dynamics as a cytoplasm G-actin sequestering protein. Previously, we have shown that Tß(10) diminishes tumor growth, angiogenesis, and proliferation by disrupting actin and by inhibiting Ras. However, little is known about its mechanism of action and biological function. In the present study, we establish a ... More
Comparison of apoptosis and mortality measurements in peripheral blood mononuclear cells (PBMCs) using multiple methods.
Death through apoptosis is the main process by which aged cells that have lost their function are eliminated. Apoptotic cells are usually detected microscopically by changes in their morphology. However, determination of early apoptotic events is important for in vitro (and ex vivo) studies. The main objective of the present ... More
Nitric oxide mediates T cell cytokine production and signal transduction in histidine decarboxylase knockout mice.
AuthorsKoncz A, Pasztoi M, Mazan M, Fazakas F, Buzas E, Falus A, Nagy G,
JournalJ Immunol
PubMed ID17982051
Histamine is a key regulator of the immune system. Several lines of evidence suggest the role of histamine in T cell activation and accelerated Th1 immune response is a hallmark of histidine decarboxylase knockout (HDC-KO) mice, with a complete lack of endogenously produced histamine. According to our previous work, T ... More
Automatic image analysis for quantification of apoptosis in animal cell culture by annexin-V affinity assay.
AuthorsPläsier B, Lloyd DR, Paul GC, Thomas CR, Al-Rubeai M
JournalJ Immunol Methods
PubMed ID10556693
Apoptosis is a form of cell death in which the dying cell plays an active part in its demise. At the morphological level, it is characterised by cell shrinkage rather than the swelling seen in necrotic cell death. In cell culture, apoptosis limits the yield of economically and medically important ... More
Monocytic cell necrosis is mediated by potassium depletion and caspase-like proteases.
AuthorsWarny M, Kelly CP
JournalAm J Physiol
PubMed ID10070000
Apoptosis is a physiological cell death that culminates in mitochondrial permeability transition and the activation of caspases, a family of cysteine proteases. Necrosis, in contrast, is a pathological cell death characterized by swelling of the cytoplasm and mitochondria and rapid plasma membrane disruption. Necrotic cell death has long been opposed ... More
The apoptosis-necrosis paradox. Apoptogenic proteases activated after mitochondrial permeability transition determine the mode of cell death.
AuthorsHirsch T, Marchetti P, Susin SA, Dallaporta B, Zamzami N, Marzo I, Geuskens M, Kroemer G
JournalOncogene
PubMed ID9380409
Mitochondrial alterations including permeability transition (PT) constitute critical events of the apoptotic cascade and are under the control of Bcl-2 related gene products. Here we show that induction of PT is sufficient to activate CPP32-like proteases with DEVDase activity and the associated cleavage of the nuclear DEVDase substrate poly(ADP-ribose) polymerase ... More
Expression of constitutively active phosphatidylinositol 3-kinase inhibits activation of caspase 3 and apoptosis of cardiac muscle cells.
AuthorsWu W, Lee WL, Wu YY, Chen D, Liu TJ, Jang A, Sharma PM, Wang PH
JournalJ Biol Chem
PubMed ID11007772
Apoptosis of cardiac muscle cells contributes to the development of cardiomyopathy. Recent studies showed that insulin-like growth factor I (IGF-I) inhibits apoptosis of cardiac muscle cells and improves myocardial function in experimental heart failure. This study was carried out to elucidate the role of phosphatidylinositol 3-kinase (PI 3-kinase) in the ... More
Mitotically heritable effects of BMAA on striatal neural stem cell proliferation and differentiation.
AuthorsPierozan P, Karlsson O
JournalCell Death Dis
PubMed ID31209203
The widespread environmental contaminant ß-methylamino-L-alanine (BMAA) is a developmental neurotoxicant that can induce long-term learning and memory deficits. Studies have shown high transplacental transfer of 3H-BMAA and a significant uptake in fetal brain. Therefore, more information on how BMAA may influence growth and differentiation of neural stem cells is required ... More
Mesenchymal stem cells rejuvenate cardiac muscle after ischemic injury.
AuthorsZhang B, Zhang J, Zhu D, Kong Y
JournalAging (Albany NY)
PubMed ID30613028
Previous studies have shown that transplantation of mesenchymal stem cells (MSCs) enhances myocardial regeneration after myocardial infarction (MI), primarily resulting from the production and release of trophic growth factors and cytokines by MSCs. However, effects of MSCs or a subtype of MSCs on the ageing of injured cardiac muscle cells ... More
WD-3 inhibits the proliferation of breast cancer cells by regulating the glycolytic pathway.
AuthorsZhu X, Zhao L, You J, Ni Y, Wei Z, Xue Q, Jin C
JournalBosn J Basic Med Sci
PubMed ID32020848
Number 3 Prescription (WD-3) is an herbal remedy used in traditional Chinese medicine that has been shown to improve the outcomes of patients with advanced colon and gastric cancers. This study aimed to investigate the effect of WD-3 on proliferation, glycolysis, and hexokinase 2 expression in breast cancer cells. Four ... More