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引用和文献 (7)
Thermo Scientific™
EMCS（N-ε-马来酰亚胺基己酰-氧琥珀酰亚胺酯）
Thermo Scientific Pierce EMCS 是一种胺对巯基交联剂，含有 NHS 酯和马来酰亚胺反应性基团，位于中等长度间隔臂（9.4 埃）的相对两端了解更多信息
| 货号 | 数量 |
|---|---|
| 22308 | 50 mg |
货号 22308
价格（CNY)
4,497.00
Online Exclusive
Ends: 31-Dec-2025
5,740.00共减 1,243.00 (22%)
Each
数量:
50 mg
价格（CNY)
4,497.00
Online Exclusive
Ends: 31-Dec-2025
5,740.00共减 1,243.00 (22%)
Each
Thermo Scientific Pierce EMCS 是一种胺对巯基交联剂，含有 NHS 酯和马来酰亚胺反应性基团，位于中等长度间隔臂（9.4 埃）的相对两端。
EMCS 的特点：
•反应性基团：NHS 酯和马来酰亚胺
•可与以下基团发生反应：氨基和巯基
•脂肪族间隔系列的 C6 同系物包括 AMAS、BMPS、GMBS 和 EMCS
•NHS 酯末端在 pH 值 7-9 条件下与伯胺偶联，形成稳定酰胺键
•马来酰亚胺在 pH 值 6.5-7.5 条件下与 -SH 基团反应，形成稳定硫醚键
•不可裂解
•不溶于水（首先溶于 DMF 或 DMSO）；与 Sulfo-EMCS 具有可比性
•偶联球体相较于 GMBS 增加
•短脂肪族间隔引发免疫应答的可能性较低
产品参考：
交联剂应用指南 -- 检索本产品的最新参考文献
EMCS 的特点：
•反应性基团：NHS 酯和马来酰亚胺
•可与以下基团发生反应：氨基和巯基
•脂肪族间隔系列的 C6 同系物包括 AMAS、BMPS、GMBS 和 EMCS
•NHS 酯末端在 pH 值 7-9 条件下与伯胺偶联，形成稳定酰胺键
•马来酰亚胺在 pH 值 6.5-7.5 条件下与 -SH 基团反应，形成稳定硫醚键
•不可裂解
•不溶于水（首先溶于 DMF 或 DMSO）；与 Sulfo-EMCS 具有可比性
•偶联球体相较于 GMBS 增加
•短脂肪族间隔引发免疫应答的可能性较低
产品参考：
交联剂应用指南 -- 检索本产品的最新参考文献
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性是
描述EMCS
形式粉末
标记方法化学标记
分子量308.29
聚乙二醇化否
产品线Pierce
数量50 mg
反应一部分马来酰亚胺、NHS 酯
运输条件环境
溶解度DMF、DMSO
间隔臂长9.4 Å
水溶性否
化学反应性胺-巯基
可裂解否
交联剂类型异型双功能团试剂
产品规格标准
产品类型交联剂
间隔子短 (<10 Å)
Unit SizeEach
内容与储存
接收后，请在 4°C 下干燥储存。
常见问题解答 (FAQ)
Can you provide the shelf-life for EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)?
引用和文献 (7)
引用和文献
Abstract
Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12.
Journal:J Biol Chem
PubMed ID:17548345
'Our model of phospholamban (PLB) regulation of the cardiac Ca(2+)-ATPase in sarcoplasmic reticulum (SERCA2a) states that PLB binds to the Ca(2+)-free, E2 conformation of SERCA2a and blocks it from transitioning from E2 to E1, the Ca(2+)-bound state. PLB and Ca(2+) binding to SERCA2a are mutually exclusive, and PLB inhibition of
Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments.
Journal:Clin Chem
PubMed ID:11067810
Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum
Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking.
Journal:J Biol Chem
PubMed ID:22247554
Chemical cross-linking was used to study protein binding interactions between native phospholamban (PLB) and SERCA2a in sarcoplasmic reticulum (SR) vesicles prepared from normal and failed human hearts. Lys(27) of PLB was cross-linked to the Ca(2+) pump at the cytoplasmic extension of M4 (at or near Lys(328)) with the homobifunctional cross-linker,
PNA microarrays for hybridisation of unlabelled DNA samples.
Journal:Nucleic Acids Res
PubMed ID:14500847
Several strategies have been developed for the production of peptide nucleic acid (PNA) microarrays by parallel probe synthesis and selective coupling of full-length molecules. Such microarrays were used for direct detection of the hybridisation of unlabelled DNA by time-of-flight secondary ion mass spectrometry. PNAs were synthesised by an automated process
Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulum.
Journal:J Biol Chem
PubMed ID:15644311
The ability of two loss-of-function mutants, L31A and L31C, of phospholamban (PLB) to bind to and inhibit the Ca(2+) pump of cardiac sarcoplasmic reticulum (SERCA2a) was investigated using a molecular cross-linking approach. Leu(31) of PLB, located at the cytoplasmic membrane boundary, is a critical amino acid shown previously to be