Gap junction adhesion is necessary for radial migration in the neocortex.
AuthorsElias LA, Wang DD, Kriegstein AR,
JournalNature
PubMed ID17713529
'Radial glia, the neuronal stem cells of the embryonic cerebral cortex, reside deep within the developing brain and extend radial fibres to the pial surface, along which embryonic neurons migrate to reach the cortical plate. Here we show that the gap junction subunits connexin 26 (Cx26) and connexin 43 (Cx43) ... More
Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1.
AuthorsGazit R, Gruda R, Elboim M, Arnon TI, Katz G, Achdout H, Hanna J, Qimron U, Landau G, Greenbaum E, Zakay-Rones Z, Porgador A, Mandelboim O
JournalNat Immunol
PubMed ID16565719
The elimination of viruses and tumors by natural killer cells is mediated by specific natural killer cell receptors. To study the in vivo function of a principal activating natural killer cell receptor, NCR1 (NKp46 in humans), we replaced the gene encoding this receptor (Ncr1) with a green fluorescent protein reporter ... More
Adaptability, sensitivity, resolution and non-invasiveness are the attributes that have contributed to the longstanding use of light as an investigational tool and form the basis of optical imaging (OI). OI, which encompasses numerous techniques and methods, is rapid (<5 min), inexpensive, noninvasive, nontoxic (no radiation) and has molecular (single-cell) sensitivity, ... More
Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells.
AuthorsShalek AK, Robinson JT, Karp ES, Lee JS, Ahn DR, Yoon MH, Sutton A, Jorgolli M, Gertner RS, Gujral TS, Macbeath G, Yang EG, Park H,
JournalProc Natl Acad Sci U S A
PubMed ID20080678
A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies ... More
Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils.
In gradients of external chemo-attractant, mammalian neutrophilic leukocytes (neutrophils) and Dictyostelium discoideum amoebae adopt a polarized morphology and selectively accumulate lipid products of phosphatidylinositol-3-OH kinases (PI(3)Ks), including PtdIns(3,4,5)P(3), at their up-gradient edges; the internal PtdIns(3,4,5)P(3) gradient substantially exceeds that of the external attractant. An accompanying report presents evidence for a ... More
A hyperspectral fluorescence system for 3D in vivo optical imaging.
AuthorsZavattini G, Vecchi S, Mitchell G, Weisser U, Leahy RM, Pichler BJ, Smith DJ, Cherry SR
JournalPhys Med Biol
PubMed ID16585843
In vivo optical instruments designed for small animal imaging generally measure the integrated light intensity across a broad band of wavelengths, or make measurements at a small number of selected wavelengths, and primarily use any spectral information to characterize and remove autofluorescence. We have developed a flexible hyperspectral imaging instrument ... More
Microtubule-dependent movement of late endocytic vesicles in vitro: requirements for Dynein and Kinesin.
AuthorsBananis E, Nath S, Gordon K, Satir P, Stockert RJ, Murray JW, Wolkoff AW
JournalMol Biol Cell
PubMed ID15181154
Our previous studies demonstrated that fluorescent early endocytic vesicles prepared from rat liver after injection of Texas red asialoorosomucoid contain asialoglycoprotein and its receptor and move and undergo fission along microtubules using kinesin I and KIFC2, with Rab4 regulating KIFC2 activity (J. Cell Sci. 116, 2749, 2003). In the current ... More
Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells.
AuthorsWislet-Gendebien S, Leprince P, Moonen G, Rogister B
JournalJ Cell Sci
PubMed ID12840074
Bone marrow stromal cells can differentiate into many types of mesenchymal cells, i.e. osteocyte, chondrocyte and adipocyte, but can also differentiate into non-mesenchymal cells, i.e. neural cells under appropriate in vivo experimental conditions (Kopen et al., 1999; Brazelton et al., 2000; Mezey et al., 2000). This neural phenotypic plasticity allows ... More
Engineering a disulfide bond to stabilize the calcium-binding loop of activated protein C eliminates its anticoagulant but not its protective signaling properties.
AuthorsBae JS, Yang L, Manithody C, Rezaie AR
JournalJ Biol Chem
PubMed ID17255099
In addition to an anticoagulant activity, activated protein C (APC) also exhibits anti-inflammatory and cytoprotective properties. These properties may contribute to the beneficial effect of APC in treating severe sepsis patients. A higher incidence of bleeding because of its anticoagulant function has been found to be a major drawback of ... More
Auditory Thalamostriatal and Corticostriatal Pathways Convey Complementary Information about Sound Features.
AuthorsPonvert ND, Jaramillo S
JournalJ Neurosci
PubMed ID30459227
'Multiple parallel neural pathways link sound-related signals to behavioral responses. For instance, the striatum, a brain structure involved in action selection and reward-related learning, receives neuronal projections from both the auditory thalamus and auditory cortex. It is not clear whether sound information that reaches the striatum through these two pathways ... More
Rapid Intestinal Uptake and Targeted Delivery to the Liver Endothelium Using Orally Administered Silver Sulfide Quantum Dots.
Authors
JournalACS Nano
PubMed ID31976646
Left Habenula Mediates Light-Preference Behavior in Zebrafish via an Asymmetrical Visual Pathway.
Authors
JournalNeuron
PubMed ID28190643
Nanoscale kinetic segregation of TCR and CD45 in engaged microvilli facilitates early T cell activation.
Authors
JournalNat Commun
PubMed ID29467364
Visualizing mesoderm and neural crest cell dynamics during chick head morphogenesis.
AuthorsMcKinney MC, McLennan R, Giniunaite R, Baker RE, Maini PK, Othmer HG, Kulesa PM
JournalDev Biol
PubMed ID32084354
'Vertebrate head morphogenesis involves carefully-orchestrated tissue growth and cell movements of the mesoderm and neural crest to form the distinct craniofacial pattern. To better understand structural birth defects, it is important that we characterize the dynamics of these processes and learn how they rely on each other. Here we examine ... More
The microprotein Minion controls cell fusion and muscle formation.
Authors
JournalNat Commun
PubMed ID28569745
Single Particle Imaging of Polarized Hepatoma Organoids upon Hepatitis C Virus Infection Reveals an Ordered and Sequential Entry Process.
Authors
JournalCell Host Microbe
PubMed ID29544098
Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair.
Authors
JournalNat Commun
PubMed ID33658506
Identification of the Human Skeletal Stem Cell.
Authors
JournalCell
PubMed ID30241615
In vivo imaging of transplanted hematopoietic stem and progenitor cells in mouse calvarium bone marrow.
Authors
JournalNat Protoc
PubMed ID21212779
Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML.
Authors
JournalCell Stem Cell
PubMed ID29276143
A FoxA2+ long-term stem cell population is necessary for growth plate cartilage regeneration after injury.
Authors
JournalNat Commun
PubMed ID35523895
Tunneling nanotube-mediated intercellular vesicle and protein transfer in the stroma-provided imatinib resistance in chronic myeloid leukemia cells.
AuthorsKolba MD, Dudka W, Zareba-Koziol M, Kominek A, Ronchi P, Turos L, Chroscicki P, Wlodarczyk J, Schwab Y, Klejman A, Cysewski D, Srpan K, Davis DM, Piwocka K
JournalCell Death Dis
PubMed ID31659149
Intercellular communication within the bone marrow niche significantly promotes leukemogenesis and provides protection of leukemic cells from therapy. Secreted factors, intercellular transfer of mitochondria and the receptor-ligand interactions have been shown as mediators of this protection. Here we report that tunneling nanotubes (TNTs)-long, thin membranous structures, which have been identified ... More
Transcriptomic Analysis of Breast Cancer Stem Cells and Development of a pALDH1A1:mNeptune Reporter System for Live Tracking.
AuthorsBidan N, Bailleul-Dubois J, Duval J, Winter M, Denoulet M, Hannebicque K, El-Sayed IY, Ginestier C, Forissier V, Charafe-Jauffret E, Macario M, Matsunaga YT, Meignan S, Anquez F, Julien S, Bonnefond A, Derhourhi M, Le Bourhis X, Lagadec C
JournalProteomics
PubMed ID31430054
Many solid cancers are hierarchically organized with a small number of cancer stem cells (CSCs) able to regrow a tumor, while their progeny lacks this feature. Breast CSC is known to contribute to therapy resistance. The study of those cells is usually based on their cell-surface markers like CD44 ... More
Bone marrow CX3CR1+ mononuclear cells relay a systemic microbiota signal to control hematopoietic progenitors in mice.
AuthorsLee S, Kim H, You G, Kim YM, Lee S, Le VH, Kwon O, Im SH, Kim YM, Kim KS, Sung YC, Kim KH, Surh CD, Park Y, Lee SW
JournalBlood
PubMed ID31387916
The microbiota regulate hematopoiesis in the bone marrow (BM); however, the detailed mechanisms remain largely unknown. In this study, we explored how microbiota-derived molecules (MDMs) were transferred to the BM and sensed by the local immune cells to control hematopoiesis under steady-state conditions. We reveal that MDMs, including bacterial DNA ... More