Miser™ 抗体延长液 NC
Thermo Scientific™

Miser™ 抗体延长液 NC

Thermo Scientific Pierce Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much了解更多信息
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货号ValidatedApplication
32110
货号 32110
价格(CNY)
2,507.00
500 mL
请求批量或定制报价
价格(CNY)
2,507.00
500 mL
Thermo Scientific Pierce Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Features of Antibody Extender Solution NC:

Achieve equivalent signal—use at least three times less primary antibody than normal and retain comparable or better signal detectability
Any detection mode or enzyme—the method works with both colorimetric and chemiluminescent detection modes and with horseradish peroxidase (HRP) and alkaline phosphatase (AP) systems
Easy-to-perform, 10-minute protocol—invest a few minutes and save money on your primary antibody; our scientists have reduced their use of expensive primary antibodies by up to 100-fold with this product

By treating nitrocellulose membranes for a few minutes in this reagent immediately after transfer and before probing, you can use much less (more dilute) primary antibody solutions while obtaining the same level of detection. This conserves use of expensive and rare antibodies, enabling more experiments to be performed with the same amount of antibody. The reagent is so effective that our scientists have observed antibody cost reductions of 10-, 25- and even 100-times (depending on the antigen and primary antibody directed against that antigen).
For Research Use Only. Not for use in diagnostic procedures.
规格
适用于(应用)ELISA, Western Blot
形式Liquid
Nitrocellulose Membrane
产品线Miser
产品类型Antibody Extender Solution
Unit Size500 mL
内容与储存
Store at room temperature.

常见问题解答 (FAQ)

我应该使用哪一个:Western Blot信号增强剂还是Miser Antibody Extender Solution NC?

如果你对你的western信号满意,你可以使用Miser Extender Solution NC来减少你需要使用的抗体量,从而降低你的成本。如果你需要增加灵敏度,那么你可以使用Western Blot信号增强剂。

Miser Antibody Extender Solution NC是如何使我能使用更少的抗体的?

膜上的蛋白质更容易被抗体接触,使得使用较少抗体产生信号成为可能。

我能在使用Miser Antibody Extender Solution NC前用丽春红染色吗?

Miser Antibody Extender Solution应在使用丽春红染色后使用。

我能在硝酸纤维素膜和PVDF膜上使用Miser Antibody Extender Solution NC?

Miser Antibody Extender Solution在硝酸纤维素膜上能提供一致的、可重复的结果;在PVDF膜上的结果因膜而异。

在我的实验流程中何时需要使用Miser Antibody Extender Solution NC?

Miser Antibody Extender Solution应在将蛋白质转膜后,封闭前使用。