mTOR-dependent proliferation defect in human ES-derived neural stem cells affected by myotonic dystrophy type 1.
AuthorsDenis JA, Gauthier M, Rachdi L, Aubert S, Giraud-Triboult K, Poydenot P, Benchoua A, Champon B, Maury Y, Baldeschi C, Scharfmann R, Piétu G, Peschanski M, Martinat C,
JournalJ Cell Sci
PubMed ID23444380
'Patients with myotonic dystrophy type 1 exhibit a diversity of symptoms that affect many different organs. Among these are cognitive dysfunctions, the origin of which has remained elusive, partly because of the difficulty in accessing neural cells. Here, we have taken advantage of pluripotent stem cell lines derived from embryos ... More
Glucagon-like peptide 1 stimulates post-translational activation of glucokinase in pancreatic beta cells.
AuthorsDing SY, Nkobena A, Kraft CA, Markwardt ML, Rizzo MA,
JournalJ Biol Chem
PubMed ID21454584
Glucagon-like peptide 1 (GLP-1) potentiates glucose-stimulated insulin secretion from pancreatic ß cells, yet does not directly stimulate secretion. The mechanisms underlying this phenomenon are incompletely understood. Here, we report that GLP-1 augments glucose-dependent rises in NAD(P)H autofluorescence in both ßTC3 insulinoma cells and islets in a manner consistent with post-translational ... More
Nuclear expression of E2F4 induces cell death via multiple pathways in normal human intestinal epithelial crypt cells but not in colon cancer cells.
AuthorsGarneau H, Alvarez L, Paquin MC, Lussier C, Rancourt C, Tremblay E, Beaulieu JF, Rivard N,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID17656449
E2F transcription factors control cell cycle progression. The localization of E2F4 in intestinal epithelial cells is cell cycle dependent, being cytoplasmic in quiescent differentiated cells but nuclear in proliferative cells. However, whether nuclear translocation of E2F4 alone is sufficient to trigger intestinal epithelial cell proliferation remains to be established. Adenoviruses ... More
G protein-coupled receptor-promoted trafficking of Gbeta1gamma2 leads to AKT activation at endosomes via a mechanism mediated by Gbeta1gamma2-Rab11a interaction.
AuthorsGarcía-Regalado A, Guzmán-Hernández ML, Ramírez-Rangel I, Robles-Molina E, Balla T, Vázquez-Prado J, Reyes-Cruz G,
JournalMol Biol Cell
PubMed ID18701709
G-protein coupled receptors activate heterotrimeric G proteins at the plasma membrane in which most of their effectors are intrinsically located or transiently associated as the external signal is being transduced. This paradigm has been extended to the intracellular compartments by studies in yeast showing that trafficking of Galpha activates phosphatidylinositol ... More
Tetramethylpyrazine regulates breast cancer cell viability, migration, invasion and apoptosis by affecting the activity of Akt and caspase-3.
AuthorsShen J, Zeng L, Pan L, Yuan S, Wu M, Kong X
JournalOncol Lett
PubMed ID29541225
Tetramethylpyrazine (TMP), an effective component of the traditional Chinese medicine Chuanxiong Hort, has been proven to exhibit a beneficial effect in a number of types of malignant epithelial cancer. However, the mode of action of TMP on breast cancer cells remains unknown. The aim of the present study was to ... More