Custom TaqMan™ 基因表达Assay，FAM

Yes, you can order it as a custom assay. Since the assay is discontinued, it can not be ordered on-line. You will have to call or email the order to genomics@appliedbiosystems.com, and include the assay ID. The assay will be synthesized once the order is received. It will be designed and priced as a Custom TaqMan Gene Expression Assay so you will need to choose the scale.

No, the assay is not detecting different genes. Genes often have multiple valid accession numbers, even for the exact same sequence. Even though there are different ID numbers listed, they should all represent the same gene.

The label on the TaqMan Gene Expression Assays lists the expiration date, which under proper storage conditions is 5 yrs. The assay itself is guaranteed for 1 yr upon shipmentt.

We often design multiple assays for the same target. The difference between the assay IDs is most often that the assay is designed across a different exon-exon boundary of a particular transcript sequence. You can confirm the exon-exon boundary by clicking on the individual Assay ID and then look at the Interrogated Sequence Table.

Another difference between assays for the same gene is that they detect different mRNA species or splice variants for the same gene. Ensure that the assay you select will detect your transcripts of interest.

No, the Custom TaqMan Gene Expression Assays are only synthesized with FAM dye as the reporter dye at the 5' end and MGB-NFQ as the quencher at the 3' end. Dye selection is only possible with general custom oligo synthesis.

In order to take advantage of our superior in-house assay design algorithm, we recommend that you order a small scale of the standard Custom TaqMan Gene Expression Assay with the FAM label first and validate the assay in a single-plex reaction. You can then submit the sequences of the primers and probes for custom oligo synthesis where you will have the flexibility of choosing your reporter dye.