Folding of the striated muscle myosin motor domain.
Authors: Chow Diana; Srikakulam Rajani; Chen Ying; Winkelmann Donald A;
Journal:J Biol Chem
PubMed ID:12110670
'We have investigated the folding of the myosin motor domain using a chimera of an embryonic striated muscle myosin II motor domain fused on its COOH terminus to a thermal stable, fast folding variant of green fluorescent protein (GFP). In in vitro expression assays, the GFP domain of the chimeric ... More
Role of the E1A Rb-binding domain in repression of the NF-kappa B-dependent defense against tumor necrosis factor-alpha.
Authors: Cook James L; Walker Thomas A; Worthen G Scott; Radke Jay R;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12119420
'The adenoviral E1A oncogene sensitizes mammalian cells to tumor necrosis factor-alpha (TNF-alpha), in part by repressing the nuclear factor-kappa B (NF-kappa B)-dependent defense against this cytokine. Other E1A activities involve binding to either p300/cyclic AMP response element-binding protein (CBP) or retinoblastoma (Rb)-family proteins, but the roles of E1A interactions with ... More
Induction of bacterial lipoprotein tolerance is associated with suppression of toll-like receptor 2 expression.
Authors:Wang JH, Doyle M, Manning BJ, Di Wu Q, Blankson S, Redmond HP.
Journal:J Biol Chem
PubMed ID:12133836
'Tolerance to bacterial cell wall components including lipopolysaccharide (LPS) may represent an essential regulatory mechanism during bacterial infection. Two members of the Toll-like receptor (TLR) family, TLR2 and TLR4, recognize the specific pattern of bacterial cell wall components. TLR4 has been found to be responsible for LPS tolerance. However, the ... More
Hepatitis C virus subgenomic replicons in the human embryonic kidney 293 cell line.
Authors:Ali S, Pellerin C, Lamarre D, Kukolj G,
Journal:J Virol
PubMed ID:14671129
'Hepatitis C virus (HCV) infects liver cells and its replication in other cells is incompletely defined. Human hepatoma Huh-7 cells harboring subgenomic HCV replicons were used in somatic cell fusion experiments with human embryonic kidney 293 cells as a means of examining the permissiveness of 293 cells for HCV subgenomic ... More
c-Myc sensitizes cells to tumor necrosis factor-mediated apoptosis by inhibiting nuclear factor kappa B transactivation.
Authors: You Zongbing; Madrid Lee V; Saims Daniel; Sedivy John; Wang Cun-Yu;
Journal:J Biol Chem
PubMed ID:12149248
Nuclear factor kappaB (NF-kappaB) plays a key role in suppression of tumor necrosis factor (TNF)-mediated apoptosis by inducing a variety of anti-apoptotic genes. Expression of c-Myc has been shown to sensitize cells to TNF-mediated apoptosis by inhibiting NF-kappaB activation. However, the precise step in the NF-kappaB signaling pathway and apoptosis ... More