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引用和文献 (54)
Invitrogen™
Lipofectin™ 转染试剂
Lipofectin® 转染试剂是内皮细胞转染的首选试剂。Lipofectin® 转染试剂也适用于将 DNA、RNA 和寡核苷酸转染到哺乳动物细胞和将 DNA 转染到植物原生质体中。Lipofectin®了解更多信息
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| 货号 | 数量 |
|---|---|
| 18292037 | 4 mL |
| 18292011 | 1 mL |
货号 18292037
价格（CNY)
26,368.00
Each
数量:
4 mL
价格（CNY)
26,368.00
Each
Lipofectin® 转染试剂是内皮细胞转染的首选试剂。Lipofectin® 转染试剂也适用于将 DNA、RNA 和寡核苷酸转染到哺乳动物细胞和将 DNA 转染到植物原生质体中。Lipofectin® 试剂还显示与 PLUS® 试剂联合可有效用于转染 HeLa 细胞。Lipofectin® 转染试剂是 1:1(w/w) 阳离子脂质 N-[1-(2,3-二油酰氧基)丙基]-n,n,n-三甲基氯化铵 (DOTMA) 和二油酰磷脂酰乙醇胺 (DOPE) 溶于膜过滤水的脂质体制剂。
仅供科研使用。不可用于诊断程序。
规格
适用于（应用）转染
高通量能力不兼容高通量应用（手动）
包括4 瓶（每瓶 1mL）Lipofectin 转染试剂
产品线Lipofectin
产品类型转染试剂
数量4 mL
血清兼容性否
运输条件湿冰
细胞类型已建立的细胞系, 原代细胞, 难转染细胞
产品规格6孔板、12孔板、24孔板、48孔板、96孔板、培养瓶
样品类型质粒 DNA
转染技术脂质转染
Unit SizeEach
内容与储存
每单位包含一个或多个试剂样品瓶 (1 mL)。在 +4°C 下储存。妥善储存时，保证 Lipofectin™ 转染试剂可稳定储存 6 个月。
常见问题解答 (FAQ)
我不慎将我的脂质体试剂留在了室温条件下，还能继续使用吗？
你们是否为内皮细胞的转染操作提供专门试剂？
反向转染与正向转染之间区别是什么？我该如何选择？
转染过程中的培养基可使用抗生素么？
在脂质体转染过程中必须使用无血清的培养基么？
引用和文献 (54)
引用和文献
Abstract
Transfection techniques for producing recombinant baculoviruses.
Journal:Methods Mol Biol
PubMed ID:7620563
A role for calcium in stabilizing transport vesicle coats.
Journal:J Biol Chem
PubMed ID:11435443
'Calcium has been implicated in regulating vesicle fusion reactions, but its potential role in regulating other aspects of protein transport, such as vesicle assembly, is largely unexplored. We find that treating cells with the membrane-permeable calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N'',N''-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), leads to a dramatic redistribution of the vesicle
Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.
Journal:J Biol Chem
PubMed ID:9748285
'p53 is a tumor suppressor protein that controls cell proliferation by regulating the expression of growth control genes. In a previous study, we identified two proteins, 53BP1 and 53BP2, that are able to bind to wild type but not to mutant p53 via the DNA-binding domain of p53. We isolated
Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells.
Journal:J Biol Chem
PubMed ID:8995303
'The inducible cyclooxygenase, COX-2, has been associated with vascular inflammation and cellular proliferation. We have discovered that hypoxia increases expression of the COX-2 gene in human vascular endothelial cells in culture independent of other stimuli. Western analysis of human umbilical vein endothelial cells (HUVEC) revealed a greater than 4-fold induction
Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer.
Journal:Nucleic Acids Res
PubMed ID:1377820
'As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed of a firefly