Vybrant™ Apoptosis Assay Kits for apoptotic and necrotic cell staining, 200 assays - FAQs

查看更多产品信息 Vybrant™ Apoptosis Assay Kits for apoptotic and necrotic cell staining - FAQs (V13244, V13243, V23200)

3 个常见问题解答

什么是细胞连续凋亡的相对期间时限，我该用什么样的产品去检测不同的凋亡事件？

见本文(https://www.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/support/bioprobes/bioprobes-65.par.61751.file.dat/bioprobes-65-cellevent.pdf)的表1，在0-4小时的期间，用10 µM喜树碱诱导Jurkat细胞。值得注意的是，这些结果是通过使用单细胞类型和诱导体系研究得到的；对于其他实验体系结果可能不同。凋亡细胞的细胞膜通透性增加，使用YO-PRO-1染料结合碘化丙啶或SYTOX死细胞指示剂能将其与死细胞区分开。其他中期凋亡事件是细胞ROS产物增多（用CellROX reagents，H2DCFDA试剂检测），细胞的pH变化（BCECF, SNARF-1）和钙离子释放（Fluo-4, Fura-2, Indo-1）。任何条件下，没有单个参数能够定义凋亡，因此研究凋亡时最好采用多种参数的方法

What are the fluorescence excitation/emission maxima for the dyes in the Membrane Permeability/Dead Cell Apoptosis Kit with YO-PRO-1 and PI for Flow Cytometry aka Vybrant Apoptosis Assay Kit #4, YO-PRO-1/Propidium Iodide)?

YO-PRO-1: 491/509 nm, bound to DNA
Propidium iodide: 535/617 nm, bound to DNA

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What is the relative time-frame of apoptosis progression, and what products can I use to detect the different apoptotic events?

See Table 1 in this article (http://www.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/support/bioprobes/bioprobes-65.par.61751.file.dat/bioprobes-65-cellevent.pdf) where a Jurkat model system was induced with 10 µM camptothecin for time periods of 0 to 4 hours. It is important to note that these results were studied using a single cell type and induction system; results may differ for other experimental systems. Increased membrane permeability in apoptotic cells can be discriminated from dead cells using YO-PRO-1 dye in combination with propidium iodide or the SYTOX dead cell indicators. Other mid-apoptotic events are increased ROS production (detected with CellROX reagents, H2DCFDA), changes in cellular pH (BCECF, SNARF-1) and calcium release (Fluo-4, Fura-2, Indo-1). No single parameter defines apoptosis under any condition, so it is best to employ a multi-parametric approach when studying apoptosis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.