GlutaMAX™ 补充剂
GlutaMAX™ 补充剂
引用和文献 (4)
Gibco™

GlutaMAX™ 补充剂

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Gibco™ GlutaMAX™ 添加剂是 L-谷氨酰胺的替代品，具有更好的稳定性，可改善细胞健康。GlutaMAX™ 添加剂适合于哺乳动物细胞的贴壁和悬浮培养，而且无需适应。GlutaMAX™ 补充剂以 200 mM了解更多信息
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货号数量
3505007920 x 100 mL
35050061100 mL
货号 35050079
价格（CNY)
14,356.00
Each
添加至购物车
数量:
20 x 100 mL
价格（CNY)
14,356.00
Each
添加至购物车
Gibco™ GlutaMAX™ 添加剂是 L-谷氨酰胺的替代品，具有更好的稳定性，可改善细胞健康。GlutaMAX™ 添加剂适合于哺乳动物细胞的贴壁和悬浮培养，而且无需适应。GlutaMAX™ 补充剂以 200 mM L-丙氨酰-L-谷氨酰胺二肽形式供货，溶剂为 0.85% NaCl。GlutaMAX™ 添加剂也包括在各种培养基配方中。与 L-谷氨酰胺相比，GlutaMAX™ 添加剂：

•可显著减少有毒氨的积累
•改善细胞活力和生长情况
•在很宽的温度范围内保持稳定

可显著减少有毒氨的积累
与 L-谷氨酰胺不同，GlutaMAX™ 添加剂不会自发分解，因此不会形成氨。相反，细胞会根据需要裂解二肽键以释放 L-谷氨酰胺。该系统可以防止废物的积累并在长期培养过程中维持 L-谷氨酰胺的新鲜供应

改善细胞活力和生长情况
氨含量较低的培养物显示出更高的细胞活力。此外，由于 GlutaMAX™ 添加剂具有一致的浓度，因此培养条件更易控制，而使用 L-谷氨酰胺时不可能实现这一点

在很宽的温度范围内保持稳定
GlutaMAX™ 添加剂在室温下稳定并且为即用型。GlutaMAX™ 补充剂可在室温、2 至 8°C 或 -5 至 -30°C 下保留完整功能长达 24 个月，方便储存和处理。GlutaMAX™ 补充剂可在 37°C 下稳定放置一周或更长时间。

cGMP 生产和质量体系
Gibco™ GlutaMAX™ 补充剂在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在FDA登记的医疗器械生产商，且通过ISO 13485标准认证。为确保供应链的稳定，我们同时提供由我们的苏格兰工厂生产的相同 Gibco™ PBS 产品 (35050-087)。该工厂也是在 FDA 注册的医疗器械生产商，且通过 ISO 13485 标准认证。
规格
细胞类型哺乳动物细胞
最大浓度100 X
培养类型哺乳动物细胞培养
适用于（应用）临床研究、转化研究
环保功能绿色可持续包装
生产质量cGMP-compliant under the ISO 13485 standard
渗透压440 - 500 mOsm/kg
产品线Gibco，GlutaMAX
数量20 x 100 mL
有效期24 个月
来源非动物来源
分类无动物来源
形式液体
产品类型GlutaMax
无菌无菌过滤
pH4.7 - 6
Unit SizeEach
内容与储存
储存条件：15°C 至 30°C
可选储存条件：-5°C 至 -20°C 和 2°C 至 8°C
运输条件：室温
有效期：自生产之日起24个月

常见问题解答 (FAQ)

清除，缺少或降解谷氨酰胺或生长因子等促生长的基本成份会对培养物的生长速率造成影响么？

是的。如果您怀疑发生了类似情况，请移去培养基并换用新的培养基。或者，您可以添加含有促生长成份的培养基。也可使用I或II型GlutaMAX取代培养基中的谷氨酰胺，以避免谷氨酰胺被耗竭。

 

配方中含有Gibco GlutaMAX添加剂会改变L-谷氨酰胺的含量么？

在所有以GlutaMAX双肽添加剂作为L-谷氨酰胺替代品的培养基中，谷氨酰胺的浓度都与原始配方中的L-谷氨酰胺浓度处于同等的摩尔水平。

Gibco GlutaMAX与谷氨酰胺添加剂之间的区别是什么？

GlutaMAX-I 添加剂是一款能够改善细胞培养效果的添加剂，是您细胞培养基中L-谷氨酰胺的直接替代品。请点击此处(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html)来阅读更多关于GlutaMAX添加剂性能的详细信息。

Why is GlutaMAX Supplement (Cat. Nos. 35050061, 35050079) recommended over L-glutamine when reconstituting Geltrex Reduced Growth Factor Basement Membrane Matrix?

GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) require supplementation for use?

When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.

Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all GlutaMAX™ 补充剂 FAQs

引用和文献 (4)

引用和文献
Abstract
Directed differentiation of pancreatic δ cells from human pluripotent stem cells.
Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H
Journal:Nature communications
PubMed ID:39068220
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More
Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors.
Authors:Kutner RH, Zhang XY, Reiser J,
Journal:Nat Protoc
PubMed ID:19300443
'Over the past decade, lentiviral vectors have emerged as powerful tools for transgene delivery. The use of lentiviral vectors has become commonplace and applications in the fields of neuroscience, hematology, developmental biology, stem cell biology and transgenesis are rapidly emerging. Also, lentiviral vectors are at present being explored in the ... More
IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Authors:Lu C, Ward PS, Kapoor GS, Rohle D, Turcan S, Abdel-Wahab O, Edwards CR, Khanin R, Figueroa ME, Melnick A, Wellen KE, O'Rourke DM, Berger SL, Chan TA, Levine RL, Mellinghoff IK, Thompson CB,
Journal:Nature
PubMed ID:22343901
Recurrent mutations in isocitrate dehydrogenase 1 (IDH1) and IDH2 have been identified in gliomas, acute myeloid leukaemias (AML) and chondrosarcomas, and share a novel enzymatic property of producing 2-hydroxyglutarate (2HG) from a-ketoglutarate. Here we report that 2HG-producing IDH mutants can prevent the histone demethylation that is required for lineage-specific progenitor ... More
Characterization of pluripotent stem cells.
Authors:Martí M, Mulero L, Pardo C, Morera C, Carrió M, Laricchia-Robbio L, Esteban CR, Izpisua Belmonte JC,
Journal:Nat Protoc
PubMed ID:23306458
Characterization of pluripotent stem cells is required for the registration of stem cell lines and allows for an impartial and objective comparison of the results obtained when generating multiple lines. It is therefore crucial to establish specific, fast and reliable protocols to detect the hallmarks of pluripotency. Such protocols should ... More